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Real-time visualization of cholesterol trafficking in human granulosa cells using confocal live cell microscopy as a tool to study the novel role of autophagy in sex steroid synthesis

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dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.departmentAnimal Laboratory
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorEsmaeilian, Yashar
dc.contributor.kuauthorÖktem, Özgür
dc.contributor.schoolcollegeinstituteLaboratory
dc.contributor.schoolcollegeinstituteResearch Center
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-12-29T09:38:55Z
dc.date.issued2024
dc.description.abstractAutophagy is an evolutionarily conserved process that aims to maintain the energy homeostasis of the cell by recycling long-lived proteins and organelles. We have very recently demonstrated that lipophagy, a special form of autophagy, mediates the association of the lipid droplets (LDs) with lysosomes to deliver the lipid cargo within the LDs to lysosomes for degradation in order to release free cholesterol required for steroid synthesis in human ovary and testis. In this chapter, we describe live cell confocal microscopy technique that allows us to monitor real-time cholesterol trafficking and the association of cholesterol-laden LDs with lysosome (lipophagy) in human granulosa cells.
dc.description.indexedbyPubMed
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.identifier.doi10.1007/7651_2024_521
dc.identifier.eissn1940-6029
dc.identifier.issn1064-3745
dc.identifier.quartileN/A
dc.identifier.urihttps://doi.org/10.1007/7651_2024_521
dc.identifier.urihttps://hdl.handle.net/20.500.14288/22853
dc.keywordsAutophagy
dc.keywordsCholesterol
dc.keywordsConfocal microscopy
dc.keywordsImageJ
dc.keywordsLipid droplet
dc.keywordsLive cell imaging
dc.keywordsLuteinized granulosa cell
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofMethods Mol Biol
dc.subjectMedicine
dc.titleReal-time visualization of cholesterol trafficking in human granulosa cells using confocal live cell microscopy as a tool to study the novel role of autophagy in sex steroid synthesis
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorEsmaeilian, Yashar
local.contributor.kuauthorÖktem, Özgür
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1Research Center
local.publication.orgunit1Laboratory
local.publication.orgunit2KUTTAM (Koç University Research Center for Translational Medicine)
local.publication.orgunit2Animal Laboratory
local.publication.orgunit2School of Medicine
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