Publication:
Unfolded protein response is involved in trans-platinum (ii) complex-induced apoptosis in prostate cancer cells via ros accumulation

dc.contributor.coauthorKarakaş, Didem
dc.contributor.coauthorOral, Arzu Y.
dc.contributor.coauthorYılmaz, Veysel T.
dc.contributor.coauthorUlukaya, Engin
dc.contributor.departmentAnimal Laboratory
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.kuauthorCevatemre, Buse
dc.contributor.schoolcollegeinstituteLaboratory
dc.contributor.schoolcollegeinstituteResearch Center
dc.date.accessioned2024-11-10T00:12:18Z
dc.date.issued2019
dc.description.abstractBackground: Prostate cancer is one of the most common cancer types and it is the sixth leading cause of cancer-related death in men worldwide. Even though novel treatment modalities have been developed, it still a lifethreatening disease. Therefore novel compounds are needed to improve the overall survival. Methods: In our study, it was aimed to evaluate the anti-cancer activity of newly synthesized Platinum (II) [Pt(II)] complex on DU145, LNCaP and PC-3 prostate cancer cell lines. The cytotoxic activity of Pt(II) complex was tested by SRB and ATP cell viability assays. To detect the mode of cell death; fluorescent staining, flow cytometry and western blot analyses were performed. Results: The Pt(II) complex treatment resulted in a decrease in cell viability and increasing levels of apoptotic markers (pyknotic nuclei, annexin-V, caspase 3/7 activity) and a decrease in mitochondrial membrane potential in a dose dependent manner. Among cell types, tested PC-3 cells were found to be more sensitive to Pt(II) complex, demonstrating elevation of DNA damage in this cell line. In addition, Pt(II) complex induced Endoplasmic Reticulum (ER) stress by triggering ROS generation. More importantly, pre-treatment with NAC alleviated Pt(II) complex-mediated ER stress and cell death in PC-3. Conclusion: These findings suggest an upstream role of ROS production in Pt(II) complex-induced ER stressmediated apoptotic cell death. Considering the ROS-mediated apoptosis inducing the effect of Pt(II) complex, it warrants further evaluation as a novel metal-containing anticancer drug candidate.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue9
dc.description.openaccessNO
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.volume19
dc.identifier.doi10.2174/1871520619666190409103334
dc.identifier.eissn1875-5992
dc.identifier.issn1871-5206
dc.identifier.quartileQ4
dc.identifier.scopus2-s2.0-85074963173
dc.identifier.urihttps://doi.org/10.2174/1871520619666190409103334
dc.identifier.urihttps://hdl.handle.net/20.500.14288/17637
dc.identifier.wos492373400010
dc.language.isoeng
dc.relation.ispartofAnti-Cancer Agents in Medicinal Chemistry
dc.subjectOncology
dc.subjectChemistry
dc.subjectMedicinal
dc.titleUnfolded protein response is involved in trans-platinum (ii) complex-induced apoptosis in prostate cancer cells via ros accumulation
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorCevatemre, Buse
local.publication.orgunit1Laboratory
local.publication.orgunit1Research Center
local.publication.orgunit2Animal Laboratory
local.publication.orgunit2KUTTAM (Koç University Research Center for Translational Medicine)
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