Publication:
Dependence of erythrocyte deformability on mechanical stress and oxygenation

dc.contributor.departmentDepartment of Physics
dc.contributor.departmentGraduate School of Health Sciences
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorAksu, Ali Cenk
dc.contributor.kuauthorErten, Ahmet Can
dc.contributor.kuauthorSağlam, Gökay
dc.contributor.kuauthorUğurel, Elif
dc.contributor.kuauthorYalçın, Özlem
dc.contributor.schoolcollegeinstituteCollege of Engineering
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF HEALTH SCIENCES
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T23:10:42Z
dc.date.issued2017
dc.description.abstractMechanical properties of erythrocytes are known to be affected by their oxygenation status. Several studies suggested that cytoskeletal rearrangements are carried out in an oxygen dependent manner. The structure of the cytoskeleton determines the mechanical properties of erythrocyte membrane. However, oxygen-dependent mechanical characteristics of erythrocyte are poorly studied whether oxygenated state could alter erythrocyte deformability. In this study, we investigated shear stress induced improvements in erythrocyte deformability through their oxygenation status. Venous blood was collected from male, healthy volunteers (n=10) between 25–50 ages. An informed written consent was obtained from each subject participated in the study according to Declaration of Helsinki. The hematocrit of blood samples adjusted to 0.4 l/l with autologous plasma. Whole blood samples were diluted with polyvinylpyrrolidone (PVP) solution (Mechatronics, Hoorn, Netherlands) with a dilution ratio of 1/200. Blood samples were equilibrated with either ambient air or nitrogen gas for at least 10 minutes at room temperature. Erythrocyte deformability was measured by a laser-assisted optical rotational cell analyzer (LORRCA MaxSis, Mechatronics, Netherlands) applying shear stresses (SS) ranging between 0.3 to 50 Pa. Then, a constant SS of 5, 10 and 20 Pa were applied continuously for 300 seconds and erythrocyte deformability was measured immediately afterwards. Maximal erythrocyte elongation index (EImax) and the SS required for one-half of this maximal deformation (SS1/2) were calculated by using the linear Lineweaver-Burke (LB) model. Deoxygenation of blood samples significantly decreased SS1/2 values both before and after SS applications (p < 0.001). EImax was significantly increased in deoxygenated blood before applying 5 Pa SS (p < 0.05). However, there were no significant differences after continuous SS in oxygenated and deoxygenated blood. Deoxygenation significantly decreased SS1/2/EImax values both before and after SS applications (p < 0.01). SS1/2/EImax values in both oxygenated and deoxygenated blood were significantly decreased after 5 and 10 Pa continuous SS applications although they were not significantly decreased after applying 20 Pa SS. Our study showed for the first time that erythrocyte deformability is improved in deoxygenated conditions in contrast to results presented in previous studies. This deformability improvement may control blood flow and consequently erythrocyte distribution within hypoxic tissues. Our study also demonstrated the relationship of oxygenation-deoxygenation shifts and magnitude of shear stress on erythrocyte deformability.
dc.description.indexedbyWOS
dc.description.openaccessNO
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuTÜBİTAK
dc.description.sponsorshipScientific and Technological Research Council of Turkey [214S186] This study was supported by the Scientific and Technological Research Council of Turkey Grant 214S186.
dc.description.volume31
dc.identifier.eissn1530-6860
dc.identifier.issn0892-6638
dc.identifier.quartileQ1
dc.identifier.urihttps://hdl.handle.net/20.500.14288/9522
dc.identifier.wos405986504297
dc.keywordsBiochemistry
dc.keywordsBiology
dc.keywordsCell biology
dc.language.isoeng
dc.publisherFederation amer Soc Exp Biol
dc.relation.ispartofFaseb Journal
dc.subjectBiochemistry
dc.subjectMolecular biology
dc.subjectBiology
dc.subjectCell biology
dc.titleDependence of erythrocyte deformability on mechanical stress and oxygenation
dc.typeMeeting Abstract
dspace.entity.typePublication
local.contributor.kuauthorYalçın, Özlem
local.contributor.kuauthorUğurel, Elif
local.contributor.kuauthorSağlam, Gökay
local.contributor.kuauthorErten, Ahmet Can
local.contributor.kuauthorAksu, Ali Cenk
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1College of Engineering
local.publication.orgunit1GRADUATE SCHOOL OF HEALTH SCIENCES
local.publication.orgunit1College of Sciences
local.publication.orgunit2Department of Physics
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Health Sciences
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