Publication:
Tuning the color palette of fluorescent copper sensors through systematic heteroatom substitution at rhodol cores

dc.contributor.coauthorJia, Shang
dc.contributor.coauthorRamos-Torres, Karla M.
dc.contributor.coauthorAckerman, Cheri M.
dc.contributor.coauthorChang, Christopher J.
dc.contributor.departmentDepartment of Chemistry
dc.contributor.kuauthorKölemen, Safacan
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.date.accessioned2024-11-09T22:50:42Z
dc.date.issued2018
dc.description.abstractCopper is an essential nutrient for sustaining life, and emerging data have expanded the roles of this metal in biology from its canonical functions as a static enzyme cofactor to dynamic functions as a transition metal signal. At the same time, loosely bound, labile copper pools can trigger oxidative stress and damaging events that are detrimental if misregulated. The signal/stress dichotomy of copper motivates the development of new chemical tools to study its spatial and temporal distributions in native biological contexts such as living cells. Here, we report a family of fluorescent copper sensors built upon carbon-, silicon-, and phosphorus-substituted rhodol dyes that enable systematic tuning of excitation/emission colors from orange to near-infrared. These probes can detect changes in labile copper levels in living cells upon copper supplementation and/or depletion. We demonstrate the ability of the carbon-rhodol based congener, Copper Carbo Fluor 1 (CCF1), to identify elevations in labile copper pools in the Atp7a(-/- )fibroblast cell model of the genetic copper disorder Menkes disease. Moreover, we showcase the utility of the red-emitting phosphorus-rhodol based dye Copper Phosphorus Fluor 1 (CPF1) in dual-color, dual-analyte imaging experiments with the green-emitting calcium indicator Calcium Green-1 to enable simultaneous detection of fluctuations in copper and calcium pools in living cells. The results provide a starting point for advancing tools to study the contributions of copper to health and disease and for exploiting the rapidly growing palette of heteroatom-substituted xanthene dyes to rationally tune the optical properties of fluorescent indicators for other biologically important analytes.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue7
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipNational Institutes of Health [GM79465]
dc.description.sponsorshipChemical Biology Training Grant from the National Institutes of Health [T32 GM066698]
dc.description.sponsorshipTUBITAK
dc.description.sponsorshipHertz Foundation We thank the National Institutes of Health (GM79465) for supporting this work. C.J.C. is an Investigator with the Howard Hughes Medical Institute. K.M.R.-T. and C.M.A. were partially supported by a Chemical Biology Training Grant from the National Institutes of Health (T32 GM066698). S.K. thanks TUBITAKfor postdoctoral support. C.M.A. was supported by a Hertz Foundation Graduate Fellowship. We thank M. J. Petris (University of Missouri) for providing the MEF Atp7a knockout and wild-type cells.
dc.description.volume13
dc.identifier.doi10.1021/acschembio.7b00748
dc.identifier.eissn1554-8937
dc.identifier.issn1554-8929
dc.identifier.quartileQ2
dc.identifier.scopus2-s2.0-85046805698
dc.identifier.urihttps://doi.org/10.1021/acschembio.7b00748
dc.identifier.urihttps://hdl.handle.net/20.500.14288/6719
dc.identifier.wos439761900015
dc.language.isoeng
dc.publisherAmerican Chemical Society (ACS)
dc.relation.ispartofAcs Chemical Biology
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.titleTuning the color palette of fluorescent copper sensors through systematic heteroatom substitution at rhodol cores
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorKölemen, Safacan
local.publication.orgunit1College of Sciences
local.publication.orgunit2Department of Chemistry
relation.isOrgUnitOfPublication035d8150-86c9-4107-af16-a6f0a4d538eb
relation.isOrgUnitOfPublication.latestForDiscovery035d8150-86c9-4107-af16-a6f0a4d538eb
relation.isParentOrgUnitOfPublicationaf0395b0-7219-4165-a909-7016fa30932d
relation.isParentOrgUnitOfPublication.latestForDiscoveryaf0395b0-7219-4165-a909-7016fa30932d

Files