Publication:
Estimating tumor proportion in smear slides for reliable molecular analysis

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dc.contributor.departmentSchool of Medicine
dc.contributor.departmentKUIS AI (Koç University & İş Bank Artificial Intelligence Center)
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.kuauthorFırat, Pınar Arıkan
dc.contributor.kuauthorKulaç, İbrahim
dc.contributor.kuauthorMeriçöz, Çisel Aydın
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.contributor.schoolcollegeinstituteResearch Center
dc.date.accessioned2025-12-31T08:20:58Z
dc.date.available2025-12-31
dc.date.issued2025
dc.description.fulltextYes
dc.description.harvestedfromManual
dc.description.indexedbyPubMed
dc.description.publisherscopeNational
dc.description.readpublishN/A
dc.description.sponsoredbyTubitakEuN/A
dc.identifier.doi10.5146/tjpath.2025.13923
dc.identifier.embargoNo
dc.identifier.pubmed41058556
dc.identifier.quartileN/A
dc.identifier.urihttps://doi.org/10.5146/tjpath.2025.13923
dc.identifier.urihttps://hdl.handle.net/20.500.14288/31559
dc.keywordsTumor proportion
dc.keywordsCytology smears
dc.keywordsMolecular analysis
dc.language.isoeng
dc.relation.affiliationKoç University
dc.relation.collectionKoç University Institutional Repository
dc.relation.ispartofTurkish Journal of Pathology
dc.relation.openaccessYes
dc.rightsCC BY-NC-ND (Attribution-NonCommercial-NoDerivs)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectPathology
dc.subjectPathology, Objective: The use of molecular pathology is critical in diagnostics and theranostics. Today, cytological smears are utilized for molecular testing more often than ever. Accurate tumor cell percentage estimation is essential for reliable molecular testing, but its consistency remains uncertain. This study evaluates the reliability of tumor cell percentage estimations among an expert cytopathologist, a molecular cytopathologist, and a molecular pathologist. Material and Methods: Digital images from May-Grünwald-Giemsa (MGG)-stained smear slides of ten EBUS-guided mediastinal lymph node samples were selected. Five regions per slide were evaluated (50 areas from 10 patients). Three pathologists independently estimated tumor cell percentages using predefined categories (0–10%, 11–20%, 21–50%, etc.). Cells were also counted manually as the gold standard. Results: The molecular cytopathologist (Observer 1 -) showed the highest consistency (Kappa = 0.69), followed by the expert cytopathologist (Observer 3 -, Kappa = 0.64), both demonstrating substantial agreement with the gold standard. The molecular pathologist (Observer 2 -) displayed moderate consistency (Kappa = 0.52). Agreement was most significant in the 71–100% category, aligning in over 95% of cases. The lowest value occurred in the 11–20% category. In this category, tumor proportions were frequently overestimated compared to the gold standard. Conclusion: Variability in tumor percentage estimations shows the need for standardized protocols and training. Substantial agreement was reached in specific categories. However, discrepancies in borderline cases highlight the importance of accurate assessments. More research is needed to improve estimation methods.
dc.titleEstimating tumor proportion in smear slides for reliable molecular analysis
dc.typeJournal Article
dspace.entity.typePublication
person.familyNameFırat
person.familyNameKulaç
person.familyNameMeriçöz
person.givenNamePınar Arıkan
person.givenNameİbrahim
person.givenNameÇisel Aydın
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