Publication: Constrained thermal denaturation of DNA under fixed linking number
Program
KU-Authors
KU Authors
Co-Authors
Bar, Amir
Mukamel, David
Publication Date
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Type
Embargo Status
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Volume Title
Alternative Title
Abstract
A DNA molecule with freely fluctuating ends undergoes a sharp thermal denaturation transition upon heating. However, in circular DNA chains and some experimental setups that manipulate single DNA molecules, the total number of turns (linking number) is constant at all times. The consequences of this additional topological invariant on the melting behaviour are nontrivial. Below, we investigate the melting characteristics of a homogeneous DNA where the linking number along the melting curve is preserved by supercoil formation in duplex portions. We obtain the mass fraction and the number of loops and supercoils below and above the melting temperature. We also argue that a macroscopic loop appears at T (c) and calculate its size as a function of temperature.
Source
Publisher
De Gruyter
Subject
Physics
Citation
Has Part
Source
Central European Journal of Physics
Book Series Title
Edition
DOI
10.2478/s11534-012-0070-7