Publication:
Understanding lipid metabolism in high-lipid-producing chlorella vulgaris mutants at the genome-wide level

dc.contributor.coauthorÜnlü, Yiğit Sabri
dc.contributor.coauthorCevahir, Gül
dc.contributor.departmentDepartment of Chemical and Biological Engineering
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.departmentKUTEM (Koç University Tüpraş Energy Center)
dc.contributor.kuauthorErkey, Can
dc.contributor.kuauthorKavaklı, İbrahim Halil
dc.contributor.kuauthorSarayloo, Ehsan
dc.contributor.kuauthorŞimşek, Salim
dc.contributor.kuauthorTardu, Mehmet
dc.contributor.schoolcollegeinstituteCollege of Engineering
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteResearch Center
dc.date.accessioned2024-11-09T23:44:15Z
dc.date.issued2017
dc.description.abstractIn this study, physical and chemical mutagenesis methods were applied to enhance lipid productivity in Chlorella vulgaris. Then, de novo RNA-seq was performed to observe lipid metabolism changes at the genome-wide level. Characterization of two mutants, UV-715 and EMS-25, showed marked increases in lipid contents, i.e., 42% and 45%, respectively. In addition, the biomass productivity of the UV-715 cells was 9% higher than that of wildtype cells. Furthermore, gas chromatography-mass spectrophotometry analysis showed that both mutants have higher fatty acid methyl ester (FAME) contents than wild-type cells. To understand the effect of mutations that caused yield changes in UV-715 and EMS-25 cells at a genome-wide level, we carried out de novo RNA-seq. As expected, the transcriptional levels of the lipid biosynthesis genes were up-regulated, while the transcriptional levels of genes involved in lipid catabolism were down-regulated. Surprisingly, the transcriptional levels of the genes involved in nitrate assimilation and detoxification of reactive oxygen species (ROS) were significantly increased in the mutants. The genome-wide analysis results highlight the importance of nitrate metabolism and detoxification of ROS for high biomass and lipid productivity.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.openaccessNO
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipIstanbul Development Agency [ISTKA-TR/14/EVK/0039]
dc.description.sponsorshipTUPRAS Energy Research Center [0032]
dc.description.sponsorshipIstanbul University BAP [39324] This work was supported by an Istanbul Development Agency grant (ISTKA-TR/14/EVK/0039)
dc.description.sponsorshipTUPRAS Energy Research Center (0032)
dc.description.sponsorshipIstanbul University BAP project 39324.
dc.description.volume28
dc.identifier.doi10.1016/j.algal.2017.11.009
dc.identifier.issn2211-9264
dc.identifier.scopus2-s2.0-85033502527
dc.identifier.urihttps://doi.org/10.1016/j.algal.2017.11.009
dc.identifier.urihttps://hdl.handle.net/20.500.14288/13633
dc.identifier.wos415840400028
dc.keywordsChlorella vulgaris
dc.keywordsDe novo RNA-seq
dc.keywordsBiodiesel
dc.keywordsLipid biosynthesis
dc.keywordsMutagenesis
dc.keywordsFatty-acid-composition
dc.keywordsSuperoxide dismutases
dc.keywordsSiroheme biosynthesis
dc.keywordsEpoxide hydrolases
dc.keywordsRandom mutagenesis
dc.keywordsStress-response
dc.keywordsCell-growth
dc.keywordsMicroalgae
dc.keywordsGenes
dc.keywordsNannochloropsis
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofAlgal Research-Biomass Biofuels And Bioproducts
dc.subjectBiotechnology
dc.subjectMicrobiology
dc.titleUnderstanding lipid metabolism in high-lipid-producing chlorella vulgaris mutants at the genome-wide level
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorSarayloo, Ehsan
local.contributor.kuauthorTardu, Mehmet
local.contributor.kuauthorŞimşek, Salim
local.contributor.kuauthorErkey, Can
local.contributor.kuauthorKavaklı, İbrahim Halil
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1College of Engineering
local.publication.orgunit1Research Center
local.publication.orgunit2Department of Chemical and Biological Engineering
local.publication.orgunit2KUTEM (Koç University Tüpraş Energy Center)
local.publication.orgunit2Graduate School of Sciences and Engineering
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