Publication: Tuning the color palette of fluorescent copper sensors through systematic heteroatom substitution at rhodol cores
dc.contributor.coauthor | Jia, Shang | |
dc.contributor.coauthor | Ramos-Torres, Karla M. | |
dc.contributor.coauthor | Ackerman, Cheri M. | |
dc.contributor.coauthor | Chang, Christopher J. | |
dc.contributor.department | Department of Chemistry | |
dc.contributor.kuauthor | Kölemen, Safacan | |
dc.contributor.schoolcollegeinstitute | College of Sciences | |
dc.date.accessioned | 2024-11-09T22:50:42Z | |
dc.date.issued | 2018 | |
dc.description.abstract | Copper is an essential nutrient for sustaining life, and emerging data have expanded the roles of this metal in biology from its canonical functions as a static enzyme cofactor to dynamic functions as a transition metal signal. At the same time, loosely bound, labile copper pools can trigger oxidative stress and damaging events that are detrimental if misregulated. The signal/stress dichotomy of copper motivates the development of new chemical tools to study its spatial and temporal distributions in native biological contexts such as living cells. Here, we report a family of fluorescent copper sensors built upon carbon-, silicon-, and phosphorus-substituted rhodol dyes that enable systematic tuning of excitation/emission colors from orange to near-infrared. These probes can detect changes in labile copper levels in living cells upon copper supplementation and/or depletion. We demonstrate the ability of the carbon-rhodol based congener, Copper Carbo Fluor 1 (CCF1), to identify elevations in labile copper pools in the Atp7a(-/- )fibroblast cell model of the genetic copper disorder Menkes disease. Moreover, we showcase the utility of the red-emitting phosphorus-rhodol based dye Copper Phosphorus Fluor 1 (CPF1) in dual-color, dual-analyte imaging experiments with the green-emitting calcium indicator Calcium Green-1 to enable simultaneous detection of fluctuations in copper and calcium pools in living cells. The results provide a starting point for advancing tools to study the contributions of copper to health and disease and for exploiting the rapidly growing palette of heteroatom-substituted xanthene dyes to rationally tune the optical properties of fluorescent indicators for other biologically important analytes. | |
dc.description.indexedby | WOS | |
dc.description.indexedby | Scopus | |
dc.description.indexedby | PubMed | |
dc.description.issue | 7 | |
dc.description.openaccess | YES | |
dc.description.publisherscope | International | |
dc.description.sponsoredbyTubitakEu | N/A | |
dc.description.sponsorship | National Institutes of Health [GM79465] | |
dc.description.sponsorship | Chemical Biology Training Grant from the National Institutes of Health [T32 GM066698] | |
dc.description.sponsorship | TUBITAK | |
dc.description.sponsorship | Hertz Foundation We thank the National Institutes of Health (GM79465) for supporting this work. C.J.C. is an Investigator with the Howard Hughes Medical Institute. K.M.R.-T. and C.M.A. were partially supported by a Chemical Biology Training Grant from the National Institutes of Health (T32 GM066698). S.K. thanks TUBITAKfor postdoctoral support. C.M.A. was supported by a Hertz Foundation Graduate Fellowship. We thank M. J. Petris (University of Missouri) for providing the MEF Atp7a knockout and wild-type cells. | |
dc.description.volume | 13 | |
dc.identifier.doi | 10.1021/acschembio.7b00748 | |
dc.identifier.eissn | 1554-8937 | |
dc.identifier.issn | 1554-8929 | |
dc.identifier.quartile | Q2 | |
dc.identifier.scopus | 2-s2.0-85046805698 | |
dc.identifier.uri | https://doi.org/10.1021/acschembio.7b00748 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14288/6719 | |
dc.identifier.wos | 439761900015 | |
dc.language.iso | eng | |
dc.publisher | American Chemical Society (ACS) | |
dc.relation.ispartof | Acs Chemical Biology | |
dc.subject | Biochemistry | |
dc.subject | Molecular Biology | |
dc.title | Tuning the color palette of fluorescent copper sensors through systematic heteroatom substitution at rhodol cores | |
dc.type | Journal Article | |
dspace.entity.type | Publication | |
local.contributor.kuauthor | Kölemen, Safacan | |
local.publication.orgunit1 | College of Sciences | |
local.publication.orgunit2 | Department of Chemistry | |
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relation.isOrgUnitOfPublication.latestForDiscovery | 035d8150-86c9-4107-af16-a6f0a4d538eb | |
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