Publication: Remotely guided immunobots engaged in anti-tumorigenic phenotypes for targeted cancer immunotherapy
dc.contributor.coauthor | Doğan, Nihal Olcay | |
dc.contributor.coauthor | Ceylan, Hakan | |
dc.contributor.coauthor | Suadiye, Eylül | |
dc.contributor.coauthor | Sheehan, Devin | |
dc.contributor.coauthor | Aydın, Aslı | |
dc.contributor.coauthor | Yaşa, İmmihan Ceren | |
dc.contributor.coauthor | Wild, Anna-Maria | |
dc.contributor.coauthor | Richter, Gunther | |
dc.contributor.department | Department of Mechanical Engineering | |
dc.contributor.department | School of Medicine | |
dc.contributor.kuauthor | Sitti, Metin | |
dc.contributor.schoolcollegeinstitute | College of Engineering | |
dc.contributor.schoolcollegeinstitute | SCHOOL OF MEDICINE | |
dc.date.accessioned | 2024-11-09T12:43:06Z | |
dc.date.issued | 2022 | |
dc.description.abstract | Building medical microrobots from the body's own cells may circumvent the biocompatibility concern and hence presents more potential in clinical applications to improve the possibility of escaping from the host defense mechanism. More importantly, live cells can enable therapeutically relevant functions with significantly higher efficiency than synthetic systems. Here, live immune cell-derived microrobots from macrophages, i.e., immunobots, which can be remotely steered with externally applied magnetic fields and directed toward anti-tumorigenic (M1) phenotypes, are presented. Macrophages engulf the engineered magnetic decoy bacteria, composed of 0.5 mu m diameter silica Janus particles with one side coated with anisotropic FePt magnetic nanofilm and the other side coated with bacterial lipopolysaccharide (LPS). This study demonstrates the torque-based surface rolling locomotion of the immunobots along assigned trajectories inside blood plasma, over a layer of endothelial cells, and under physiologically relevant flow rates. The immunobots secrete signature M1 cytokines, IL-12 p40, TNF-alpha, and IL-6, and M1 cell markers, CD80 and iNOS, via toll-like receptor 4 (TLR4)-mediated stimulation with bacterial LPS. The immunobots exhibit anticancer activity against urinary bladder cancer cells. This study further demonstrates such immunobots from freshly isolated primary bone marrow-derived macrophages since patient-derivable macrophages may have a strong clinical potential for future cell therapies in cancer. | |
dc.description.fulltext | YES | |
dc.description.indexedby | WOS | |
dc.description.indexedby | Scopus | |
dc.description.indexedby | PubMed | |
dc.description.issue | 46 | |
dc.description.openaccess | YES | |
dc.description.publisherscope | International | |
dc.description.sponsoredbyTubitakEu | N/A | |
dc.description.sponsorship | This study was funded by the Max Planck Society. Cell cartoons were created with BioRender.com. The authors thank A. Shiva for VSM measurements and TEM analysis. Open access funding enabled and organized by Projekt DEAL. | |
dc.description.version | Publisher version | |
dc.description.volume | 18 | |
dc.identifier.doi | 10.1002/smll.202204016 | |
dc.identifier.eissn | 1613-6829 | |
dc.identifier.embargo | NO | |
dc.identifier.filenameinventoryno | IR04052 | |
dc.identifier.issn | 1613-6810 | |
dc.identifier.quartile | Q1 | |
dc.identifier.scopus | 2-s2.0-85139444755 | |
dc.identifier.uri | https://doi.org/10.1002/smll.202204016 | |
dc.identifier.wos | 864413600001 | |
dc.keywords | Cancer immunotherapy | |
dc.keywords | Cell-based microrobots | |
dc.keywords | Immune cells | |
dc.keywords | immunomodulation | |
dc.keywords | Macrophages | |
dc.language.iso | eng | |
dc.publisher | Wiley | |
dc.relation.grantno | NA | |
dc.relation.ispartof | Small | |
dc.relation.uri | http://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/10930 | |
dc.subject | Science and technology | |
dc.title | Remotely guided immunobots engaged in anti-tumorigenic phenotypes for targeted cancer immunotherapy | |
dc.type | Journal Article | |
dspace.entity.type | Publication | |
local.contributor.kuauthor | Sitti, Metin | |
local.publication.orgunit1 | College of Engineering | |
local.publication.orgunit1 | SCHOOL OF MEDICINE | |
local.publication.orgunit2 | Department of Mechanical Engineering | |
local.publication.orgunit2 | School of Medicine | |
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relation.isOrgUnitOfPublication | d02929e1-2a70-44f0-ae17-7819f587bedd | |
relation.isOrgUnitOfPublication.latestForDiscovery | ba2836f3-206d-4724-918c-f598f0086a36 | |
relation.isParentOrgUnitOfPublication | 8e756b23-2d4a-4ce8-b1b3-62c794a8c164 | |
relation.isParentOrgUnitOfPublication | 17f2dc8e-6e54-4fa8-b5e0-d6415123a93e | |
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