Publication:
Remotely guided immunobots engaged in anti-tumorigenic phenotypes for targeted cancer immunotherapy

dc.contributor.coauthorDoğan, Nihal Olcay
dc.contributor.coauthorCeylan, Hakan
dc.contributor.coauthorSuadiye, Eylül
dc.contributor.coauthorSheehan, Devin
dc.contributor.coauthorAydın, Aslı
dc.contributor.coauthorYaşa, İmmihan Ceren
dc.contributor.coauthorWild, Anna-Maria
dc.contributor.coauthorRichter, Gunther
dc.contributor.departmentDepartment of Mechanical Engineering
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorSitti, Metin
dc.contributor.schoolcollegeinstituteCollege of Engineering
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T12:43:06Z
dc.date.issued2022
dc.description.abstractBuilding medical microrobots from the body's own cells may circumvent the biocompatibility concern and hence presents more potential in clinical applications to improve the possibility of escaping from the host defense mechanism. More importantly, live cells can enable therapeutically relevant functions with significantly higher efficiency than synthetic systems. Here, live immune cell-derived microrobots from macrophages, i.e., immunobots, which can be remotely steered with externally applied magnetic fields and directed toward anti-tumorigenic (M1) phenotypes, are presented. Macrophages engulf the engineered magnetic decoy bacteria, composed of 0.5 mu m diameter silica Janus particles with one side coated with anisotropic FePt magnetic nanofilm and the other side coated with bacterial lipopolysaccharide (LPS). This study demonstrates the torque-based surface rolling locomotion of the immunobots along assigned trajectories inside blood plasma, over a layer of endothelial cells, and under physiologically relevant flow rates. The immunobots secrete signature M1 cytokines, IL-12 p40, TNF-alpha, and IL-6, and M1 cell markers, CD80 and iNOS, via toll-like receptor 4 (TLR4)-mediated stimulation with bacterial LPS. The immunobots exhibit anticancer activity against urinary bladder cancer cells. This study further demonstrates such immunobots from freshly isolated primary bone marrow-derived macrophages since patient-derivable macrophages may have a strong clinical potential for future cell therapies in cancer.
dc.description.fulltextYES
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue46
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipThis study was funded by the Max Planck Society. Cell cartoons were created with BioRender.com. The authors thank A. Shiva for VSM measurements and TEM analysis. Open access funding enabled and organized by Projekt DEAL.
dc.description.versionPublisher version
dc.description.volume18
dc.identifier.doi10.1002/smll.202204016
dc.identifier.eissn1613-6829
dc.identifier.embargoNO
dc.identifier.filenameinventorynoIR04052
dc.identifier.issn1613-6810
dc.identifier.quartileQ1
dc.identifier.scopus2-s2.0-85139444755
dc.identifier.urihttps://doi.org/10.1002/smll.202204016
dc.identifier.wos864413600001
dc.keywordsCancer immunotherapy
dc.keywordsCell-based microrobots
dc.keywordsImmune cells
dc.keywordsimmunomodulation
dc.keywordsMacrophages
dc.language.isoeng
dc.publisherWiley
dc.relation.grantnoNA
dc.relation.ispartofSmall
dc.relation.urihttp://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/10930
dc.subjectScience and technology
dc.titleRemotely guided immunobots engaged in anti-tumorigenic phenotypes for targeted cancer immunotherapy
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorSitti, Metin
local.publication.orgunit1College of Engineering
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit2Department of Mechanical Engineering
local.publication.orgunit2School of Medicine
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