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Comparison of astrocytes and gap junction proteins in the white matter of genetic absence epileptic and control rats: an experimental study

dc.contributor.coauthorÖzkan, Mazhar
dc.contributor.departmentSchool of Medicine
dc.contributor.departmentGraduate School of Health Sciences
dc.contributor.kuauthorÇavdar, Safiye
dc.contributor.kuauthorSur, İlknur Erdem
dc.contributor.kuauthorKöse, Büşra
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF HEALTH SCIENCES
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T23:37:50Z
dc.date.issued2022
dc.description.abstractObjectives The role of white matter astrocytes in absence epilepsy is unknown. The present study aims to quantify astrocytic markers glial fibrillary acidic protein (GFAP), gap junction's proteins connexin 30 (Cx30) and connexin 43 (Cx43) in the corpus callosum (CC) of genetic absence epileptic rats from Strasbourg (GAERS), Wistar albino glaxo rats from Rijswijk (WAG/Rij)and compare the results with control animals. Methods -The density of GFAP, Cx30 and Cx43 positive astrocytes in per unite area were quantified in the CC of GAERS, WAG/Rij and control animals using immunohistochemistry and real-time quantitative polymerase chain reaction (RT-qPCR). The quantifications were made from three regions of CC; below the primary somatosensory (S1BF), below the motor (M1) and below the retrosplenial (RSG) cortices. Results oThe number GFAP, Cx30 and Cx43 immunopositive astrocytes showed heterogeneous distribution within the CC. The GFAP immunopositive astrocytes was significantly high in the S1BF region of the three strains. The immunopositive GFAP and Cx43 showed significant decrease in the S1BF and M1 regions in GAERS and WAG/Rij compared to control animals, however, an increase in the immunopositive Cx30 was observed in the same regions in both GAERS and WAG/Rij compared to control Wistar animals but the increase was significant for GAERS but not for WAG/Rij. The RT-qPCR analysis was corroborated by GFAP immunohistochemistry results. Conclusion The different expression pattern of the two Cx's in the CC of the epileptic strains compared to control animals may indicate a compensatory response or maybe the cause of generalization of absence seizures.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue8
dc.description.openaccessNO
dc.description.sponsoredbyTubitakEuN/A
dc.description.volume44
dc.identifier.doi10.1080/01616412.2022.2039527
dc.identifier.eissn1743-1328
dc.identifier.issn0161-6412
dc.identifier.scopus2-s2.0-85125254252
dc.identifier.urihttps://doi.org/10.1080/01616412.2022.2039527
dc.identifier.urihttps://hdl.handle.net/20.500.14288/12878
dc.identifier.wos754947900001
dc.keywordsCorpus callosum
dc.keywordsconnexin
dc.keywordsglial fibrillary acidic protein
dc.keywordsabsence epilepsy
dc.keywordsimmunohistochemistry
dc.keywordsrt-qPCR
dc.keywordsCorpus-callosum
dc.keywordsSpontaneous spike
dc.keywordsWave discharges
dc.keywordsGlial-cells
dc.keywordsExpression
dc.keywordsSeizures
dc.keywordsNetworks
dc.keywordsOligodendrocytes
dc.keywordsNeocortex
dc.keywordsChannels
dc.language.isoeng
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofNeurological Research
dc.subjectClinical neurology
dc.subjectNeurosciences
dc.titleComparison of astrocytes and gap junction proteins in the white matter of genetic absence epileptic and control rats: an experimental study
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorÇavdar, Safiye
local.contributor.kuauthorKöse, Büşra
local.contributor.kuauthorSur, İlknur Erdem
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1GRADUATE SCHOOL OF HEALTH SCIENCES
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Health Sciences
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