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Generation and hematopoietic differentiation of mesenchymal stromal/stem cell-derived induced pluripotent stem cell lines for disease modeling of hematopoietic and immunological diseases

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dc.contributor.coauthorAerts-Kaya, Fatima
dc.contributor.departmentN/A
dc.contributor.kuauthorEsken, Gülen Güney
dc.contributor.kuprofileOther
dc.contributor.schoolcollegeinstituteN/A
dc.contributor.yokidN/A
dc.date.accessioned2024-11-09T22:48:43Z
dc.date.issued2022
dc.description.abstractHere, we describe a protocol for reprogramming of bone marrow–derived multipotent mesenchymal stromal/stem cells to obtain induced pluripotent stem cells from patients with primary immune deficiencies using lentiviral vectors, followed by hematopoietic differentiation of the MSC-derived iPSCs. This protocol is particularly helpful in cases where it is difficult to obtain sufficient numbers of hematopoietic cells for research and can be applied to model any hematological/immunological disease. © 2021, Springer Science+Business Media, LLC.
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.volume2549
dc.identifier.doi10.1007/7651_2021_452
dc.identifier.issn1064-3745
dc.identifier.linkhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85133525952&doi=10.1007%2f7651_2021_452&partnerID=40&md5=195135b56eac77ced7a5087b905c97e5
dc.identifier.scopus2-s2.0-85133525952
dc.identifier.urihttp://dx.doi.org/10.1007/7651_2021_452
dc.identifier.urihttps://hdl.handle.net/20.500.14288/6373
dc.keywordsDifferentiation
dc.keywordsHematopoiesis
dc.keywordsHematopoietic stem cells
dc.keywordsInduced pluripotent stem cells
dc.keywordsMesenchymal stem cells antibody
dc.keywordsBuffer
dc.keywordsGrowth factor
dc.keywordsCell differentiation
dc.keywordsCoculture
dc.keywordsControlled study
dc.keywordsFlow cytometry
dc.keywordsFluorescence activated cell sorting
dc.keywordsFreezing
dc.keywordsHematologic disease
dc.keywordsHematopoiesis
dc.keywordsHuman
dc.keywordsHuman cell
dc.keywordsHuman cell culture
dc.keywordsImmune deficiency
dc.keywordsImmunofluorescence assay
dc.keywordsImmunophenotyping
dc.keywordsInduced pluripotent stem cell
dc.keywordsMesenchymal stroma cell
dc.keywordsNuclear reprogramming
dc.keywordsOP9 cell line
dc.keywordsStem cell
dc.keywordsStem cell culture
dc.keywordsThawing
dc.keywordsCell differentiation
dc.keywordsHematopoietic stem cell
dc.keywordsImmunopathology
dc.keywordsMesenchymal stem cell
dc.keywordsmetabolism
dc.keywordsHumans
dc.keywordsImmune system diseases
dc.keywordsInduced pluripotent stem cells
dc.keywordsMesenchymal stem cells
dc.languageEnglish
dc.publisherHumana Press Inc.
dc.sourceMethods in Molecular Biology
dc.subjectInduced pluripotent stem cells
dc.subjectNuclear reprogramming
dc.subjectCell differentiation
dc.titleGeneration and hematopoietic differentiation of mesenchymal stromal/stem cell-derived induced pluripotent stem cell lines for disease modeling of hematopoietic and immunological diseases
dc.typeBook Chapter
dspace.entity.typePublication
local.contributor.authorid0000-0002-3158-1587
local.contributor.kuauthorEsken, Gülen Güney

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