Publication:
Impact of the surface modifications and cell culture techniques on the biomechanical properties of PDMS in relation to cell growth behavior

dc.contributor.coauthorN/A
dc.contributor.departmentDepartment of Mechanical Engineering
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.departmentSchool of Medicine
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.kuauthorAydemir, Duygu
dc.contributor.kuauthorDoğru, Sedat
dc.contributor.kuauthorAlaca, Burhanettin Erdem
dc.contributor.kuauthorUlusu, Nuriye Nuray
dc.contributor.kuprofileResearcher
dc.contributor.kuprofileMaster Student
dc.contributor.kuprofileFaculty Member
dc.contributor.kuprofileFaculty Member
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteCollege of Engineering
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.contributor.schoolcollegeinstituteResearch Center
dc.contributor.yokidN/A
dc.contributor.yokidN/A
dc.contributor.yokid115108
dc.contributor.yokid6807
dc.date.accessioned2024-11-09T22:59:02Z
dc.date.issued2022
dc.description.abstractPDMS is the most widely used material in the microfluidic systems because of its advantageous properties including ease of manufacture, low cost, transparency, and gas permeability. Although all biomaterials need to be sterilized for usage for in vitro or in vivo systems to avoid possible contaminants, none of them proved effective in providing the gold standards so far for PDMS sterilization. Therefore we evaluated the influence of both UV and ethanol sterilization of PDMS substrates on the cell behavior via investigating cell proliferation, oxidative stress and nutrient metabolism of 293 T cells growing on the PDMS first time in the literature. Modulus of elasticity and stress relaxation of UV-treated PDMS samples increased compared to the ethanol-treated ones leading to a state of surface stiffness preferable for cell growth and proliferation. Oxidative stress metabolism of cells growing on the UV-treated PDMS was more stable compared to the ethanol-treated samples. Trace element and mineral metabolism are not impaired in the cells growing on the UV-treated PDMS samples. First time in the literature, we showed that UV treatment is more suitable for the PDMS samples used in the cell culture experiments. [GRAPHICS] .
dc.description.indexedbyWoS
dc.description.indexedbyScopus
dc.description.issue12
dc.description.openaccessNO
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.volume71
dc.identifier.doi10.1080/00914037.2021.1919670
dc.identifier.eissn1563-535X
dc.identifier.issn0091-4037
dc.identifier.quartileQ2
dc.identifier.scopus2-s2.0-85105505425
dc.identifier.urihttp://dx.doi.org/10.1080/00914037.2021.1919670
dc.identifier.urihttps://hdl.handle.net/20.500.14288/7827
dc.identifier.wos647863100001
dc.keywordsEthanol
dc.keywordsModulus of elasticity
dc.keywordsOxidative stress
dc.keywordsPDMS
dc.keywordsStress relaxation
dc.keywordsUV Sterilization Methods
dc.keywordsPoly (Dimethylsiloxane)
dc.keywordsIrradiation
dc.keywordsScaffolds
dc.keywordsDevices
dc.keywordsPolydimethylsiloxane
dc.keywordsMechanism
dc.keywordsTissue
dc.languageEnglish
dc.publisherTaylor & Francis
dc.relation.grantnoPresidency of Turkey, Presidency of Strategy and Budget The authors gratefully acknowledge use of the services and facilities of the KocUniversity Research Center for Translational Medicine (KUTTAM), funded by the Presidency of Turkey, Presidency of Strategy and Budget. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Presidency of Strategy and Budget.
dc.sourceInternational Journal of Polymeric Materials and Polymeric Biomaterials
dc.subjectMaterials Science, Biomaterials
dc.subjectPolymer Science
dc.titleImpact of the surface modifications and cell culture techniques on the biomechanical properties of PDMS in relation to cell growth behavior
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.authorid0000-0002-6449-2708
local.contributor.authoridN/A
local.contributor.authorid0000-0001-5931-8134
local.contributor.authorid0000-0002-3173-1389
local.contributor.kuauthorAydemir, Duygu
local.contributor.kuauthorDoğru, Sedat
local.contributor.kuauthorAlaca, Burhanettin Erdem
local.contributor.kuauthorUlusu, Nuriye Nuray
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1College of Engineering
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1Research Center
local.publication.orgunit2Department of Mechanical Engineering
local.publication.orgunit2KUTTAM (Koç University Research Center for Translational Medicine)
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Sciences and Engineering
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