Publication:
Hsp90/hsp70 chaperone machine regulation of the saccharomyces mal-activator as determined in vivo using noninducible and constitutive mutant alleles

dc.contributor.coauthorRan, Fulai
dc.contributor.coauthorMichels, Corinne A.
dc.date.accessioned2024-11-09T23:43:44Z
dc.date.issued2008
dc.description.abstractThe Hsp90/Hsp70 chaperone machine is an essential regulator of cell growth and division. It is required for activation of select client proteins, chiefly protein kinases and transcription activators and thus plays a major role in regulating intracellular signaling and gene expression. This report demonstrates, in vivo, the association of the Saccharomyces cerevisiae maltose-responsive transcription activator Mal63 (MAL-activator) with the yeast Hsp70 (Ssa1), Hsp90 (Hsp82), and Hop (Sti1) homologs, using a collection of inducible, constitutive, and noninducible alleles. Each class of mutant activator forms a distinctly different stable multichaperone complex in the absence of maltose. Inducible Mal63p associates with Ssa1, Hsp82, and Sti1 and is released in the presence of maltose. Noninducible mal63 mutant proteins bind to Ssa1 alone and do not stably associate with Hsp82 or Sti1. ConstitutiveMAL-activators bind well to Hsp82 and poorly to Ssa1 and Sti1, but deletion of STI1 restores Ssa1 binding. Taken together, Mal63p regulation requires the formation of Hsp90/Hsp70 subcomplexes comparable to, yet distinct from those observed with previously characterized Hsp90 clients including glucocorticoid receptor and yeast Hap1p. Thus, comparative studies of different client proteins highlight functional diversity in the operation of the Hsp90/Hsp70 chaperone machine. Copyright © 2008 by the Genetics Society of America.
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue1
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.volume179
dc.identifier.doi10.1534/genetics.107.084921
dc.identifier.issn0016-6731
dc.identifier.quartileQ1
dc.identifier.scopus2-s2.0-52049091534
dc.identifier.urihttps://doi.org/10.1534/genetics.107.084921
dc.identifier.urihttps://hdl.handle.net/20.500.14288/13545
dc.keywordsAlpha glucosidase
dc.keywordsFungal protein
dc.keywordsHeat shock protein
dc.keywordsHeat shock protein 70
dc.keywordsHeat shock protein 82
dc.keywordsHeat shock protein 90
dc.keywordsMaltose
dc.keywordsProtein ssa1
dc.keywordsProtein sti1
dc.keywordsProtein stil1
dc.keywordsRo antibody
dc.keywordsUnclassified drug
dc.keywordsChaperone
dc.keywordsHeat shock protein 70
dc.keywordsHeat shock protein 90
dc.keywordsHSP82 protein, S cerevisiae
dc.keywordsMAL63 protein, S cerevisiae
dc.keywordsMaltose
dc.keywordsPrimer DNA
dc.keywordsSaccharomyces cerevisiae protein
dc.keywordsSSA3 protein, S cerevisiae
dc.keywordsTranscription factor
dc.keywordsAllele
dc.keywordsArticle
dc.keywordsControlled study
dc.keywordsFungal strain
dc.keywordsGene control
dc.keywordsGene deletion
dc.keywordsImmunoprecipitation
dc.keywordsIn vivo study
dc.keywordsNonhuman
dc.keywordsPriority journal
dc.keywordsProtein expression
dc.keywordsSaccharomyces cerevisiae
dc.keywordsComparative study
dc.keywordsGene expression regulation
dc.keywordsGenetics
dc.keywordsImmunoblotting
dc.keywordsMetabolism
dc.keywordsMutation
dc.keywordsSaccharomyces
dc.keywordsSaccharomyces cerevisiae
dc.keywordsDNA primers
dc.keywordsGene expression regulation, fungal
dc.keywordsHSP70 heat-shock proteins
dc.keywordsHSP90 heat-ahock proteins
dc.keywordsImmunoblotting
dc.keywordsImmunoprecipitation
dc.keywordsMaltose
dc.keywordsMolecular chaperones
dc.keywordsMutation
dc.keywordsSaccharomyces cerevisiae
dc.keywordsSaccharomyces cerevisiae proteins
dc.keywordsTranscription factors
dc.language.isoeng
dc.publisherGenetics Society America (GSA)
dc.relation.ispartofGenetics
dc.subjectGenetic
dc.titleHsp90/hsp70 chaperone machine regulation of the saccharomyces mal-activator as determined in vivo using noninducible and constitutive mutant alleles
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorBali, Mehtap

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