Publication: Adaptation of acinetobacter baumannii tocolistin exposure: laboratory mimicking of a clinical case
Program
KU-Authors
KU Authors
Co-Authors
Keske, Şiran
Publication Date
Language
Type
Embargo Status
Journal Title
Journal ISSN
Volume Title
Alternative Title
Abstract
Colistin resistance is an emerging threat. Here, we presented the development of colistin resistance in A. baumannii after colistin use on a disease course of a patient. To mimic the effects of colistin in laboratory conditions, three colistin susceptible A.baumannii isolated from the patient were exposed daily to colistin for up to 40 days. The colistin MICs and pmrABC expressions of each generation were studied. After 25 days of colistin treatment, colistin-resistant A. baumannii was isolated from the intra-abdominal fluid of the patient. During the in vitro study, the expressions of pmrCAB reached the highest level at the generation that corresponds to the duration of colistin therapy on the isolation day of the isolate. In conclusion, the duration of colistin exposure is critical for the development of colistin resistance. A. baumannii can develop a stable colistin-resistant phenotype with elevated MICs and upregulated pmrCAB operon after three weeks of colistin exposure.
Source
Publisher
DOC Design and Informatics
Subject
Colistin resistance, Kolistin direnci
Citation
Has Part
Source
Infectious Diseases and Clinical Microbiology (Online)
Book Series Title
Edition
DOI
10.36519/idcm.2020.0029