Publication: The mammalian target of rapamycin protein expression in human granulosa cell tumors
dc.contributor.coauthor | Güralp, Onur | |
dc.contributor.coauthor | Bese, Tugan | |
dc.contributor.coauthor | Demikıran, Fuat | |
dc.contributor.coauthor | İnce, Ümit | |
dc.contributor.coauthor | Malik, Eduard | |
dc.contributor.coauthor | Arvas, Macit | |
dc.contributor.department | School of Medicine | |
dc.contributor.department | Graduate School of Health Sciences | |
dc.contributor.kuauthor | Bildik, Gamze | |
dc.contributor.kuauthor | Öktem, Özgür | |
dc.contributor.schoolcollegeinstitute | GRADUATE SCHOOL OF HEALTH SCIENCES | |
dc.contributor.schoolcollegeinstitute | SCHOOL OF MEDICINE | |
dc.date.accessioned | 2024-11-09T11:59:22Z | |
dc.date.issued | 2019 | |
dc.description.abstract | Objective: to investigate the role of mammalian target of rapamycin (mTOR) in human granulosa cell ovarian tumors and the therapeutic effect of rapamycin in COV434 mitotic granulosa cell lines. Material and Methods: a retrospective evaluation of the medical records and pathologic sections of patients with granulosa cell ovarian carcinoma was performed. mTOR and p-mTOR expression was immunohistochemically investigated. A COV434 cell culture were treated with 0.5, 1, 2, and 5 mu M rapamycin. Real-time growth curve analysis via xCELLigence system and apoptotic cell analysis via YO-PROT-1 Iodide were performed to assess the therapeutic effect of rapamycin on cancer cells. Results: a total of twenty patients were evaluated. mTOR staining was detected in 18 (90%) patients. Mild, moderate, intense, and very intense staining was observed in three (15%), eight (40%), six (30%), and one (5%) sample, respectively. The mean mTOR staining ratio was 59 +/- 41%. P-mTOR staining was observed in two ( 10%) patients. One (5%) patient had 5% staining, and one (5%) patient had 100% staining for p-mTOR. Both of the latter patients had very intense staining. Rapamycin caused a dose-dependent growth arrest and induced apoptosis in COV434 mitotic granulosa cells. The real-time growth curves of the cells treated with these drugs were distinguished by a marked reduced slope after exposure for several hours, indicating a rapid onset of apoptosis. Live/dead cell analysis with YO-PRO-1 staining showed that rapamycin induced apoptosis in 24% of the cells when used at 1 mu M concentration, whereas the rate increased to 61% and 72% when the cells were treated with 2 mu M and 5 mu M rapamycin, respectively. Conclusion: mTOR expression is observed in various degrees in 90%, and p-mTOR expression is observed in only 10% of patients with granulosa cell ovarian carcinoma. Rapamycin caused a dose-dependent growth arrest and apoptosis in COV434 mitotic granulosa cells. | |
dc.description.fulltext | YES | |
dc.description.indexedby | WOS | |
dc.description.indexedby | Scopus | |
dc.description.indexedby | PubMed | |
dc.description.issue | 4 | |
dc.description.openaccess | YES | |
dc.description.publisherscope | National | |
dc.description.sponsoredbyTubitakEu | N/A | |
dc.description.sponsorship | İstanbul University, Scientific Research Fund | |
dc.description.version | Publisher version | |
dc.description.volume | 20 | |
dc.identifier.doi | 10.4274/jtgga.galenos.2018.2018.0140 | |
dc.identifier.eissn | 1309-0380 | |
dc.identifier.embargo | NO | |
dc.identifier.filenameinventoryno | IR02026 | |
dc.identifier.issn | 1309-0399 | |
dc.identifier.quartile | N/A | |
dc.identifier.scopus | 2-s2.0-85075866439 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14288/921 | |
dc.identifier.wos | 499667400008 | |
dc.keywords | Granulosa cell ovarian tumor | |
dc.keywords | mTOR | |
dc.keywords | Rapamycin | |
dc.keywords | Ovarian cancer | |
dc.language.iso | eng | |
dc.publisher | Galenos Yayınevi | |
dc.relation.grantno | 17384 | |
dc.relation.ispartof | Journal of the Turkish- German Gynecology Association | |
dc.relation.uri | http://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/8677 | |
dc.subject | Medicine | |
dc.subject | Obstetrics and gynecology | |
dc.title | The mammalian target of rapamycin protein expression in human granulosa cell tumors | |
dc.type | Journal Article | |
dspace.entity.type | Publication | |
local.contributor.kuauthor | Öktem, Özgür | |
local.contributor.kuauthor | Bildik, Gamze | |
local.publication.orgunit1 | SCHOOL OF MEDICINE | |
local.publication.orgunit1 | GRADUATE SCHOOL OF HEALTH SCIENCES | |
local.publication.orgunit2 | School of Medicine | |
local.publication.orgunit2 | Graduate School of Health Sciences | |
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relation.isOrgUnitOfPublication | 2f870f28-12c9-4b28-9465-b91a69c1d48c | |
relation.isOrgUnitOfPublication.latestForDiscovery | d02929e1-2a70-44f0-ae17-7819f587bedd | |
relation.isParentOrgUnitOfPublication | 4c75e0a5-ca7f-4443-bd78-1b473d4f6743 | |
relation.isParentOrgUnitOfPublication | 17f2dc8e-6e54-4fa8-b5e0-d6415123a93e | |
relation.isParentOrgUnitOfPublication.latestForDiscovery | 4c75e0a5-ca7f-4443-bd78-1b473d4f6743 |
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