Publication:
Molecular epidemiology of bloodstream-associated Escherichia coli ST131 H30-Rx subclone infection in a region with high quinolone resistance

dc.contributor.coauthorKurt-Azap, Özlem
dc.contributor.kuauthorCan, Füsun
dc.contributor.kuauthorNurtop, Elif
dc.contributor.kuauthorİspir, Pelin
dc.contributor.kuauthorŞeref, Ceren
dc.contributor.kuauthorErgönül, Önder
dc.contributor.kuprofileFaculty Member
dc.contributor.kuprofileMaster Student
dc.contributor.kuprofileMaster Student
dc.contributor.kuprofilePhD Student
dc.contributor.kuprofileFaculty Member
dc.contributor.yokid103165
dc.contributor.yokidN/A
dc.contributor.yokidN/A
dc.contributor.yokidN/A
dc.contributor.yokid110398
dc.date.accessioned2024-11-09T22:48:50Z
dc.date.issued2016
dc.description.abstractBloodstream infections caused by Escherichia coli ST131 and ST131 H30-Rx subclones have emerged worldwide. This study was carried out to evaluate the prevalence of the ST131-Rx subclone and characterize the virulence properties of the Rx isolates among the bloodstream E. coli isolates. A total of 297 non-duplicated E. coli bloodstream isolates were studied. Antibiotic susceptibilities were tested using the disc diffusion method. PCR amplification and sequencing was used to identify ST131 and H30-Rx, the virulence gene, the beta-lactamase and virotype. Quinolone resistance among bacteraemic E. coli strains was 51 %, and it was 98% among E. coli ST131 isolates. The ST131 isolates accounted for 16% (49) of all isolates and all ST131 isolates belonged to the extraintestinal pathogenic E. coli. The proportion of H30 subclone among the ST131 isolates was 98%, and 75 % of H30 isolates belonged to the H30-Rx subclone. The prevalence of ST131 increased from 13 to 23 % in 4 years; however, there was a decrease in the ratio of H30-Rx infections. CTX-M-15 was detected in 85% of ST131 and all of the H30-Rx isolates. The virulence genes associated with adhesion, cell protection, iron uptake and toxins (papA, iha, kpsMTII, iut and sat) were more common in ST131 than in non-ST131 isolates. Most of the ST131 and H30-Rx isolates were of the C virotype. All papA-positive isolates were in virotype C. The E. coli ST131 clone has increased rapidly among bloodstream isolates. However, a decrease in the proportion of the H30-Rx subclone in the quinolone-resistant population suggests the possibility of dissemination of other virulent and quinolone-resistant subclones in hospital settings.
dc.description.indexedbyWoS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.volume65
dc.identifier.doi10.1099/jmm.0.000224
dc.identifier.eissn1473-5644
dc.identifier.issn0022-2615
dc.identifier.quartileQ3
dc.identifier.scopus2-s2.0-84962030044
dc.identifier.urihttp://dx.doi.org/10.1099/jmm.0.000224
dc.identifier.urihttps://hdl.handle.net/20.500.14288/6403
dc.identifier.wos376814600007
dc.keywordsSequence type St131
dc.keywordsClonal group
dc.keywordsClinical-features
dc.keywordsVirulence
dc.keywordsEmergence
dc.keywordsMultiresistant
dc.keywordsBacteremia
dc.keywordsUrinary
dc.languageEnglish
dc.publisherMicrobiology Society
dc.sourceJournal of Medical Microbiology
dc.subjectMicrobiology
dc.titleMolecular epidemiology of bloodstream-associated Escherichia coli ST131 H30-Rx subclone infection in a region with high quinolone resistance
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.authorid0000-0001-9387-2526
local.contributor.authorid0000-0002-9715-8394
local.contributor.authoridN/A
local.contributor.authorid0000-0002-9437-4016
local.contributor.authorid0000-0003-1935-9235
local.contributor.kuauthorCan, Füsun
local.contributor.kuauthorNurtop, Elif
local.contributor.kuauthorİspir, Pelin
local.contributor.kuauthorŞeref, Ceren
local.contributor.kuauthorErgönül, Mehmet Önder
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1Graduate School of Health Sciences
local.publication.orgunit2School of Medicine

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