Publication: CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity
Program
School / College / Institute
College of Sciences
GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
KU Authors
Co-Authors
Steib, Emmanuelle
Hamel, Virginie
Guichard, Paul
Publication Date
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Type
Embargo Status
Journal Title
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Volume Title
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Abstract
Regulation of organelle size and integrity is essential for the proper physiological functioning of eukaryotic cells. Our research spotlights CCDC15, a novel centriole inner scaffold protein, pivotal in ensuring centriole size and integrity and regulating the formation of functional cilia. Centrioles are microtubule-based organelles responsible for forming centrosomes and cilia, which serve as microtubule-organizing, signaling, and motility centers. Biogenesis and maintenance of centrioles with proper number, size, and architecture are vital for their functions during development and physiology. While centriole number control has been well-studied, less is understood about their maintenance as stable structures with conserved size and architecture during cell division and ciliary motility. Here, we identified CCDC15 as a centriole protein that colocalizes with and interacts with the inner scaffold, a crucial centriolar subcompartment for centriole size control and integrity. Using ultrastructure expansion microscopy, we found that CCDC15 depletion affects centriole length and integrity, leading to defective cilium formation, maintenance, and response to Hedgehog signaling. Moreover, loss-of-function experiments showed CCDC15's role in recruiting both the inner scaffold protein POC1B and the distal SFI1/Centrin-2 complex to centrioles. Our findings reveal players and mechanisms of centriole architectural integrity and insights into diseases linked to centriolar defects.
Source
Publisher
Rockefeller Univ Press
Subject
Cell biology
Citation
Has Part
Source
Journal of Cell Biology
Book Series Title
Edition
DOI
10.1083/jcb.202305009