Publication:
Bacterial species and total bacterial load in the distal oesophagus in patients with and without clinical gastric reflux

dc.contributor.coauthorKayar Dogan E.
dc.contributor.coauthorAbaci Gunyar O.
dc.contributor.coauthorTopal F.
dc.contributor.coauthorEkinci N.
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorAlper, Emrah
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-10T00:10:25Z
dc.date.issued2019
dc.description.abstractAims: The purpose of this study is to compare distal oesophagus of persons with and without gastric reflux in terms of bacterial load and presence of certain bacterial species. Methods and Results: Two biopsy specimens were obtained from the distal oesophagus at 5 cm above the gastroesophageal junction of each of the 50 patients (20 with normal oesophagus and 30 with reflux oesophagitis) under endoscopic examination and used for histological examination and DNA isolation. We used a real-time PCR-based assay to quantify the bacterial load and the presence of certain bacterial species from one of the biopsy samples. The biopsy specimens taken from the patients with reflux oesophagitis were consistent with gastroesophageal reflux disease (GERD). The bacterial load did not significantly differ between the groups (P < 0·005). Conclusion: While there was no difference between the bacterial load in the two groups, variation was observed in bacterial species. Most of the bacteria identified in distal oesophagus of the patients with gastroesophageal reflux were Gram negative. Significance and Impact of the Study: The human oesophagus was considered sterile until quite recently. Molecular techniques displayed the presence of a diverse bacterial species in the oesophagus. Although it is known that dysbiosis in the oesophagus causes GERD, and that Barrett's oesophagus can trigger the development of oesophageal adenocarcinoma, its etiopathogenesis is not clear. A limited number of published studies support the importance of the present study.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue6
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.volume126
dc.identifier.doi10.1111/jam.14250
dc.identifier.issn1364-5072
dc.identifier.scopus2-s2.0-85064704824
dc.identifier.urihttps://doi.org/10.1111/jam.14250
dc.identifier.urihttps://hdl.handle.net/20.500.14288/17296
dc.keywordsBacterial load
dc.keywordsBacterial species
dc.keywordsDistal oesophagus
dc.keywordsGastric reflux
dc.keywordsReal-time PCR
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofJournal of Applied Microbiology
dc.subjectBiotechnology
dc.subjectApplied microbiology
dc.subjectMicrobiology
dc.titleBacterial species and total bacterial load in the distal oesophagus in patients with and without clinical gastric reflux
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorAlper, Emrah
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit2School of Medicine
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