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Acinar cell induced autolysis is a frequent occurrence in Cytolyt-fixed pancreatic fine needle aspiration specimens: an analysis of 157 cytology samples

dc.contributor.coauthorAlwelaie, Yazeed
dc.contributor.coauthorPoint du Jour, Kimberly S.
dc.contributor.coauthorPandya, Sonal
dc.contributor.coauthorGoodman, Abigail L.
dc.contributor.coauthorCenteno, Barbara A.
dc.contributor.coauthorReid, Michelle D.
dc.contributor.departmentKUH (Koç University Hospital)
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorAdsay, Nazmi Volkan
dc.contributor.schoolcollegeinstituteKUH (KOÇ UNIVERSITY HOSPITAL)
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T23:05:29Z
dc.date.issued2021
dc.description.abstractBackground Although 10% formalin is a standard preservative in pancreatic FNAs, the effect of CytoLyt on pancreatic tissue preservation has not been systematically explored. Methods Smears and cell blocks from CytoLyt-fixed (CF-CBs) and formalin-fixed (FF-CBs) pancreatic FNAs were blindly reviewed without knowledge of the fixative used, and the presence of tissue/tumor autolysis was noted. Controls included FF-CBs from pancreatic FNAs, CF-CBs from nonpancreatic FNAs, and 4 pancreatic FNAs with matched CF-CBs and FF-CBs. Results We found that 62 of 85 (73%) pancreatic FNAs with CF-CBs showed significant autolysis, which was most pronounced in acinar cells and/or tumor cells with benign acinar cells in the background, compared with 2 of 46 (4%) FF-CBs (P < .0001) and 3 of 26 (12%) CF-CBs from nonpancreatic FNAs (73% vs 12%; P < .0001). Of the 4 pancreatic FNAs with matched CF-CBs and FF-CBs, all 4 CF-CBs showed marked autolysis versus none of the matched FF-CBs. Of the 23 (27%) pancreatic FNAs with CF-CBs that did not show autolysis, 10 had no acinar cells, and 7 had only minute tissue fragments on CB. Conclusion While CytoLyt is a useful fixative for nonpancreatic FNAs it is a suboptimal fixative for pancreatic FNAs and is associated with tissue/tumor autolysis in the majority of cases, influencing morphologic evaluation, and potentially immunocytochemical staining. Autolysis appears to be due to acinar enzymes whose effect is likely interrupted/inhibited by formalin fixation. Cytopathologists and cytotechnologists should be mindful of this pitfall and should avoid using CytoLyt as a fixative for pancreatic FNAs.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue4
dc.description.openaccessNO
dc.description.sponsoredbyTubitakEuN/A
dc.description.volume129
dc.identifier.doi10.1002/cncy.22378
dc.identifier.eissn1934-6638
dc.identifier.issn1934-662X
dc.identifier.scopus2-s2.0-85096762499
dc.identifier.urihttps://doi.org/10.1002/cncy.22378
dc.identifier.urihttps://hdl.handle.net/20.500.14288/8816
dc.identifier.wos583446700001
dc.keywordsCytoLyt
dc.keywordsCytology
dc.keywordsFixation
dc.keywordsFixative
dc.keywordsFNA
dc.keywordsPancreas
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofCancer Cytopathology
dc.subjectOncology
dc.subjectPathology
dc.titleAcinar cell induced autolysis is a frequent occurrence in Cytolyt-fixed pancreatic fine needle aspiration specimens: an analysis of 157 cytology samples
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorAdsay, Nazmi Volkan
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1KUH (KOÇ UNIVERSITY HOSPITAL)
local.publication.orgunit2KUH (Koç University Hospital)
local.publication.orgunit2School of Medicine
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