Publication:
Data reduction methods for ektacytometry in clinical hemorheology

dc.contributor.coauthorMeiselman, Herbert J.
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorBaşkurt, Oğuz Kerim
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T13:27:23Z
dc.date.issued2013
dc.description.abstractLaser-diffraction ektacytometry is a generally accepted technique for measuring RBC deformability induced by fluid shear stress (SS) and yields paired elongation index-SS data at several levels of stress. Unfortunately, comparison of results is hindered by the lack of simple indices that accurately characterize these data. Several mathematical models have been proposed, including those developed for analysis of enzyme kinetics (Lineweaver-Burk, Eadie-Hofstee) and curve fitting (Streekstra-Bronkhorst). All of these analytical approaches provide a value for cell deformation at infinite stress (EImax) and the shear stress required to achieve one-half of this deformation (SS1/2); the use of non-linear regression is essential when calculating these parameters. While the current models provide equivalent results for normal RBC if used with non-linear regression, EImax and SS1/2 are not always concordant for cells with abnormal mechanical behavior. This technical note examines such differences for three conditions: glutaraldehyde treatment, mechanical stress and non-isotonic media. It was found that none of the models yield completely satisfactory values for EImax and SS1/2, especially if there are large changes of EImax. However, the ratio of SS1/2 to EImax (SS1/2/EImax) is much less affected by these problems, has similar power (i.e., standardized difference) as SS1/2 and EImax and is more robust in reflecting alterations of deformability. We thus conclude that the SS1/2/EImax ratio can be used when reporting and comparing various populations of RBC or cells obtained from subjects having different clinical states.
dc.description.fulltextYES
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue1
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuEU - TÜBİTAK
dc.description.sponsorshipN/A
dc.description.versionPublisher version
dc.description.volume54
dc.identifier.doi10.3233/CH-2012-1616
dc.identifier.eissn1875-8622
dc.identifier.embargoNO
dc.identifier.filenameinventorynoIR00120
dc.identifier.issn1386-0291
dc.identifier.quartileQ3
dc.identifier.scopus2-s2.0-84878835887
dc.identifier.urihttps://doi.org/10.3233/CH-2012-1616
dc.identifier.wos319733600010
dc.keywordsRed blood cell deformability
dc.keywordsHemorheology
dc.keywordsData reduction
dc.keywordsEktacytometry
dc.keywordsCardiovascular System
dc.keywordsCardiology
dc.language.isoeng
dc.publisherIOS Press
dc.relation.ispartofClinical Hemorheology and Microcirculation
dc.relation.urihttp://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/1151
dc.subjectMedicine
dc.subjectHematology
dc.titleData reduction methods for ektacytometry in clinical hemorheology
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorBaşkurt, Oğuz Kerim
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit2School of Medicine
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