The effect of silibinin on liver healing following blunt trauma in rats silibininin künt karaciğer travmalı sıçan modelinde karaciğer iyileşmesi üzerindeki etkisi

Abstract

ROUND: This study aimed to evaluate the effects of silibinin, a compound with demonstrated therapeutic effects in various liver diseases, on liver healing in a rat model of blunt liver trauma. METHODS: Twenty-four Wistar albino rats weighing 250-300 g were randomly assigned to three equal groups. Group I served as the control group (CG) and received standard nutrition without trauma induction. Under general anesthesia, rats in Groups II and III underwent a blunt trauma procedure applied to the right upper abdominal quadrant. Group II, designated as the trauma group (TG), received standard nutrition only. Group III, designated as the trauma-silibinin group (T-SG), received silibinin at a dose of 100 mg/kg/day via gastric gavage for five days in addition to standard nutrition. Following the five-day observation period, blood samples were collected from inferior vena cava. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured in the collected blood specimens. Histopathological examination of liver tissue was performed to assess the degree of inflammation. RESULTS: Mean AST and ALT levels differed significantly among the groups (p<0.001). Although no statistically significant difference was observed in inflammation scores between the TG and T-SG groups (p=0.115), inflammation scores tended to be higher in the TG, with a moderate effect size (r=0.41). Furthermore, the risk of a high injury score was 11.7 times greater in the TG than in the T-SG. CONCLUSION: To our knowledge, this is the priority study to evaluate the effects of silibinin in a rat model of blunt liver trauma. Silibinin may represent a promising therapeutic agent for reducing inflammation following liver trauma and promoting liver healing. AMA&#xc7;: Bu ara&#x15f;t&#x131;rma, &#xe7;e&#x15f;itli hastal&#x131;klarda karaci&#x11f;er iyile&#x15f;mesini destekleyen bir madde olan silibininin, k&#xfc;nt karaci&#x11f;er travmas&#x131; modeli kullan&#x131;larak karaci&#x11f;er iyile&#x15f;me s&#xfc;reci &#xfc;zerindeki etkilerini de&#x11f;erlendirmeye odaklanm&#x131;&#x15f;t&#x131;r. GERE&#xc7; VE Y&#xd6;NTEM: Ortalama a&#x11f;&#x131;rl&#x131;klar&#x131; 250-300 g aras&#x131;nda de&#x11f;i&#x15f;en yirmi d&#xf6;rt Wistar Albino s&#x131;&#xe7;an, rastgele ve e&#x15f;it olarak &#xfc;&#xe7; gruba ayr&#x131;ld&#x131;: Grup I, II ve III. Grup I, kontrol grubu (CG), travmaya maruz b&#x131;rak&#x131;lmad&#x131; ve standart beslenme uyguland&#x131;. Genel anestezi alt&#x131;nda, Grup II ve III&#x2019;teki s&#x131;&#xe7;anlara sa&#x11f; &#xfc;st abdominal kadrana k&#xfc;nt travma uyguland&#x131;. Grup II&#x2019;ye, travma grubu (TG), standart beslenme verildi. Grup III, travma-silibinin grubu (T-SG), be&#x15f; g&#xfc;n boyunca g&#xfc;nde 100 mg/kg silibinin mide sondas&#x131; (gavaj) yoluyla ve standart beslenme ile birlikte ald&#x131;. Be&#x15f; g&#xfc;nl&#xfc;k g&#xf6;zlem s&#xfc;resinin ard&#x131;ndan, kan &#xf6;rnekleri vena kava inferior &#xfc;zerinden al&#x131;nd&#x131;. Al&#x131;nan kan &#xf6;rneklerinde serum alanin aminotransferaz (ALT) ve aspartat aminotransferaz (AST) d&#xfc;zeyleri analiz edildi. Karaci&#x11f;er dokusundaki inflamasyon derecesini de&#x11f;erlendirmek amac&#x131;yla histopatolojik inceleme ger&#xe7;ekle&#x15f;tirildi. BULGULAR: Gruplar aras&#x131;ndaki ortalama AST ve ALT de&#x11f;erlerinin kar&#x15f;&#x131;la&#x15f;t&#x131;r&#x131;lmas&#x131;nda istatistiksel olarak anlaml&#x131; bir fark saptand&#x131; (p<0.001). Travmatik gruplar (TG ve T-SG) kar&#x15f;&#x131;la&#x15f;t&#x131;r&#x131;ld&#x131;&#x11f;&#x131;nda inflamasyon skorlar&#x131; a&#xe7;&#x131;s&#x131;ndan istatistiksel olarak anlaml&#x131; bir farkl&#x131;l&#x131;k g&#xf6;zlenmedi (p=0.115). Ancak, travma grubunda daha y&#xfc;ksek skor e&#x11f;ilimi saptand&#x131; ve bu orta d&#xfc;zeyde bir etki b&#xfc;y&#xfc;kl&#xfc;&#x11f;&#xfc;ne sahipti (r=0.41). Ayr&#x131;ca, y&#xfc;ksek hasar skoru g&#xf6;r&#xfc;lme riski, T-SG grubuna k&#x131;yasla travma grubunda 11.7 kat daha fazlayd&#x131;. SONU&#xc7;: Bildi&#x11f;imiz kadar&#x131;yla bu &#xe7;al&#x131;&#x15f;ma, silibininin k&#xfc;nt karaci&#x11f;er travmal&#x131; s&#x131;&#xe7;an modelindeki etkilerini de&#x11f;erlendiren &#xf6;ncelikli bir ara&#x15f;t&#x131;rmad&#x131;r. Silibininin, karaci&#x11f;er travmas&#x131;nda inflamasyonu azaltma ve iyile&#x15f;meyi destekleme a&#xe7;&#x131;s&#x131;ndan potansiyel olarak faydal&#x131; bir ajan olabilece&#x11f;i d&#xfc;&#x15f;&#xfc;n&#xfc;lmektedir.