Evaluation of white matter integrity by using diffusion tensor imaging in spontaneous intracranial hypotension

Abstract

Objective: The aim is to investigate whether there is a microstructural change in the white matter pathways in patients with spontaneous intracranial hypotension (SIH). Additionally, the relationship between conventional magnetic resonance imaging (MRI) findings and diffusion tensor imaging (DTI) parameters is determined. Methods: Thirty patients diagnosed with SIH and 31 control patients (between January 2019 and February 2024) were included in the case-control study. MRI findings constituting the Bern score were evaluated in contrast-enhanced brain MRI. Ten different white matter pathways were evaluated with the following DTI parameters: fractional anisotropy (FA), apparent diffusion coefficient (ADC), axial diffusion (AD), and radial diffusion (RD). We also examined the association between MRI findings and DTI parameters. Results: In the cingulum, a decreased FA value (Z = -3.18, p = 0.002) was observed in patients with SIH, indicating disruption of white matter integrity and weakened synaptic transmission in this region. In the corona radiata, significant reductions were observed in both FA (Z = -5.67, p < 0.001) and AD (Z = -3.59, p < 0.001), alongside an increase in RD (Z = -4.99, p < 0.001). The concomitant increase in RD further supports the idea of potential damage to the myelin sheath, which, together with other findings, may reflect disruptions in white matter architecture that underlie the cognitive and motor deficits frequently seen in patients with SIH. In the splenium of the corpus callosum, a significant reduction in AD (Z = -3.00, p = 0.007) was also noted. In the anterior limb of the internal capsule (ALIC), both FA (Z = -4.57, p < 0.001) and AD (Z = -2.73, p = 0.012) values decreased, while RD increased (Z = -2.93, p = 0.007). These alterations suggest the involvement of motor and sensory pathways. In the posterior limb of the internal capsule (PLIC), significant reductions were found in both FA (Z = -5.59, p < 0.001) and AD (Z = -3.82, p < 0.001), along with an increase in RD (Z = -5.49, p < 0.001). In the corticospinal tract, patients with SIH exhibited a decrease in FA (Z = -4.15, p < 0.001), an increase in ADC (Z = -2.87, p = 0.008), and an increase in RD (Z = -3.73, p < 0.001). These findings represent significant microstructural changes reflecting a reduction in the integrity of motor pathways in white matter. In the middle cerebellar peduncle (MCP), patients with SIH showed increased ADC (Z = -4.78, p < 0.001) and RD (Z = -3.23, p = 0.002). Similarly, in optic radiation, there was decreased FA (Z = -4.55, p < 0.001) and AD (Z = -2.59, p = 0.018), with increased RD (Z = -2.95, p = 0.007). In the correlation analysis, positive correlations were found between suprasellar distance and AD values in both the ALIC (r(s) = 0.451, p = 0.012) and MCP (r(s) = 0.416, p = 0.022). These findings suggest that structural changes and anatomical shifts, especially in the suprasellar region, may be associated with microstructural changes in the adjacent white matter pathways. Conclusion: We revealed microstructural changes in white matter in SIH. The alterations in DTI parameters may suggest that the white matter microarchitecture is changed as a result of mechanical compression of the axons in the white matter pathways due to brain prolapse and venous congestion.