Publication: Understanding lipid metabolism in high-lipid-producing chlorella vulgaris mutants at the genome-wide level
dc.contributor.coauthor | Ünlü, Yiğit Sabri | |
dc.contributor.coauthor | Cevahir, Gül | |
dc.contributor.department | Department of Chemical and Biological Engineering | |
dc.contributor.department | Graduate School of Sciences and Engineering | |
dc.contributor.department | KUTEM (Koç University Tüpraş Energy Center) | |
dc.contributor.kuauthor | Erkey, Can | |
dc.contributor.kuauthor | Kavaklı, İbrahim Halil | |
dc.contributor.kuauthor | Sarayloo, Ehsan | |
dc.contributor.kuauthor | Şimşek, Salim | |
dc.contributor.kuauthor | Tardu, Mehmet | |
dc.contributor.schoolcollegeinstitute | College of Engineering | |
dc.contributor.schoolcollegeinstitute | GRADUATE SCHOOL OF SCIENCES AND ENGINEERING | |
dc.contributor.schoolcollegeinstitute | Research Center | |
dc.date.accessioned | 2024-11-09T23:44:15Z | |
dc.date.issued | 2017 | |
dc.description.abstract | In this study, physical and chemical mutagenesis methods were applied to enhance lipid productivity in Chlorella vulgaris. Then, de novo RNA-seq was performed to observe lipid metabolism changes at the genome-wide level. Characterization of two mutants, UV-715 and EMS-25, showed marked increases in lipid contents, i.e., 42% and 45%, respectively. In addition, the biomass productivity of the UV-715 cells was 9% higher than that of wildtype cells. Furthermore, gas chromatography-mass spectrophotometry analysis showed that both mutants have higher fatty acid methyl ester (FAME) contents than wild-type cells. To understand the effect of mutations that caused yield changes in UV-715 and EMS-25 cells at a genome-wide level, we carried out de novo RNA-seq. As expected, the transcriptional levels of the lipid biosynthesis genes were up-regulated, while the transcriptional levels of genes involved in lipid catabolism were down-regulated. Surprisingly, the transcriptional levels of the genes involved in nitrate assimilation and detoxification of reactive oxygen species (ROS) were significantly increased in the mutants. The genome-wide analysis results highlight the importance of nitrate metabolism and detoxification of ROS for high biomass and lipid productivity. | |
dc.description.indexedby | WOS | |
dc.description.indexedby | Scopus | |
dc.description.openaccess | NO | |
dc.description.sponsoredbyTubitakEu | N/A | |
dc.description.sponsorship | Istanbul Development Agency [ISTKA-TR/14/EVK/0039] | |
dc.description.sponsorship | TUPRAS Energy Research Center [0032] | |
dc.description.sponsorship | Istanbul University BAP [39324] This work was supported by an Istanbul Development Agency grant (ISTKA-TR/14/EVK/0039) | |
dc.description.sponsorship | TUPRAS Energy Research Center (0032) | |
dc.description.sponsorship | Istanbul University BAP project 39324. | |
dc.description.volume | 28 | |
dc.identifier.doi | 10.1016/j.algal.2017.11.009 | |
dc.identifier.issn | 2211-9264 | |
dc.identifier.scopus | 2-s2.0-85033502527 | |
dc.identifier.uri | https://doi.org/10.1016/j.algal.2017.11.009 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14288/13633 | |
dc.identifier.wos | 415840400028 | |
dc.keywords | Chlorella vulgaris | |
dc.keywords | De novo RNA-seq | |
dc.keywords | Biodiesel | |
dc.keywords | Lipid biosynthesis | |
dc.keywords | Mutagenesis | |
dc.keywords | Fatty-acid-composition | |
dc.keywords | Superoxide dismutases | |
dc.keywords | Siroheme biosynthesis | |
dc.keywords | Epoxide hydrolases | |
dc.keywords | Random mutagenesis | |
dc.keywords | Stress-response | |
dc.keywords | Cell-growth | |
dc.keywords | Microalgae | |
dc.keywords | Genes | |
dc.keywords | Nannochloropsis | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.relation.ispartof | Algal Research-Biomass Biofuels And Bioproducts | |
dc.subject | Biotechnology | |
dc.subject | Microbiology | |
dc.title | Understanding lipid metabolism in high-lipid-producing chlorella vulgaris mutants at the genome-wide level | |
dc.type | Journal Article | |
dspace.entity.type | Publication | |
local.contributor.kuauthor | Sarayloo, Ehsan | |
local.contributor.kuauthor | Tardu, Mehmet | |
local.contributor.kuauthor | Şimşek, Salim | |
local.contributor.kuauthor | Erkey, Can | |
local.contributor.kuauthor | Kavaklı, İbrahim Halil | |
local.publication.orgunit1 | GRADUATE SCHOOL OF SCIENCES AND ENGINEERING | |
local.publication.orgunit1 | College of Engineering | |
local.publication.orgunit1 | Research Center | |
local.publication.orgunit2 | Department of Chemical and Biological Engineering | |
local.publication.orgunit2 | KUTEM (Koç University Tüpraş Energy Center) | |
local.publication.orgunit2 | Graduate School of Sciences and Engineering | |
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