Centriolar satellites are dynamic membrane-less organelles that assemble via a hierarchical pathway

Abstract

Centriolar satellites (CS) are ubiquitous, membrane-less organelles recognized for their dynamic organelle crosstalk, plasticity, diverse functions and links to developmental and neuronal diseases. Despite their significance, the mechanisms underlying CS assembly and homeostasis are unknown. Here, we developed in vitro and cellular CS biogenesis assays to spatiotemporally quantify the homeostatic properties of CS granules during assembly and maintenance. These assays revealed that CS assemble via a hierarchical pathway initiated by PCM1 scaffold formation followed by sequential recruitment of CS proteins. We discovered that PCM1 inherently forms granules through multimerization and phase separation, processes regulated by cytoskeleton and ciliopathy proteins. Additionally, PCM1 scaffold and clients are organized into subdomains within CS granules with distinct composition and dynamics. Selectively disrupting CS granule properties impaired the ciliary signaling functions of CS. Collectively, our results provide comprehensive insight into CS biogenesis and establish a conceptual framework and new tools to investigate context-dependent CS functions and their deregulation in disease. The mechanisms identified for CS may also explain the specificity and plasticity of other membrane-less organelles.