Publication:
Association between gene polymorphisms in TIM1, TSLP, IL18R1 and childhood asthma in Turkish population

dc.contributor.coauthorMete, Fatih
dc.contributor.coauthorÖzkaya, Emin
dc.contributor.coauthorAras, Şükrü
dc.contributor.coauthorKöksal, Vedat
dc.contributor.coauthorEtlik, Özdal
dc.contributor.departmentDepartment of Molecular Biology and Genetics
dc.contributor.kuauthorBarış, İbrahim
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.date.accessioned2024-11-09T13:45:33Z
dc.date.issued2014
dc.description.abstractMany immunologic and inflammatory mechanisms play a role in asthma etiology. The aim of this study was to investigate the susceptibility of asthma patients in the Turkish population with demonstrating genes for polymorphisms in TIM1, TSLP and IL18R1. All of the genomic DNA samples were isolated from blood samples according to a standard salting-out protocol. DNA samples were stored at -20 degrees C until the genotype analysis was performed. rs3806933 (TSLP -847 C > T) and TIM1 -416G > C were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The rs3806933 (TSLP -847 C > T) was genotyped by PCR using our new primers and HphI restriction enzyme digestion. rs2287033 (IL18R1 c. 1270+150 A > G), rs3213733 (IL18R1 c. 626-196 G > T), and rs3771166 (IL18R1-c. 302+1694 C > T) were genotyped using SYBR green dye based real time PCR assay. Results: The allele frequencies of 5 SNPs in TSLP, TIM-1, and IL18R1 genes were determined in 139 asthmatic patients and 126 healthy controls of in Turkish population. The investigated SNPs are as follows; rs3806933 (TSLP -847 C > T), TIM1 -416G > C, rs2287033 (IL18R1 c. 1270+150 A > G), rs3213733 (IL18R1 c. 626-196 G > T), and rs3771166 (IL18R1-c. 302+1694 C > T). Results suggest that IL18R1 c. 626-196 G > T (rs3213733) and TIM1 -416G > C are significantly associated with asthma in patients in Turkish population. Patients with AA genotypes of rs2287033 (IL18R1 c. 1270+150 A > G), have significantly less total serum IgE levels when compared with patients having GG or GA genotypes (p < 0.012; 381.77 +/- 239.46 vs 557.52 +/- 549.96, respectively). Conclusion: This study showed that IL18R1 c. 626 -196 G > T (rs3213733) and TIM1 -416G > C are significantly associated with asthma patients in Turkish population.
dc.description.fulltextYES
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue4
dc.description.openaccessYES
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipN/A
dc.description.versionPublisher version
dc.description.volume7
dc.identifier.doi10.1007/s00408-017-0006-5
dc.identifier.embargoNO
dc.identifier.filenameinventorynoIR00917
dc.identifier.issn1940-5901
dc.identifier.quartileN/A
dc.identifier.scopus2-s2.0-84900463137
dc.identifier.urihttps://hdl.handle.net/20.500.14288/3629
dc.identifier.wos336740600037
dc.keywordsAsthma
dc.keywordsGenetics
dc.keywordsSNP polymorphism
dc.keywordsResearch and experimental medicine
dc.language.isoeng
dc.publishere-Century Publishing Corporation
dc.relation.ispartofInternational Journal of Clinical and Experimental Medicine
dc.relation.urihttp://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/918
dc.subjectMedicine
dc.subjectMolecular biology and genetics
dc.titleAssociation between gene polymorphisms in TIM1, TSLP, IL18R1 and childhood asthma in Turkish population
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorBarış, İbrahim
local.publication.orgunit1College of Sciences
local.publication.orgunit2Department of Molecular Biology and Genetics
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relation.isOrgUnitOfPublication.latestForDiscoveryaee2d329-aabe-4b58-ba67-09dbf8575547
relation.isParentOrgUnitOfPublicationaf0395b0-7219-4165-a909-7016fa30932d
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