Publication:
PI(3,5)P<sub>2</sub> asymmetry during mitosis is essential for asymmetric vacuolar inheritance

dc.contributor.departmentDepartment of Molecular Biology and Genetics
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.kuauthorÇaydaşı, Ayşe Koca
dc.contributor.kuauthorDilege, Cansu
dc.contributor.kuauthorKoyuncu, Mukadder
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.date.accessioned2025-03-06T20:58:59Z
dc.date.issued2024
dc.description.abstractPhosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is a low-abundance signaling lipid that plays crucial roles in various cellular processes, including endolysosomal system structure/function, stress response, and cell cycle regulation. PI(3,5)P2 synthesis increases in response to environmental stimuli, yet its behavior in cycling cells under basal conditions remains elusive. Here, we analyzed spatiotemporal changes in PI(3,5)P2 levels during the cell cycle of S. cerevisiae. We found that PI(3,5)P2 accumulates on the vacuole in the daughter cell while it disappears from the vacuole in the mother cell during mitosis. Concomitant with the changes in PI(3,5)P2 distribution, the daughter vacuole became more acidic, whereas the acidity of the mother vacuole decreased during mitosis. Our data further showed that both PI(3,5)P2 and the PI(3,5)P2 effector protein Atg18 are determinants of vacuolar-pH asymmetry and acidity. Our work, thus, identifies PI(3,5)P2 as a key factor for the establishment of vacuolar-pH asymmetry, providing insights into how the mother cell ages while the daughter cell is rejuvenated.
dc.description.indexedbyWOS
dc.description.indexedbyPubMed
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuEU - TÜBİTAK
dc.description.sponsorshipWe would like to thank Gislene Pereira (University of Heidelberg, Heidelberg, Germany), Elmar Schiebel (University of Heidelberg, Heidelberg, Germany), Andreas Mayer (University of Lausanne, Lausanne, Switzerland), Keisuke Obara (Nagoya University, Nagoya, Japan), Yoshinori Ohsumi (Tokyo Institute of Technology, Tokyo, Japan), Daniel Gottschling (Calico Life Sciences, San Francisco, CA, USA), and Voytek Okreglak (Altos Labs, Redwood City, CA, USA) for sharing yeast strains and plasmids. We are grateful to Jason King (University of Sheffield, Sheffield, UK) for sharing the sequence of the SnxA reporter prior to its release. This study was supported by European Molecular Biology Organization installation Grant No. 3918, and Turkiye Bilimsel ve TeknolojikAras,t & imath;rma Kurumu (TUBITAK) Grant No. 219Z100. M. Huda and M. Koyuncu were funded by TUBITAK Grant No. 122Z887, 219Z100, and 118Z979.
dc.identifier.doi10.1083/jcb.202406170
dc.identifier.eissn1540-8140
dc.identifier.grantnoEuropean Molecular Biology Organization installation [3918];Turkiye Bilimsel ve TeknolojikAras,timath;rma Kurumu (TUBITAK) [219Z100];TUBITAK [219Z100, 122Z887, 118Z979]
dc.identifier.issn0021-9525
dc.identifier.issue1
dc.identifier.quartileQ1
dc.identifier.urihttps://doi.org/10.1083/jcb.202406170
dc.identifier.urihttps://hdl.handle.net/20.500.14288/27601
dc.identifier.volume224
dc.identifier.wos1350271500001
dc.keywordsEndolysosomal system
dc.keywordsCell cycle regulation
dc.keywordsS. cerevisiae
dc.keywordsMitosis
dc.language.isoeng
dc.publisherRockefeller University Press
dc.relation.ispartofJournal of Cell Biology
dc.subjectCell biology
dc.titlePI(3,5)P<sub>2</sub> asymmetry during mitosis is essential for asymmetric vacuolar inheritance
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorDilege, Cansu
local.contributor.kuauthorÇaydaşı, Ayşe Koca
local.contributor.kuauthorKoyuncu, Mukadder
local.contributor.kuauthorHuda,Mariam
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1College of Sciences
local.publication.orgunit2Department of Molecular Biology and Genetics
local.publication.orgunit2Graduate School of Sciences and Engineering
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