Publication: Melatonin improves reduced activities of membrane atpases and preserves ultrastructure of gray and white matter in the rat brain ischemia/reperfusion model
Program
KU-Authors
KU Authors
Co-Authors
Tuncer, Meltem
Pehlivanoğlu, Bilge
İsbir, Turgay
Advisor
Publication Date
2021
Language
English
Type
Journal Article
Journal Title
Journal ISSN
Volume Title
Abstract
Ischemia/reperfusion (I/R) is among the most frequent neurological problems and early intervention to limit the damage is crucial in decreasing mortality and morbidity. Based on reports regarding beneficial effects of melatonin, we investigated its impact on Na+-K+/Mg2+ ATPase and Ca2+/Mg2+ ATPase activities and ultrastructure of gray and white matter in the rat forebrain I/R model. Adult Wistar-albino rats (n = 78), were randomized into control, ischemia (I), ischemia/reperfusion (I/R), low (I/R + melatonin 400 mu g/kg), moderate (I/R + melatonin 1200 mu g/kg), and high (I/R + melatonin 2400 mu g/kg) dose melatonin. Two-vessel occlusion combined with hypotension (15 min) induced ischemia and reperfusion (75 min) achieved by blood reinfusion were performed. Activities of the membrane-bound enzyme, brain malondialdehyde levels, and brain matter ultrastructure were examined in frontoparietal cortices. Melatonin lowered production of malondialdehyde in a dose-dependently. The enzyme activities attenuated under I and I/R, improved with melatonin treatment. I and I/R severely disturbed gray and white matter morphology. Melatonin, in all applied doses, decreased ultrastructural damages in both gray and white matter. Favorable effects of melatonin can be attributed to its antioxidant properties suggesting that it could be a promising neuroprotective agent against I/R injury being effective both for gray and white matter due to favorable biological properties.
Description
Source:
Biochemistry-Moscow
Publisher:
Springer
Keywords:
Subject
Biochemistry, Molecular biology