Publication:
The mammalian target of rapamycin protein expression in human granulosa cell tumors

dc.contributor.coauthorGüralp, Onur
dc.contributor.coauthorBese, Tugan
dc.contributor.coauthorDemikıran, Fuat
dc.contributor.coauthorİnce, Ümit
dc.contributor.coauthorMalik, Eduard
dc.contributor.coauthorArvas, Macit
dc.contributor.departmentSchool of Medicine
dc.contributor.departmentGraduate School of Health Sciences
dc.contributor.kuauthorBildik, Gamze
dc.contributor.kuauthorÖktem, Özgür
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF HEALTH SCIENCES
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T11:59:22Z
dc.date.issued2019
dc.description.abstractObjective: to investigate the role of mammalian target of rapamycin (mTOR) in human granulosa cell ovarian tumors and the therapeutic effect of rapamycin in COV434 mitotic granulosa cell lines. Material and Methods: a retrospective evaluation of the medical records and pathologic sections of patients with granulosa cell ovarian carcinoma was performed. mTOR and p-mTOR expression was immunohistochemically investigated. A COV434 cell culture were treated with 0.5, 1, 2, and 5 mu M rapamycin. Real-time growth curve analysis via xCELLigence system and apoptotic cell analysis via YO-PROT-1 Iodide were performed to assess the therapeutic effect of rapamycin on cancer cells. Results: a total of twenty patients were evaluated. mTOR staining was detected in 18 (90%) patients. Mild, moderate, intense, and very intense staining was observed in three (15%), eight (40%), six (30%), and one (5%) sample, respectively. The mean mTOR staining ratio was 59 +/- 41%. P-mTOR staining was observed in two ( 10%) patients. One (5%) patient had 5% staining, and one (5%) patient had 100% staining for p-mTOR. Both of the latter patients had very intense staining. Rapamycin caused a dose-dependent growth arrest and induced apoptosis in COV434 mitotic granulosa cells. The real-time growth curves of the cells treated with these drugs were distinguished by a marked reduced slope after exposure for several hours, indicating a rapid onset of apoptosis. Live/dead cell analysis with YO-PRO-1 staining showed that rapamycin induced apoptosis in 24% of the cells when used at 1 mu M concentration, whereas the rate increased to 61% and 72% when the cells were treated with 2 mu M and 5 mu M rapamycin, respectively. Conclusion: mTOR expression is observed in various degrees in 90%, and p-mTOR expression is observed in only 10% of patients with granulosa cell ovarian carcinoma. Rapamycin caused a dose-dependent growth arrest and apoptosis in COV434 mitotic granulosa cells.
dc.description.fulltextYES
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue4
dc.description.openaccessYES
dc.description.publisherscopeNational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipİstanbul University, Scientific Research Fund
dc.description.versionPublisher version
dc.description.volume20
dc.identifier.doi10.4274/jtgga.galenos.2018.2018.0140
dc.identifier.eissn1309-0380
dc.identifier.embargoNO
dc.identifier.filenameinventorynoIR02026
dc.identifier.issn1309-0399
dc.identifier.quartileN/A
dc.identifier.scopus2-s2.0-85075866439
dc.identifier.urihttps://hdl.handle.net/20.500.14288/921
dc.identifier.wos499667400008
dc.keywordsGranulosa cell ovarian tumor
dc.keywordsmTOR
dc.keywordsRapamycin
dc.keywordsOvarian cancer
dc.language.isoeng
dc.publisherGalenos Yayınevi
dc.relation.grantno17384
dc.relation.ispartofJournal of the Turkish- German Gynecology Association
dc.relation.urihttp://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/8677
dc.subjectMedicine
dc.subjectObstetrics and gynecology
dc.titleThe mammalian target of rapamycin protein expression in human granulosa cell tumors
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorÖktem, Özgür
local.contributor.kuauthorBildik, Gamze
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1GRADUATE SCHOOL OF HEALTH SCIENCES
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Health Sciences
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