Publication:
Roles of developmentally regulated KIF2A alternative isoforms in cortical neuron migration and differentiation

dc.contributor.departmentDepartment of Molecular Biology and Genetics
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.kuauthorAkarlar, Büşra
dc.contributor.kuauthorAkkaya, Cansu
dc.contributor.kuauthorAtak, Dila
dc.contributor.kuauthorBayam, Efil
dc.contributor.kuauthorDunn, Gülayşe İnce
dc.contributor.kuauthorGüner, Gökhan
dc.contributor.kuauthorKamacıoğlu, Altuğ
dc.contributor.kuauthorTaşkın, Ali Cihan
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteResearch Center
dc.date.accessioned2024-11-09T22:53:32Z
dc.date.issued2021
dc.description.abstractKIF2A is a kinesin motor protein with essential roles in neural progenitor division and axonal pruning during brain development. However, how different KIF2A alternative isoforms function during development of the cerebral cortex is not known. Here, we focus on three Kif2a isoforms expressed in the developing cortex. We show that Kif2a is essential for dendritic arborization in mice and that the functions of all three isoforms are sufficient for this process. Interestingly, only two of the isoforms can sustain radial migration of cortical neurons; a third isoform, lacking a key N-terminal region, is ineffective. By proximity-based interactome mapping for individual isoforms, we identify previously known KIF2A interactors, proteins localized to the mitotic spindle poles and, unexpectedly, also translation factors, ribonucleoproteins and proteins that are targeted to organelles, prominently to the mitochondria. In addition, we show that a KIF2A mutation, which causes brain malformations in humans, has extensive changes to its proximity-based interactome, with depletion of mitochondrial proteins identified in the wild-type KIF2A interactome. Our data raises new insights about the importance of alternative splice variants during brain development.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue4
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipEuropean Commission Seventh Framework Programme
dc.description.sponsorshipInternational Reintegration
dc.description.sponsorshipScientific and Technical Research Council of Turkey (TÜBİTAK)
dc.description.sponsorshipBIDEB 2211/E Scholarship Program
dc.description.volume148
dc.identifier.doi10.1242/dev.192674
dc.identifier.eissn1477-9129
dc.identifier.issn0950-1991
dc.identifier.quartileQ1
dc.identifier.scopus2-s2.0-85102222604
dc.identifier.urihttps://doi.org/10.1242/dev.192674
dc.identifier.urihttps://hdl.handle.net/20.500.14288/7208
dc.identifier.wos627114300007
dc.keywordsAlternative splicing
dc.keywordsKIF2A
dc.keywordsCortical development
dc.keywordsProximity-interactome mapping
dc.language.isoeng
dc.publisherThe Company of Biologists
dc.relation.grantnoPIRG07-GA-2010-268433
dc.relation.grantno115Z707
dc.relation.ispartofDevelopment
dc.subjectDevelopmental biology
dc.titleRoles of developmentally regulated KIF2A alternative isoforms in cortical neuron migration and differentiation
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorAkkaya, Cansu
local.contributor.kuauthorAtak, Dila
local.contributor.kuauthorKamacıoğlu, Altuğ
local.contributor.kuauthorAkarlar, Büşra
local.contributor.kuauthorGüner, Gökhan
local.contributor.kuauthorBayam, Efil
local.contributor.kuauthorTaşkın, Ali Cihan
local.contributor.kuauthorÖzlü, Nurhan
local.contributor.kuauthorDunn, Gülayşe İnce
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1College of Sciences
local.publication.orgunit1Research Center
local.publication.orgunit2Department of Molecular Biology and Genetics
local.publication.orgunit2KUTTAM (Koç University Research Center for Translational Medicine)
local.publication.orgunit2Graduate School of Sciences and Engineering
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