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Increased expression of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-3 is required for growth of mouse embryonic stem cells that are undergoing differentiation

dc.contributor.coauthorGüzel, Saime
dc.contributor.coauthorAltunok, Tuğba Hazal
dc.contributor.coauthorYalçın, Abdullah
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.kuauthorGürpınar, Yunus
dc.contributor.schoolcollegeinstituteResearch Center
dc.date.accessioned2024-11-09T23:22:00Z
dc.date.issued2023
dc.description.abstractThe unlimited proliferation capacity of embryonic stem cells (ESCs) coupled with their capability to differentiate into several cell types makes them an attractive candidate for studying the molecular mechanisms regulating self-renewal and transition from pluripotent state. Although the roles of 6-phosphofructo-2-kinase/fructose-2,6-bisphos-phatase family (PFKFB1-4) in cell survival, proliferation, and differentiation in tumor cells have been studied, their role in mouse ESC (mESC) biology is currently unkown. In the current study, Pfkfb isoenzyme expressions were analyzed in R1 and J1 mESCs that were cultured in the presence and absence of leukemia inhibitory factor (LIF). We report that expression of the Pfkfb3 isoenzyme was markedly increased when mESCs were promoted to differentiate upon LIF removal. We then demonstrated that Pfkfb3 silencing induced the differentiation marker Brachyury suggesting that Pfkfb3 may be required for the regulation of mesodermal differentiation of mESCs. Furthermore, we show that the increase in Pfkfb3 expression is required for the growth of early differentiated mESCs. Although these results provide important insights into the early differentiation of mESCs with regard to Pfkfb expressions, further mechanistic studies will be needed for understanding the pathways and mechanisms involved in regulation of proliferation and early differentiation of mESCs through Pfkfb3.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue1
dc.description.openaccessNO
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipScientific Research Fund of Bursa Uludag University [OUAP(V)-2013/27]
dc.description.sponsorshipScientific and Technological Research Council of Turkey [116Z570] This work was supported by the Scientific Research Fund of Bursa Uludag University [grant number# OUAP(V)-2013/27] and Scientific and Technological Research Council of Turkey [grant number#116Z570).
dc.description.volume75
dc.identifier.doi10.1007/s10616-022-00557-9
dc.identifier.eissn1573-0778
dc.identifier.issn0920-9069
dc.identifier.quartileQ4
dc.identifier.scopus2-s2.0-85141556547
dc.identifier.urihttps://doi.org/10.1007/s10616-022-00557-9
dc.identifier.urihttps://hdl.handle.net/20.500.14288/10974
dc.identifier.wos880554400001
dc.keywordsMouse embryonic stem cells
dc.keywords6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase-3
dc.keywordsBrachyury
dc.keywordsLeukemia inhibitory factor PFKFB3
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofCytotechnology
dc.subjectBiotechnology
dc.subjectApplied microbiology
dc.subjectCell biology
dc.titleIncreased expression of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-3 is required for growth of mouse embryonic stem cells that are undergoing differentiation
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorGürpınar, Yunus
local.publication.orgunit1Research Center
local.publication.orgunit2KUTTAM (Koç University Research Center for Translational Medicine)
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relation.isOrgUnitOfPublication.latestForDiscovery91bbe15d-017f-446b-b102-ce755523d939
relation.isParentOrgUnitOfPublicationd437580f-9309-4ecb-864a-4af58309d287
relation.isParentOrgUnitOfPublication.latestForDiscoveryd437580f-9309-4ecb-864a-4af58309d287

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