Publication: Acinar cell induced autolysis is a frequent occurrence in Cytolyt-fixed pancreatic fine needle aspiration specimens: an analysis of 157 cytology samples
dc.contributor.coauthor | Alwelaie, Yazeed | |
dc.contributor.coauthor | Point du Jour, Kimberly S. | |
dc.contributor.coauthor | Pandya, Sonal | |
dc.contributor.coauthor | Goodman, Abigail L. | |
dc.contributor.coauthor | Centeno, Barbara A. | |
dc.contributor.coauthor | Reid, Michelle D. | |
dc.contributor.department | KUH (Koç University Hospital) | |
dc.contributor.department | School of Medicine | |
dc.contributor.kuauthor | Adsay, Nazmi Volkan | |
dc.contributor.schoolcollegeinstitute | KUH (KOÇ UNIVERSITY HOSPITAL) | |
dc.contributor.schoolcollegeinstitute | SCHOOL OF MEDICINE | |
dc.date.accessioned | 2024-11-09T23:05:29Z | |
dc.date.issued | 2021 | |
dc.description.abstract | Background Although 10% formalin is a standard preservative in pancreatic FNAs, the effect of CytoLyt on pancreatic tissue preservation has not been systematically explored. Methods Smears and cell blocks from CytoLyt-fixed (CF-CBs) and formalin-fixed (FF-CBs) pancreatic FNAs were blindly reviewed without knowledge of the fixative used, and the presence of tissue/tumor autolysis was noted. Controls included FF-CBs from pancreatic FNAs, CF-CBs from nonpancreatic FNAs, and 4 pancreatic FNAs with matched CF-CBs and FF-CBs. Results We found that 62 of 85 (73%) pancreatic FNAs with CF-CBs showed significant autolysis, which was most pronounced in acinar cells and/or tumor cells with benign acinar cells in the background, compared with 2 of 46 (4%) FF-CBs (P < .0001) and 3 of 26 (12%) CF-CBs from nonpancreatic FNAs (73% vs 12%; P < .0001). Of the 4 pancreatic FNAs with matched CF-CBs and FF-CBs, all 4 CF-CBs showed marked autolysis versus none of the matched FF-CBs. Of the 23 (27%) pancreatic FNAs with CF-CBs that did not show autolysis, 10 had no acinar cells, and 7 had only minute tissue fragments on CB. Conclusion While CytoLyt is a useful fixative for nonpancreatic FNAs it is a suboptimal fixative for pancreatic FNAs and is associated with tissue/tumor autolysis in the majority of cases, influencing morphologic evaluation, and potentially immunocytochemical staining. Autolysis appears to be due to acinar enzymes whose effect is likely interrupted/inhibited by formalin fixation. Cytopathologists and cytotechnologists should be mindful of this pitfall and should avoid using CytoLyt as a fixative for pancreatic FNAs. | |
dc.description.indexedby | WOS | |
dc.description.indexedby | Scopus | |
dc.description.indexedby | PubMed | |
dc.description.issue | 4 | |
dc.description.openaccess | NO | |
dc.description.sponsoredbyTubitakEu | N/A | |
dc.description.volume | 129 | |
dc.identifier.doi | 10.1002/cncy.22378 | |
dc.identifier.eissn | 1934-6638 | |
dc.identifier.issn | 1934-662X | |
dc.identifier.scopus | 2-s2.0-85096762499 | |
dc.identifier.uri | https://doi.org/10.1002/cncy.22378 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14288/8816 | |
dc.identifier.wos | 583446700001 | |
dc.keywords | CytoLyt | |
dc.keywords | Cytology | |
dc.keywords | Fixation | |
dc.keywords | Fixative | |
dc.keywords | FNA | |
dc.keywords | Pancreas | |
dc.language.iso | eng | |
dc.publisher | Wiley | |
dc.relation.ispartof | Cancer Cytopathology | |
dc.subject | Oncology | |
dc.subject | Pathology | |
dc.title | Acinar cell induced autolysis is a frequent occurrence in Cytolyt-fixed pancreatic fine needle aspiration specimens: an analysis of 157 cytology samples | |
dc.type | Journal Article | |
dspace.entity.type | Publication | |
local.contributor.kuauthor | Adsay, Nazmi Volkan | |
local.publication.orgunit1 | SCHOOL OF MEDICINE | |
local.publication.orgunit1 | KUH (KOÇ UNIVERSITY HOSPITAL) | |
local.publication.orgunit2 | KUH (Koç University Hospital) | |
local.publication.orgunit2 | School of Medicine | |
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