Publication:
Critical role of the composition of the cell culture medium on cell attachment and viability on PLA biocomposite scaffolds under in vitro assay conditions

dc.contributor.departmentDepartment of Chemistry
dc.contributor.departmentDepartment of Molecular Biology and Genetics
dc.contributor.departmentDepartment of Chemical and Biological Engineering
dc.contributor.kuauthorAtay, İpek
dc.contributor.kuauthorKavaklı, İbrahim Halil
dc.contributor.kuauthorSürme, Saliha
dc.contributor.kuauthorYılgör, Emel
dc.contributor.kuauthorYılgör, İskender
dc.contributor.schoolcollegeinstituteCollege of Sciences
dc.contributor.schoolcollegeinstituteCollege of Engineering
dc.date.accessioned2024-12-29T09:39:29Z
dc.date.issued2024
dc.description.abstractIn tissue engineering applications 3D scaffolds undertake the function of the native extracellular matrix (ECM) for cell attachment, survival, proliferation, and differentiation. For successful long-term implementation scaffolds need to mimic the ECM in vitro and in vivo. Many studies have focused on protein adsorption as the most critical factor for cell attachment. To improve protein adsorption on polymeric scaffolds various physical and chemical surface modification techniques, such as changing surface porosity, surface energy, and introducing various functional surface chemical groups have been investigated. It is well studied and documented that complex interactions between the biomaterial surface and the cell culture components in in vitro assay conditions define the scaffold performance. In this study influence of in vitro assay components on HepG2 liver cell attachment and proliferation on 3D printed poly(lactic acid) (PLA) scaffolds coated with various polymeric electrospun webs were investigated. MTT assays were performed on the scaffolds by using 3 different cell culture media;(i) basal cell culture medium (DMEM), (ii) DMEM supplemented with 10% albumin, and (iii) DMEM supplemented with 10% fetal bovine serum (FBS). Results obtained have shown dramatic improvement in cell attachment and proliferation in DMEM supplemented with 10% FBS when compared with DMEM and DMEM containing 10% albumin. Our findings clearly demonstrate synergistic effects of all bioactive components present in the FBS medium in significantly improving the HepG2 cell attachment and proliferation on polymeric scaffolds under in vitro conditions, regardless of the substrate structure, composition, and surface properties.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.volume297
dc.identifier.doi10.1016/j.polymer.2024.126823
dc.identifier.eissn1873-2291
dc.identifier.issn0032-3861
dc.identifier.quartileQ1
dc.identifier.scopus2-s2.0-85186582987
dc.identifier.urihttps://doi.org/10.1016/j.polymer.2024.126823
dc.identifier.urihttps://hdl.handle.net/20.500.14288/23007
dc.identifier.wos1208462400001
dc.keywords3D polymeric scaffolds
dc.keywordsProtein adsorption
dc.keywordsCell attachment
dc.language.isoeng
dc.publisherElsevier Sci Ltd
dc.relation.ispartofPolymer
dc.subjectPolymer science
dc.titleCritical role of the composition of the cell culture medium on cell attachment and viability on PLA biocomposite scaffolds under in vitro assay conditions
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorAtay, İpek
local.contributor.kuauthorYılgör, Emel
local.contributor.kuauthorSürme, Saliha
local.contributor.kuauthorKavaklı, İbrahim Halil
local.contributor.kuauthorYılgör, İskender
local.publication.orgunit1College of Sciences
local.publication.orgunit2Department of Chemistry;Department of Molecular Biology and Genetics;Department of Chemical and Biological Engineering
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