Publication: PI(3,5)P<sub>2</sub> asymmetry during mitosis is essential for asymmetric vacuolar inheritance
dc.contributor.department | Department of Molecular Biology and Genetics | |
dc.contributor.department | Graduate School of Sciences and Engineering | |
dc.contributor.kuauthor | Çaydaşı, Ayşe Koca | |
dc.contributor.kuauthor | Dilege, Cansu | |
dc.contributor.kuauthor | Koyuncu, Mukadder | |
dc.contributor.schoolcollegeinstitute | College of Sciences | |
dc.contributor.schoolcollegeinstitute | GRADUATE SCHOOL OF SCIENCES AND ENGINEERING | |
dc.date.accessioned | 2025-03-06T20:58:59Z | |
dc.date.issued | 2024 | |
dc.description.abstract | Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is a low-abundance signaling lipid that plays crucial roles in various cellular processes, including endolysosomal system structure/function, stress response, and cell cycle regulation. PI(3,5)P2 synthesis increases in response to environmental stimuli, yet its behavior in cycling cells under basal conditions remains elusive. Here, we analyzed spatiotemporal changes in PI(3,5)P2 levels during the cell cycle of S. cerevisiae. We found that PI(3,5)P2 accumulates on the vacuole in the daughter cell while it disappears from the vacuole in the mother cell during mitosis. Concomitant with the changes in PI(3,5)P2 distribution, the daughter vacuole became more acidic, whereas the acidity of the mother vacuole decreased during mitosis. Our data further showed that both PI(3,5)P2 and the PI(3,5)P2 effector protein Atg18 are determinants of vacuolar-pH asymmetry and acidity. Our work, thus, identifies PI(3,5)P2 as a key factor for the establishment of vacuolar-pH asymmetry, providing insights into how the mother cell ages while the daughter cell is rejuvenated. | |
dc.description.indexedby | WOS | |
dc.description.indexedby | PubMed | |
dc.description.publisherscope | International | |
dc.description.sponsoredbyTubitakEu | EU - TÜBİTAK | |
dc.description.sponsorship | We would like to thank Gislene Pereira (University of Heidelberg, Heidelberg, Germany), Elmar Schiebel (University of Heidelberg, Heidelberg, Germany), Andreas Mayer (University of Lausanne, Lausanne, Switzerland), Keisuke Obara (Nagoya University, Nagoya, Japan), Yoshinori Ohsumi (Tokyo Institute of Technology, Tokyo, Japan), Daniel Gottschling (Calico Life Sciences, San Francisco, CA, USA), and Voytek Okreglak (Altos Labs, Redwood City, CA, USA) for sharing yeast strains and plasmids. We are grateful to Jason King (University of Sheffield, Sheffield, UK) for sharing the sequence of the SnxA reporter prior to its release. This study was supported by European Molecular Biology Organization installation Grant No. 3918, and Turkiye Bilimsel ve TeknolojikAras,t & imath;rma Kurumu (TUBITAK) Grant No. 219Z100. M. Huda and M. Koyuncu were funded by TUBITAK Grant No. 122Z887, 219Z100, and 118Z979. | |
dc.identifier.doi | 10.1083/jcb.202406170 | |
dc.identifier.eissn | 1540-8140 | |
dc.identifier.grantno | European Molecular Biology Organization installation [3918];Turkiye Bilimsel ve TeknolojikAras,timath;rma Kurumu (TUBITAK) [219Z100];TUBITAK [219Z100, 122Z887, 118Z979] | |
dc.identifier.issn | 0021-9525 | |
dc.identifier.issue | 1 | |
dc.identifier.quartile | Q1 | |
dc.identifier.uri | https://doi.org/10.1083/jcb.202406170 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14288/27601 | |
dc.identifier.volume | 224 | |
dc.identifier.wos | 1350271500001 | |
dc.keywords | Endolysosomal system | |
dc.keywords | Cell cycle regulation | |
dc.keywords | S. cerevisiae | |
dc.keywords | Mitosis | |
dc.language.iso | eng | |
dc.publisher | Rockefeller University Press | |
dc.relation.ispartof | Journal of Cell Biology | |
dc.subject | Cell biology | |
dc.title | PI(3,5)P<sub>2</sub> asymmetry during mitosis is essential for asymmetric vacuolar inheritance | |
dc.type | Journal Article | |
dspace.entity.type | Publication | |
local.contributor.kuauthor | Dilege, Cansu | |
local.contributor.kuauthor | Çaydaşı, Ayşe Koca | |
local.contributor.kuauthor | Koyuncu, Mukadder | |
local.contributor.kuauthor | Huda,Mariam | |
local.publication.orgunit1 | GRADUATE SCHOOL OF SCIENCES AND ENGINEERING | |
local.publication.orgunit1 | College of Sciences | |
local.publication.orgunit2 | Department of Molecular Biology and Genetics | |
local.publication.orgunit2 | Graduate School of Sciences and Engineering | |
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