Publication:
Day-time isoflurane administration suppresses circadian gene expressions in both the brain and a peripheral Organ, Liver

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SCHOOL OF MEDICINE
Upper Org Unit

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Gökmen, Necati
Barış, İbrahim
Öçmen, Elvan
Yılmaz, Osman
Günerli, Ali

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NO

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Gündüz uygulanan izofluran anestezisi beyinde ve periferik bir organ olan karaciğerde sirkadiyen saat genlerini baskılar

Abstract

Objective: The aim of this study is to investigate the effects of light and administration time of isoflurane on circadian gene expression in the brains and liver tissues of rats kept in light-dark cycle. Methods: Seventy tw o 15-days-old rats pups were divided into four groups. All animals were exposed to 1.5% concentration of isoflurane or to 6 L min(-1) O-2 for six hours between Zeitgeber Time (ZT) 0-ZT06 ( day-time administration) or ZT12-ZT18 ( night-time administration). Rats were sacrificed after six hours of anaesthesia with four-hour time intervals. Total RNA was isolated from brains and liver tissues. Circadian gene expression was examined using quantitative real-time Reverse transcription polymerase chain reaction (RT-PCR). Results: BMAL1, CLOCK, PER2 and CRY2 gene expression levels were markedly suppressed after day-time anaesthesia in the both brain and liver, but night-time administration caused only temporary suppression of gene expression. Conclusion: The effect of isoflurane on the circadian clock is time-dependent, and administered isoflurane anaesthesia at night had minimal effect on clock gene expression. Additionally, when the treated animals were kept in a regular light-dark cycle, isoflurane-induced phase shift was not observed, possibly because of the light.

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Turkish Society of Anaesthesiology and Reanimation

Subject

Medicine, Anesthesiology

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Has Part

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Turk Anesteziyoloji Ve Reanimasyon Dernegi Dergisi

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DOI

10.5152/TJAR.2017.68466

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