Publication:
Evaluation of the antimicrobial activity of triple enzyme-embedded organic-inorganic Hybrid Nanoflowers (hNFs) in comparison with powerful antimicrobial agent chitosan

dc.contributor.coauthorCakir, Seda
dc.contributor.coauthorOzdemir, Nalan
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.departmentGraduate School of Health Sciences
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorAydemir, Duygu
dc.contributor.kuauthorUlusu, Nuriye Nuray
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF HEALTH SCIENCES
dc.contributor.schoolcollegeinstituteResearch Center
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2025-03-06T20:57:53Z
dc.date.issued2024
dc.description.abstractOrganic-inorganic hybrid nanoflowers (hNFs) have high stability, reusability, low production cost, and overcome substrate/product inhibition. Antimicrobial activity of various hNFs has been reported to overcome environmental microbial contaminations and infections, which are considered major public health problems. alpha-amylase, protease, and lipase are the most common industrial enzymes exerting antimicrobial activity;therefore, we synthesized triple enzyme (alpha-amylase, protease, and lipase)-embedded hNFs by using pancreatin to evaluate their antimicrobial activity in comparison with one of the most potent antimicrobial polymer chitosan. The broad spectrum of the antimicrobial properties of chitosan is used in industrial products, including cosmetics, food, agriculture, pharmaceuticals, and textiles. SEM analysis, thermogravimetric analysis (TGA), and the degree of deacetylation (%DD) were performed for chitosan characterization, where SEM, FTIR, EDX, and XRD analyses were performed for the characterization of hNFs. The catalytic activity of pancreatin and hNFs was evaluated by measuring lipase, alpha-amylase, and protease enzyme activities at 37 degrees C. Antibacterial activities of hNFs, pancreatin, and chitosan were tested on gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria, compared to the pancreatin and chitosan via agar and broth dilution methods. hNFs showed enhanced catalytic activity for protease, lipase, and alpha-amylase compared to pancreatin at different pH values (pH 8, 9). hNFs showed catalytic activity after being washed and reused up to six times, indicating their reusability and recoverability. hNFs showed significant antimicrobial activity, such as chitosan, Staphylococcus aureus, and Escherichia coli, compared to pancreatin. Our novel hNFs can be used to develop antimicrobial technologies to fight against environmental microbial contaminations and antibiotic resistance-driven environmental pathogens.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipThe authors gratefully acknowledge the use of the services and facilities of the Koc University Research Center for Translational Medicine (KUTTAM), funded by the Presidency of Turkey, Presidency of Strategy and Budget. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Presidency of Strategy and Budget.
dc.identifier.doi10.1007/s00284-024-03884-6
dc.identifier.eissn1432-0991
dc.identifier.grantnoPresidency of Turkey, Presidency of Strategy and Budget
dc.identifier.issn0343-8651
dc.identifier.issue11
dc.identifier.quartileQ3
dc.identifier.scopus2-s2.0-85204286822
dc.identifier.urihttps://doi.org/10.1007/s00284-024-03884-6
dc.identifier.urihttps://hdl.handle.net/20.500.14288/27341
dc.identifier.volume81
dc.identifier.wos1314982900001
dc.keywordsOrganic-inorganic hybrid nanoflowers
dc.keywordsAntimicrobial activity
dc.keywordsAlpha-amylase
dc.keywordsProtease
dc.keywordsLipase
dc.keywordsPancreatin
dc.keywordsChitosan
dc.keywordsMicrobial contamination
dc.keywordsEnzyme-embedded nanoflowers
dc.keywordsReusability
dc.keywordsCatalytic activity
dc.keywordsStaphylococcus aureus
dc.keywordsEscherichia coli
dc.keywordsAntibiotic resistance
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofCURRENT MICROBIOLOGY
dc.subjectMicrobiology
dc.titleEvaluation of the antimicrobial activity of triple enzyme-embedded organic-inorganic Hybrid Nanoflowers (hNFs) in comparison with powerful antimicrobial agent chitosan
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorAydemir, Duygu
local.contributor.kuauthorUlusu, Nuriye Nuray
local.publication.orgunit1GRADUATE SCHOOL OF HEALTH SCIENCES
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1Research Center
local.publication.orgunit2KUTTAM (Koç University Research Center for Translational Medicine)
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Health Sciences
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