Publication:
Effect of interferon alfa-2a treatment on adaptive and innate immune systems in patients with Behçet disease uveitis

dc.contributor.coauthorOray, Merih
dc.contributor.coauthorKırımlı, Günay Uludağ
dc.contributor.coauthorGül, Ahmet
dc.contributor.coauthorTuğal-Tutkun, İlknur
dc.contributor.departmentKUH (Koç University Hospital)
dc.contributor.departmentSchool of Medicine
dc.contributor.departmentGraduate School of Health Sciences
dc.contributor.kuauthorAlbayrak, Özgür
dc.contributor.kuauthorCan, Füsun
dc.contributor.kuauthorÖnal, Sumru
dc.contributor.kuprofileFaculty Member
dc.contributor.kuprofileOther
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF HEALTH SCIENCES
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.contributor.schoolcollegeinstituteKUH (KOÇ UNIVERSITY HOSPITAL)
dc.contributor.yokidN/A
dc.contributor.yokid103165
dc.contributor.yokid52359
dc.date.accessioned2024-11-09T12:13:55Z
dc.date.issued2019
dc.description.abstractPurpose: to investigate the effect of interferon alfa-2a on T regulatory (Treg) cells, T helper 17 (Th17) cells, and expression of Toll-like receptors (TLRs) in Behçet disease (BD) patients with uveitis. Methods: twenty-seven patients who received interferon alfa-2a for active BD uveitis despite conventional immunomodulatory therapies and healthy controls were enrolled. Peripheral blood Treg and Th17 cell frequencies were determined by flow cytometry as gated cells for CD3 + CD4 + Foxp3 + and CD3 + CD4 + IL17A + , respectively. Th17 RAR-related orphan receptor (ROR)γt mRNA expression was verified by real-time PCR (RT-PCR). Treg and Th17 cell cytokines were detected by ELISA in the supernatant of short-term cell cultures. RT-PCR was used to assess expression of TLR-2, TLR-3, TLR-4, TLR-8, and TLR-9 using cDNA prepared from CD4 + T cells and monocytes. Results: Treg and Th17 cell frequencies and Th17 RORγt expression were significantly elevated, and IL-10 concentration in Treg cell supernatants was significantly lower in BD patients than in controls. Th17 IL-17, IL-6, IL-21, IL-22, IL-23, IFN-γ, and TNF-α concentrations were significantly higher and all TLR expressions were significantly elevated in patients. Interferon alfa-2a led to a significant reversal in Treg and Th17 cell frequencies, Th17 RORγt expression, Treg and Th17 cell cytokine production, and TLR expression by CD4 + T cells and monocytes. Conclusions: despite a relative increase in Treg cells, impaired IL-10 production suggests that Treg dysfunction may play a role in induction of BD uveitis. Favorable effects of interferon alfa-2a may be associated with recovery of Treg cell function, suppression of Th17 cells, and reduced expression of TLRs on CD4 + T cells and monocytes.
dc.description.fulltextYES
dc.description.indexedbyWoS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue1
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuTÜBİTAK
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TÜBİTAK)
dc.description.versionPublisher version
dc.description.volume60
dc.formatpdf
dc.identifier.doi10.1167/iovs.18-25548
dc.identifier.embargoNO
dc.identifier.filenameinventorynoIR01613
dc.identifier.issn0146-0404
dc.identifier.linkhttps://doi.org/10.1167/iovs.18-25548
dc.identifier.quartileQ1
dc.identifier.scopus2-s2.0-85059492722
dc.identifier.urihttps://hdl.handle.net/20.500.14288/1262
dc.identifier.wos454713900006
dc.keywordsAdaptive
dc.keywordsBehçet disease
dc.keywordsInnate
dc.keywordsInterferons
dc.keywordsUveitis
dc.languageEnglish
dc.publisherAssociation for Research in Vision and Ophthalmology (ARVO)
dc.relation.grantno115S541
dc.relation.urihttp://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/8240
dc.sourceInvestigative Ophthalmology and Visual Science
dc.subjectMedicine
dc.subjectOphthalmology
dc.titleEffect of interferon alfa-2a treatment on adaptive and innate immune systems in patients with Behçet disease uveitis
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.authoridN/A
local.contributor.authorid0000-0001-9387-2526
local.contributor.authorid0000-0002-4036-922X
local.contributor.kuauthorAlbayrak, Özgür
local.contributor.kuauthorCan, Füsun
local.contributor.kuauthorÖnal, Sumru
local.publication.orgunit1GRADUATE SCHOOL OF HEALTH SCIENCES
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1KUH (KOÇ UNIVERSITY HOSPITAL)
local.publication.orgunit2KUH (Koç University Hospital)
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Health Sciences
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