Publication: Resensitization to colistin results in rapid and stable recovery of adherence, serum resistance and ompW in Acinetobacter baumannii
dc.contributor.coauthor | Menekşe, Şirin | |
dc.contributor.department | Department of Industrial Engineering | |
dc.contributor.department | Graduate School of Health Sciences | |
dc.contributor.department | KUH (Koç University Hospital) | |
dc.contributor.department | KUIS AI (Koç University & İş Bank Artificial Intelligence Center) | |
dc.contributor.department | School of Medicine | |
dc.contributor.kuauthor | Boral, Jale | |
dc.contributor.kuauthor | Can, Füsun | |
dc.contributor.kuauthor | Gönen, Mehmet | |
dc.contributor.kuauthor | Keske, Şiran | |
dc.contributor.kuauthor | Özcan, Gülin | |
dc.contributor.kuauthor | Vatansever, Cansel | |
dc.contributor.schoolcollegeinstitute | College of Engineering | |
dc.contributor.schoolcollegeinstitute | GRADUATE SCHOOL OF HEALTH SCIENCES | |
dc.contributor.schoolcollegeinstitute | KUH (KOÇ UNIVERSITY HOSPITAL) | |
dc.contributor.schoolcollegeinstitute | Research Center | |
dc.contributor.schoolcollegeinstitute | SCHOOL OF MEDICINE | |
dc.date.accessioned | 2024-12-29T09:39:29Z | |
dc.date.issued | 2024 | |
dc.description.abstract | Background Colistin resistance in Acinetobacter baumannii is an emerging problem that limits antimicrobial therapy options. Materials & methods We isolated two pairs of colistin susceptible and colistin-resistant A. baumannii (K1007/ K1006 and K408/K409) from two patients diagnosed with carbapenem-resistant A. baumannii infection. Colistin susceptible isolates were exposed to in vitro colistin induction for 50 generations. The selected cell populations were subjected to DNA and RNA sequencing and phenotypic assays. Results In the in vitro induction assay, K408 gained colistin resistance on the corresponding day of clinical resistance (K408-G25) and got resensitized to colistin in the consecutive generation (K408-G26). A significant upregulation of ompW, ata, adeFGH genes on K408-G25 was followed by a downregulation upon resensitization to colistin (G26). Despite the upregulation of the ompW gene in transcriptomic analysis, the ompW protein disappeared on K408-G25 and recovered in the resensitized generation (G26). In parallel, disrupted cell membrane integrity recovered in K408-G26. In the K408-G25, downregulation of pbpG and upregulation of pbp1a/pbp3 genes decreased serum-resistance which was reversed in the resensitized generation (G26). The K1007 did not gain colistin resistance amongst 50-generations, however, the generation corresponding to clinical resistance day (K1007-G9) had a similar trend with K408-G25. The clinical colistin-resistant K409 and K1006 had SNPs on pmrA and pmrB genes. Conclusion In this study, we observed that A. baumannii regulates adhesion, efflux pumps and serum-resistance associated genes as an early response to colistin stress. Besides, the ompW protein disappears in the cell membrane of colistin resistant cells which recovers after resensitization to colistin. The lack of ompW protein in colistin-resistant cells should be taken into consideration for escape mutants in development of antivirulence vaccination or treatment options. © 2024 Public Library of Science. All rights reserved. | |
dc.description.indexedby | WOS | |
dc.description.indexedby | Scopus | |
dc.description.indexedby | PubMed | |
dc.description.issue | 8 | |
dc.description.openaccess | Gold Open Access | |
dc.description.publisherscope | International | |
dc.description.sponsoredbyTubitakEu | TÜBİTAK | |
dc.description.sponsorship | This study was funded by the Scientific and Technological Research Council of Turkey (TUBITAK) under the project number of 119S446 to the corresponding author Fusun Can. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | |
dc.description.volume | 19 | |
dc.identifier.doi | 10.1371/journal.pone.0309307 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.quartile | Q1 | |
dc.identifier.scopus | 2-s2.0-85202672388 | |
dc.identifier.uri | https://doi.org/10.1371/journal.pone.0309307 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14288/23003 | |
dc.identifier.wos | 1305462200031 | |
dc.keywords | Acinetobacter baumannii | |
dc.keywords | Acinetobacter infections | |
dc.keywords | Anti-bacterial agents | |
dc.keywords | Bacterial adhesion | |
dc.keywords | Bacterial outer membrane Proteins | |
dc.keywords | carbapenems | |
dc.keywords | Colistin | |
dc.keywords | Drug resistance, Bacterial | |
dc.keywords | Gene expression Regulation, Bacterial | |
dc.keywords | Humans | |
dc.keywords | Microbial sensitivity tests | |
dc.language.iso | eng | |
dc.publisher | Public Library of Science | |
dc.relation.grantno | 119S446 | |
dc.relation.ispartof | Public Library of Science | |
dc.subject | Resistance and antimicrobials | |
dc.title | Resensitization to colistin results in rapid and stable recovery of adherence, serum resistance and ompW in Acinetobacter baumannii | |
dc.type | Journal Article | |
dspace.entity.type | Publication | |
local.contributor.kuauthor | Boral, Jale | |
local.contributor.kuauthor | Vatansever, Cansel | |
local.contributor.kuauthor | Özcan, Gülin | |
local.contributor.kuauthor | Keske, Şiran | |
local.contributor.kuauthor | Gönen, Mehmet | |
local.contributor.kuauthor | Can, Füsun | |
local.publication.orgunit1 | College of Engineering | |
local.publication.orgunit1 | GRADUATE SCHOOL OF HEALTH SCIENCES | |
local.publication.orgunit1 | SCHOOL OF MEDICINE | |
local.publication.orgunit1 | Research Center | |
local.publication.orgunit1 | KUH (KOÇ UNIVERSITY HOSPITAL) | |
local.publication.orgunit2 | Department of Industrial Engineering | |
local.publication.orgunit2 | KUIS AI (Koç University & İş Bank Artificial Intelligence Center) | |
local.publication.orgunit2 | KUH (Koç University Hospital) | |
local.publication.orgunit2 | School of Medicine | |
local.publication.orgunit2 | Graduate School of Health Sciences | |
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