Publication:
Control of heparin surface density in multilayers of partially hydrolyzed poly(2-ethyl-2-oxazoline) by degree of hydrolysis

dc.contributor.departmentDepartment of Chemistry
dc.contributor.departmentKUYTAM (Koç University Surface Science and Technology Center)
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.kuauthorSaadatlou, Ghazaleh Azizi
dc.contributor.kuauthorGüner, Pınar Tatar
dc.contributor.kuauthorDemirel, Adem Levent
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.contributor.schoolcollegeinstituteResearch Center
dc.date.accessioned2024-12-29T09:38:37Z
dc.date.issued2024
dc.description.abstractControlling the surface density of heparin in active coatings is important in providing anticoagulation while preventing bleeding. An approach based on tuning the degree of hydrolysis of poly(2-ethyl-2-oxazoline) (PEOX) is presented to control the surface density of heparin in layer-by-layer (LbL) assembled films. Multilayers are prepared at pH 5 in 0.5 M aqueous NaCl solutions by electrostatic interactions between negatively charged heparin and the positively charged amine groups in hydrolyzed PEOX. Characterization of the multilayers by quartz crystal microbalance with dissipation (QCM-D), toluidine blue (TBO) assay and X-ray Photoelectron Spectroscopy (XPS) all shows that the amount of heparin deposited increases with the degree of hydrolysis. While non-hydrolyzed PEOX/heparin multilayers do not grow, the average deposited mass per area per bilayer as determined by QCM-D measurements increases with the degree of hydrolysis. At 50% hydrolysis, TBO assay gives a heparin surface density of 1.03 mu g/cm(2) and atomic % of sulfur as determined by XPS leveled off at similar to 14%. These results show the potential of acidic hydrolysis of PEOX combined with LbL assembly of heparin as a reproducible method for controlling the surface density of heparin in anticoagulant coatings.
dc.description.indexedbyWoS
dc.description.indexedbyScopus
dc.description.issue18
dc.description.publisherscopeInternational
dc.description.volume62
dc.identifier.doi10.1002/pol.20240185
dc.identifier.eissn2642-4169
dc.identifier.issn2642-4150
dc.identifier.quartileQ2
dc.identifier.scopus2-s2.0-85196298579
dc.identifier.urihttps://doi.org/10.1002/pol.20240185
dc.identifier.urihttps://hdl.handle.net/20.500.14288/22751
dc.identifier.wos1251443700001
dc.keywordsHeparin surface density
dc.keywordsMultilayers
dc.keywordsPoly(2-ethyl-2-oxazoline)-co-polyethyleneimine
dc.languageen
dc.publisherWiley
dc.sourceJournal of Polymer Science
dc.subjectPolymer science
dc.titleControl of heparin surface density in multilayers of partially hydrolyzed poly(2-ethyl-2-oxazoline) by degree of hydrolysis
dc.typeJournal article
dspace.entity.typePublication
local.contributor.kuauthorSaadatlou, Ghazaleh Azizi
local.contributor.kuauthorGüner, Pınar Tatar
local.contributor.kuauthorDemirel, Adem Levent
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1College of Sciences
local.publication.orgunit1Research Center
local.publication.orgunit2Department of Chemistry
local.publication.orgunit2KUYTAM (Koç University Surface Science and Technology Center)
local.publication.orgunit2Graduate School of Sciences and Engineering
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