Publication:
Comparison of nasopharyngeal swab and nasopharyngeal aspiration in adults for SARS-COV-2 identification using reverse transcription-polymerase chain reaction

dc.contributor.kuauthorAltuntaş, Muzaffer Ozan
dc.contributor.kuauthorGökler, Ozan
dc.contributor.kuauthorHafız, Ayşenur Meriç
dc.contributor.kuauthorÜnsaler, Selin
dc.contributor.kuauthorOkan, Ayşe
dc.contributor.kuauthorTekin, Süda
dc.contributor.kuprofileTeaching Faculty
dc.contributor.kuprofileTeaching Faculty
dc.contributor.kuprofileFaculty Member
dc.contributor.kuprofileTeaching Faculty
dc.contributor.schoolcollegeinstituteSchool of Medicine
dc.contributor.unitKoç University Hospital
dc.contributor.yokid169795
dc.contributor.yokid311179
dc.contributor.yokid53676
dc.contributor.yokid167909
dc.contributor.yokidN/A
dc.contributor.yokid42146
dc.date.accessioned2024-11-09T13:08:14Z
dc.date.issued2021
dc.description.abstractWe aimed to compare reverse transcription-polymerase chain reaction (RT-PCR) results of nasopharyngeal aspiration (NA) and nasopharyngeal swab (NS) samples in the diagnosis of coronavirus disease 2019. NS was obtained with a dacron swab and NA was performed by aspiration cannula. The sampling was performed by an otolaryngologist to ensure standardized correct sampling from the nasopharynx. RT-PCR was performed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The level of agreement between the result of NA and NS samples for each patient was analyzed. The Ct values were compared. Thirty-three patients were enrolled in the study with a mean age of 56.3 years. Thirteen subjects resulted negative with both NS and NA; 20 subjects resulted positive with NA and 18 subjects resulted positive with NS. The mean values of Ct for NA samples and NS samples were 24.6 +/- 5.9 and 24 +/- 6.7, respectively. There was no statistical difference between Ct values of NA and NS samples (p = 0.48). RT-PCR for SARS-Cov2 performed with NA sample and NS sample showed a strong correlation regarding the positivity/negativity and the Ct values.
dc.description.fulltextYES
dc.description.indexedbyWoS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue12
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipN/A
dc.description.versionPublisher version
dc.description.volume93
dc.formatpdf
dc.identifier.doi10.1002/jmv.27250
dc.identifier.eissn1096-9071
dc.identifier.embargoNO
dc.identifier.filenameinventorynoIR03044
dc.identifier.issn0146-6615
dc.identifier.linkhttps://doi.org/10.1002/jmv.27250
dc.identifier.quartileQ1
dc.identifier.scopus2-s2.0-85112606654
dc.identifier.urihttps://hdl.handle.net/20.500.14288/2672
dc.identifier.wos682523500001
dc.keywordsNasopharyngeal aspiration
dc.keywordsNasopharyngeal swab
dc.keywordsReverse transcription real-time polymerase chain reaction
dc.keywordsSARS-CoV-2
dc.languageEnglish
dc.publisherWiley
dc.relation.grantnoNA
dc.relation.urihttp://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/9703
dc.sourceJournal of Medical Virology
dc.subjectVirology
dc.titleComparison of nasopharyngeal swab and nasopharyngeal aspiration in adults for SARS-COV-2 identification using reverse transcription-polymerase chain reaction
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.authorid0000-0002-8747-7420
local.contributor.authorid0000-0003-1621-3687
local.contributor.authorid0000-0002-8003-7879
local.contributor.authorid0000-0001-7108-9194
local.contributor.authoridN/A
local.contributor.authorid0000-0001-9419-8713
local.contributor.kuauthorAltuntaş, Muzaffer Ozan
local.contributor.kuauthorGökler, Ozan
local.contributor.kuauthorHafız, Ayşenur Meriç
local.contributor.kuauthorÜnsaler, Selin
local.contributor.kuauthorOkan, Ayşe
local.contributor.kuauthorTekin, Süda

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