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Selective detection of carboxylesterase 2 activity in cancer cells using an activity-based chemiluminescent probe

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dc.contributor.coauthorKailass, Karishma
dc.contributor.coauthorDigby, Elyse M.
dc.contributor.coauthorBeharry, Andrew A.
dc.contributor.departmentDepartment of Chemistry
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.departmentKUBAM (Koç University Boron and Advanced Materials Application and Research Center)
dc.contributor.departmentKUYTAM (Koç University Surface Science and Technology Center)
dc.contributor.kuauthorAlmammadov, Toghrul
dc.contributor.kuauthorKölemen, Safacan
dc.contributor.kuauthorÖzsan, Çağrı
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteResearch Center
dc.date.accessioned2024-11-09T22:56:32Z
dc.date.issued2022
dc.description.abstractCarboxylesterase 2 (CES2) has crucial roles in both xenobiotic metabolism and formation of pathogenic states including cancer. Thus, it is highly critical to monitor intracellular CES2 activity in living cancer cells. Here, we report a CES2 activatable phenoxy 1,2-dioxetane based chemiluminescent agent (CL-CES2). The probe exhibited a selective turn-on response in the presence of CES2 enzyme and enabled detection of CES2 activity in three different cancer cells that possess varying enzyme concentrations with high signal to noise ratios. In contrast no signal was obtained with CES1, an isoform of CES2 enzyme. CL-CES2 marks the first ever example of a CES2-responsive chemiluminescent luminophore and holds a great potential in further understanding the roles of CES2 activity in tumorogenesis.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue78
dc.description.openaccessNO
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipCancer Research Society [25210] A. A. B. thanks Grant 25210 from the Cancer Research Society for the funding.
dc.description.volume58
dc.identifier.doi10.1039/d2cc03309g
dc.identifier.eissn1364-548X
dc.identifier.issn1359-7345
dc.identifier.scopus2-s2.0-85138654434
dc.identifier.urihttps://doi.org/10.1039/d2cc03309g
dc.identifier.urihttps://hdl.handle.net/20.500.14288/7385
dc.identifier.wos850236700001
dc.keywordsFluorescent-probe
dc.keywordsDesign
dc.keywordsTissue
dc.language.isoeng
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofChemical Communications
dc.subjectChemistry
dc.titleSelective detection of carboxylesterase 2 activity in cancer cells using an activity-based chemiluminescent probe
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorÖzsan, Çağrı
local.contributor.kuauthorAlmammadov, Toghrul
local.contributor.kuauthorKölemen, Safacan
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1College of Sciences
local.publication.orgunit1Research Center
local.publication.orgunit2Department of Chemistry
local.publication.orgunit2KUYTAM (Koç University Surface Science and Technology Center)
local.publication.orgunit2KUBAM (Koç University Boron and Advanced Materials Application and Research Center)
local.publication.orgunit2Graduate School of Sciences and Engineering
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