Generation and hematopoietic differentiation of mesenchymal stromal/stem cell-derived induced pluripotent stem cell lines for disease modeling of hematopoietic and immunological diseases

Abstract

Here, we describe a protocol for reprogramming of bone marrow–derived multipotent mesenchymal stromal/stem cells to obtain induced pluripotent stem cells from patients with primary immune deficiencies using lentiviral vectors, followed by hematopoietic differentiation of the MSC-derived iPSCs. This protocol is particularly helpful in cases where it is difficult to obtain sufficient numbers of hematopoietic cells for research and can be applied to model any hematological/immunological disease. © 2021, Springer Science+Business Media, LLC.