Publication:
The importance of histological patterns on PD-L1 staining heterogeneity: should we use pattern-based approach for selecting tumor samples for PD-L1 testing in lung adenocarcinomas?

dc.contributor.coauthorZeren, Handan
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorBulutay, Pınar
dc.contributor.kuauthorErus, Suat
dc.contributor.kuauthorFırat, Pınar Arıkan
dc.contributor.kuauthorTanju, Serhan
dc.contributor.kuauthorDilege, Şükrü
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T13:19:07Z
dc.date.issued2021
dc.description.abstractBackground/aim: programmed death ligand-1 (PD-L1) is a predictive marker for immunotherapeutic agents. However, heterogeneous staining of PD-L1 can cause false-negative results. The aim of this study is to evaluate the importance of histological patterns on PD-L1 staining heterogeneity in lung adenocarcinomas (LAC). Materials and methods: PD-L1 immunohistochemistry (IHC) stain was performed to two different tissue cores of 128 LAC cases, and cut-off values are given for grouping the cases according to the percentage of staining (1%-10%, 11%-49%, 50%-100%). Staining rates between cores were compared and analyzed by their histological patterns. Also, the relation of the PD-L1 expression with the clinicopathological characteristics of the cases was analyzed. Results: overall, PD-L1 expression was observed in 53 of 128 cases (41.4%, 1% cut-off), 23.5% of them were positive at 10% cut-off and 14.1% at 50% cut-off. PD-L1 expression was significantly related to the high grade micropapillary and solid patterns of adenocarcinomas (p:0.01). Staining cut-offs were mostly similar between cores (43/50, 86%) (k:0.843). However, 14% of them were positive only in one core (7 of 50). This false negativity was mostly related to the histological patterns. Conclusion: our data reveal the heterogeneous staining of PD-L1 expression, also micropapillary and solid patterns show higher rates of PDL expression. Therewithal, these findings also highlight the importance of taking into consideration of histological patterns, when choosing a paraffin block for the PDL1.
dc.description.fulltextYES
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue1
dc.description.openaccessYES
dc.description.publisherscopeNational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipN/A
dc.description.versionPublisher version
dc.description.volume51
dc.identifier.doi10.3906/sag-2004-61
dc.identifier.eissn1303-6165
dc.identifier.embargoNO
dc.identifier.filenameinventorynoIR02723
dc.identifier.issn1300-0144
dc.identifier.quartileQ3
dc.identifier.scopus2-s2.0-85099244422
dc.identifier.urihttps://hdl.handle.net/20.500.14288/3083
dc.identifier.wos623196400026
dc.keywordsPD-L1
dc.keywordsLung cancer
dc.keywordsImmunotherapy
dc.keywordsLung adenocarcinoma
dc.language.isoeng
dc.publisherTÜBİTAK
dc.relation.grantnoNA
dc.relation.ispartofTurkish Journal of Medical Sciences
dc.relation.urihttp://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/9369
dc.subjectMedicine
dc.subjectGeneral and internal medicine
dc.titleThe importance of histological patterns on PD-L1 staining heterogeneity: should we use pattern-based approach for selecting tumor samples for PD-L1 testing in lung adenocarcinomas?
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorBulutay, Pınar
local.contributor.kuauthorFırat, Pınar Arıkan
local.contributor.kuauthorErus, Suat
local.contributor.kuauthorTanju, Serhan
local.contributor.kuauthorDilege, Mustafa Şükrü
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit2School of Medicine
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relation.isOrgUnitOfPublication.latestForDiscoveryd02929e1-2a70-44f0-ae17-7819f587bedd
relation.isParentOrgUnitOfPublication17f2dc8e-6e54-4fa8-b5e0-d6415123a93e
relation.isParentOrgUnitOfPublication.latestForDiscovery17f2dc8e-6e54-4fa8-b5e0-d6415123a93e

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