Publication:
Directing chemiluminescent dioxetanes to mitochondria: a cationic luminophore enables in vitro and in vivo detection of cancer cells upon enzymatic activation

dc.contributor.departmentDepartment of Chemistry
dc.contributor.departmentGraduate School of Health Sciences
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorAcari, Alperen
dc.contributor.kuauthorAlmammadov, Toghrul
dc.contributor.kuauthorÇetin, Sultan
dc.contributor.kuauthorCingöz, Ahmet
dc.contributor.kuauthorDeğirmenci, Nareg Pınarbaşı
dc.contributor.kuauthorDırak, Musa
dc.contributor.kuauthorGündüz, Hande
dc.contributor.kuauthorKılıç, Eda
dc.contributor.kuauthorKölemen, Safacan
dc.contributor.kuauthorÖnder, Tuğba Bağcı
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF HEALTH SCIENCES
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteResearch Center
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T23:59:18Z
dc.date.issued2023
dc.description.abstractA mitochondrion targeted and leucine aminopeptidase (LAP) activatable 1,2-dioxatane based chemiluminescent probe (MCL) for detection of LAP activity in living cancer cells and tumor bearing mice was reported. MCL displayed a selective and sensitive turn-on response in aqueous solutions upon reacting with the LAP enzyme. In cell culture studies, a selective luminescence intensity increase was observed in cancer cell lines, suggesting that MCL can differentiate between cancer and normal cells and allows detection of varying endogenous LAP concentrations. Using fluorescence imaging with a commercial Mitotracker dye, MCL was also shown to localize mitochondria in cancer cell lines. Furthermore, MCL was used to image tumors in mice models. MCL marks not only the first ever example of a mitochondria targeted chemiluminescent probe, but also the first ever example of an organelle targeted 1,2-dioxetane derivative. © 2023 Elsevier B.V.
dc.description.indexedbyScopus
dc.description.indexedbyWOS
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.volume383
dc.identifier.doi10.1016/j.snb.2023.133574
dc.identifier.issn0925-4005
dc.identifier.quartileQ1
dc.identifier.scopus2-s2.0-85150924040
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85150924040&doi=10.1016%2fj.snb.2023.133574&partnerID=40&md5=5805a53711c903b1832c76ec45791971
dc.identifier.wos978190500001
dc.keywordsChemiluminescence
dc.keywordsDioxetanes
dc.keywordsLeucine aminopeptidase
dc.keywordsMitochondria
dc.keywordsTumor imaging
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofSensors and Actuators B: Chemical
dc.subjectChemistry
dc.subjectAnalytical
dc.subjectElectrochemistry
dc.subjectInstruments
dc.subjectInstrumentation
dc.titleDirecting chemiluminescent dioxetanes to mitochondria: a cationic luminophore enables in vitro and in vivo detection of cancer cells upon enzymatic activation
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorGündüz, Hande
local.contributor.kuauthorAcari, Alperen
local.contributor.kuauthorÇetin, Sultan
local.contributor.kuauthorAlmammadov, Toghrul
local.contributor.kuauthorDeğirmenci, Nareg Pınarbaşı
local.contributor.kuauthorDırak, Musa
local.contributor.kuauthorCingöz, Ahmet
local.contributor.kuauthorKılıç, Eda
local.contributor.kuauthorÖnder, Tuğba Bağcı
local.contributor.kuauthorKölemen, Safacan
local.publication.orgunit1College of Sciences
local.publication.orgunit1GRADUATE SCHOOL OF HEALTH SCIENCES
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1Research Center
local.publication.orgunit2Department of Chemistry
local.publication.orgunit2KUTTAM (Koç University Research Center for Translational Medicine)
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Health Sciences
local.publication.orgunit2Graduate School of Sciences and Engineering
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