Publication: Generation and hematopoietic differentiation of mesenchymal stromal/stem cell-derived induced pluripotent stem cell lines for disease modeling of hematopoietic and immunological diseases
| dc.contributor.coauthor | Aerts-Kaya, Fatima | |
| dc.contributor.kuauthor | Esken, Gülen Güney | |
| dc.date.accessioned | 2024-11-09T22:48:43Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Here, we describe a protocol for reprogramming of bone marrow–derived multipotent mesenchymal stromal/stem cells to obtain induced pluripotent stem cells from patients with primary immune deficiencies using lentiviral vectors, followed by hematopoietic differentiation of the MSC-derived iPSCs. This protocol is particularly helpful in cases where it is difficult to obtain sufficient numbers of hematopoietic cells for research and can be applied to model any hematological/immunological disease. © 2021, Springer Science+Business Media, LLC. | |
| dc.description.indexedby | Scopus | |
| dc.description.indexedby | PubMed | |
| dc.description.openaccess | YES | |
| dc.description.publisherscope | International | |
| dc.description.sponsoredbyTubitakEu | N/A | |
| dc.description.volume | 2549 | |
| dc.identifier.doi | 10.1007/7651_2021_452 | |
| dc.identifier.issn | 1064-3745 | |
| dc.identifier.scopus | 2-s2.0-85133525952 | |
| dc.identifier.uri | https://doi.org/10.1007/7651_2021_452 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14288/6373 | |
| dc.keywords | Differentiation | |
| dc.keywords | Hematopoiesis | |
| dc.keywords | Hematopoietic stem cells | |
| dc.keywords | Induced pluripotent stem cells | |
| dc.keywords | Mesenchymal stem cells antibody | |
| dc.keywords | Buffer | |
| dc.keywords | Growth factor | |
| dc.keywords | Cell differentiation | |
| dc.keywords | Coculture | |
| dc.keywords | Controlled study | |
| dc.keywords | Flow cytometry | |
| dc.keywords | Fluorescence activated cell sorting | |
| dc.keywords | Freezing | |
| dc.keywords | Hematologic disease | |
| dc.keywords | Hematopoiesis | |
| dc.keywords | Human | |
| dc.keywords | Human cell | |
| dc.keywords | Human cell culture | |
| dc.keywords | Immune deficiency | |
| dc.keywords | Immunofluorescence assay | |
| dc.keywords | Immunophenotyping | |
| dc.keywords | Induced pluripotent stem cell | |
| dc.keywords | Mesenchymal stroma cell | |
| dc.keywords | Nuclear reprogramming | |
| dc.keywords | OP9 cell line | |
| dc.keywords | Stem cell | |
| dc.keywords | Stem cell culture | |
| dc.keywords | Thawing | |
| dc.keywords | Cell differentiation | |
| dc.keywords | Hematopoietic stem cell | |
| dc.keywords | Immunopathology | |
| dc.keywords | Mesenchymal stem cell | |
| dc.keywords | metabolism | |
| dc.keywords | Humans | |
| dc.keywords | Immune system diseases | |
| dc.keywords | Induced pluripotent stem cells | |
| dc.keywords | Mesenchymal stem cells | |
| dc.language.iso | eng | |
| dc.publisher | Humana Press Inc. | |
| dc.relation.ispartof | Methods in Molecular Biology | |
| dc.subject | Induced pluripotent stem cells | |
| dc.subject | Nuclear reprogramming | |
| dc.subject | Cell differentiation | |
| dc.title | Generation and hematopoietic differentiation of mesenchymal stromal/stem cell-derived induced pluripotent stem cell lines for disease modeling of hematopoietic and immunological diseases | |
| dc.type | Book Chapter | |
| dspace.entity.type | Publication | |
| local.contributor.kuauthor | Esken, Gülen Güney | |
| person.familyName | Esken | |
| person.givenName | Gülen Güney |