Publication: Control of heparin surface density in multilayers of partially hydrolyzed poly(2-ethyl-2-oxazoline) by degree of hydrolysis
dc.contributor.department | Department of Chemistry | |
dc.contributor.department | KUYTAM (Koç University Surface Science and Technology Center) | |
dc.contributor.department | Graduate School of Sciences and Engineering | |
dc.contributor.kuauthor | Saadatlou, Ghazaleh Azizi | |
dc.contributor.kuauthor | Güner, Pınar Tatar | |
dc.contributor.kuauthor | Demirel, Adem Levent | |
dc.contributor.schoolcollegeinstitute | GRADUATE SCHOOL OF SCIENCES AND ENGINEERING | |
dc.contributor.schoolcollegeinstitute | College of Sciences | |
dc.contributor.schoolcollegeinstitute | Research Center | |
dc.date.accessioned | 2024-12-29T09:38:37Z | |
dc.date.issued | 2024 | |
dc.description.abstract | Controlling the surface density of heparin in active coatings is important in providing anticoagulation while preventing bleeding. An approach based on tuning the degree of hydrolysis of poly(2-ethyl-2-oxazoline) (PEOX) is presented to control the surface density of heparin in layer-by-layer (LbL) assembled films. Multilayers are prepared at pH 5 in 0.5 M aqueous NaCl solutions by electrostatic interactions between negatively charged heparin and the positively charged amine groups in hydrolyzed PEOX. Characterization of the multilayers by quartz crystal microbalance with dissipation (QCM-D), toluidine blue (TBO) assay and X-ray Photoelectron Spectroscopy (XPS) all shows that the amount of heparin deposited increases with the degree of hydrolysis. While non-hydrolyzed PEOX/heparin multilayers do not grow, the average deposited mass per area per bilayer as determined by QCM-D measurements increases with the degree of hydrolysis. At 50% hydrolysis, TBO assay gives a heparin surface density of 1.03 mu g/cm(2) and atomic % of sulfur as determined by XPS leveled off at similar to 14%. These results show the potential of acidic hydrolysis of PEOX combined with LbL assembly of heparin as a reproducible method for controlling the surface density of heparin in anticoagulant coatings. | |
dc.description.indexedby | WoS | |
dc.description.indexedby | Scopus | |
dc.description.issue | 18 | |
dc.description.publisherscope | International | |
dc.description.volume | 62 | |
dc.identifier.doi | 10.1002/pol.20240185 | |
dc.identifier.eissn | 2642-4169 | |
dc.identifier.issn | 2642-4150 | |
dc.identifier.quartile | Q2 | |
dc.identifier.scopus | 2-s2.0-85196298579 | |
dc.identifier.uri | https://doi.org/10.1002/pol.20240185 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14288/22751 | |
dc.identifier.wos | 1251443700001 | |
dc.keywords | Heparin surface density | |
dc.keywords | Multilayers | |
dc.keywords | Poly(2-ethyl-2-oxazoline)-co-polyethyleneimine | |
dc.language | en | |
dc.publisher | Wiley | |
dc.source | Journal of Polymer Science | |
dc.subject | Polymer science | |
dc.title | Control of heparin surface density in multilayers of partially hydrolyzed poly(2-ethyl-2-oxazoline) by degree of hydrolysis | |
dc.type | Journal article | |
dspace.entity.type | Publication | |
local.contributor.kuauthor | Saadatlou, Ghazaleh Azizi | |
local.contributor.kuauthor | Güner, Pınar Tatar | |
local.contributor.kuauthor | Demirel, Adem Levent | |
local.publication.orgunit1 | GRADUATE SCHOOL OF SCIENCES AND ENGINEERING | |
local.publication.orgunit1 | College of Sciences | |
local.publication.orgunit1 | Research Center | |
local.publication.orgunit2 | Department of Chemistry | |
local.publication.orgunit2 | KUYTAM (Koç University Surface Science and Technology Center) | |
local.publication.orgunit2 | Graduate School of Sciences and Engineering | |
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