Publication: Development of a mouse embryonic stem cell model for investigating the functions of the linker histone H1-4
Program
School / College / Institute
College of Sciences
GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
KU Authors
Co-Authors
Abu Alhaija, A.A.
Lone, I.N., Ozkuru Sekeroglu, E., Batur, T., Angelov, D.
Dimitrov, S.
Hamiche, A.
Yagci, T.
Alotaibi, H.
Diril, M.K.
Publication Date
Language
Type
Embargo Status
Journal Title
Journal ISSN
Volume Title
Alternative Title
Abstract
The linker histone H1 C-terminal domain (CTD) plays a pivotal role in chromatin condensation. De novo frameshift mutations within the CTD coding region of H1.4 have recently been reported to be associated with Rahman syndrome, a neurological disease that causes intellectual disability and overgrowth. To investigate the mechanisms and pathogenesis of Rahman syndrome, we developed a cellular model using murine embryonic stem cells (mESCs) and CRISPR/Cas9 genome engineering. Our engineered mES cells facilitate detailed investigations, such as H1-4 dynamics, immunoprecipitation, and nuclear localization; in addition, we tagged the mutant H1-4 with a photoactivatable GFP (PA-GFP) and an HA tag to facilitate pulldown assays. We anticipate that these engineered cells could also be used for the development of a mouse model to study the in vivo role of the H1-4 protein.
Source
Publisher
Wiley
Subject
Biochemistry and molecular biology
Citation
Has Part
Source
FEBS Open Bio
Book Series Title
Edition
DOI
10.1002/2211-5463.13750