Publication:
Time-resolved OCT-mu PIV: a new microscopic PIV technique for noninvasive depth-resolved pulsatile flow profile acquisition

dc.contributor.coauthorChen, Chia-Yuan
dc.contributor.coauthorMenon, Prahlad G.
dc.contributor.coauthorKowalski, William
dc.contributor.departmentDepartment of Mechanical Engineering
dc.contributor.kuauthorPekkan, Kerem
dc.contributor.schoolcollegeinstituteCollege of Engineering
dc.date.accessioned2024-11-09T23:06:03Z
dc.date.issued2013
dc.description.abstractIn vivo acquisition of endothelial wall shear stress requires instantaneous depth-resolved whole-field pulsatile flow profile measurements in microcirculation. High-accuracy, quantitative and non-invasive velocimetry techniques are essential for emerging real-time mechanogenomic investigations. To address these research needs, a novel biological flow quantification technique, OCT-mu PIV, was developed utilizing high-speed optical coherence tomography (OCT) integrated with microscopic Particle Image Velocimetry (mu PIV). This technique offers the unique advantage of simultaneously acquiring blood flow profiles and vessel anatomy along arbitrarily oriented sagittal planes. The process is instantaneous and enables real-time 3D flow reconstruction without the need for computationally intensive image processing compared to state-of-the-art velocimetry techniques. To evaluate the line-scanning direction and speed, four sets of parametric synthetic OCT-mu PIV data were generated using an in-house code. Based on this investigation, an in vitro experiment was designed at the fastest scan speed while preserving the region of interest providing the depth-resolved velocity profiles spanning across the width of a micro-fabricated channel. High-agreement with the analytical flow profiles was achieved for different flow rates and seed particle types and sizes. Finally, by employing blood cells as non-invasive seeding particles, in vivo embryonic vascular velocity profiles in multiple vessels were measured in the early chick embryo. The pulsatile flow frequency and peak velocity measurements were also acquired with OCT-mu PIV, which agreed well with previous reported values. These results demonstrate the potential utility of this technique to conduct practical microfluidic and non-invasive in vivo studies for embryonic blood flows.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.issue1
dc.description.openaccessNO
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.volume54
dc.identifier.doi10.1007/s00348-012-1426-x
dc.identifier.eissn1432-1114
dc.identifier.issn0723-4864
dc.identifier.quartileQ2
dc.identifier.scopus2-s2.0-84871541630
dc.identifier.urihttps://doi.org/10.1007/s00348-012-1426-x
dc.identifier.urihttps://hdl.handle.net/20.500.14288/8910
dc.identifier.wos318153700012
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofExperiments in Fluids
dc.subjectEngineering
dc.subjectMechanical engineering
dc.subjectMechanics
dc.titleTime-resolved OCT-mu PIV: a new microscopic PIV technique for noninvasive depth-resolved pulsatile flow profile acquisition
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorPekkan, Kerem
local.publication.orgunit1College of Engineering
local.publication.orgunit2Department of Mechanical Engineering
relation.isOrgUnitOfPublicationba2836f3-206d-4724-918c-f598f0086a36
relation.isOrgUnitOfPublication.latestForDiscoveryba2836f3-206d-4724-918c-f598f0086a36
relation.isParentOrgUnitOfPublication8e756b23-2d4a-4ce8-b1b3-62c794a8c164
relation.isParentOrgUnitOfPublication.latestForDiscovery8e756b23-2d4a-4ce8-b1b3-62c794a8c164

Files