Publication:
A proximity mapping journey into the biology of the mammalian centrosome/cilium complex

dc.contributor.departmentDepartment of Molecular Biology and Genetics
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.kuauthorArslanhan, Melis Dilara
dc.contributor.kuauthorGülensoy, Dila
dc.contributor.kuauthorKaralar, Elif Nur Fırat
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.date.accessioned2024-11-09T13:52:48Z
dc.date.issued2020
dc.description.abstractThe mammalian centrosome/cilium complex is composed of the centrosome, the primary cilium and the centriolar satellites, which together regulate cell polarity, signaling, proliferation and motility in cells and thereby development and homeostasis in organisms. Accordingly, deregulation of its structure and functions is implicated in various human diseases including cancer, developmental disorders and neurodegenerative diseases. To better understand these disease connections, the molecular underpinnings of the assembly, maintenance and dynamic adaptations of the centrosome/cilium complex need to be uncovered with exquisite detail. Application of proximity-based labeling methods to the centrosome/cilium complex generated spatial and temporal interaction maps for its components and provided key insights into these questions. In this review, we first describe the structure and cell cycle-linked regulation of the centrosome/cilium complex. Next, we explain the inherent biochemical and temporal limitations in probing the structure and function of the centrosome/cilium complex and describe how proximity-based labeling approaches have addressed them. Finally, we explore current insights into the knowledge we gained from the proximity mapping studies as it pertains to centrosome and cilium biogenesis and systematic characterization of the centrosome, cilium and centriolar satellite interactomes.
dc.description.fulltextYES
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue6
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuEU - TÜBİTAK
dc.description.sponsorshipH2020 European Research Council Starting Grant
dc.description.sponsorshipEuropean Union (European Union)
dc.description.sponsorshipHorizon 2020
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TÜBİTAK)
dc.description.sponsorshipEuropean Molecular Biology Organization (EMBO) Installation Grant
dc.description.sponsorshipEMBO Young Investigator Award
dc.description.sponsorshipBAGEP Young Investigator Award
dc.description.versionPublisher version
dc.description.volume9
dc.identifier.doi10.3390/cells9061390
dc.identifier.embargoNO
dc.identifier.filenameinventorynoIR02265
dc.identifier.issn2073-4409
dc.identifier.quartileN/A
dc.identifier.scopus2-s2.0-85086050989
dc.identifier.urihttps://doi.org/10.3390/cells9061390
dc.identifier.wos552493500001
dc.keywordsAPEX
dc.keywordsBioID
dc.keywordsCentriolar satellites
dc.keywordsCentrosome
dc.keywordsCilia
dc.keywordsCiliopathies
dc.keywordsMicrotubules
dc.keywordsProximity-labeling
dc.keywordsTurboID
dc.language.isoeng
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.relation.grantno679140
dc.relation.grantno214Z223
dc.relation.ispartofCells
dc.relation.urihttp://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/8906
dc.subjectCell biology
dc.titleA proximity mapping journey into the biology of the mammalian centrosome/cilium complex
dc.typeReview
dspace.entity.typePublication
local.contributor.kuauthorArslanhan, Melis Dilara
local.contributor.kuauthorGülensoy, Dila
local.contributor.kuauthorKaralar, Elif Nur Fırat
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1College of Sciences
local.publication.orgunit2Department of Molecular Biology and Genetics
local.publication.orgunit2Graduate School of Sciences and Engineering
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