Publication:
Aspergillus Carneus metabolite Averufanin induced cell cycle arrest and apoptotic cell death on cancer cell lines via inducing DNA damage

dc.contributor.coauthorOzkaya, Ferhat Can
dc.contributor.coauthorEbrahim, Weaam
dc.contributor.departmentKUTTAM (Koç University Research Center for Translational Medicine)
dc.contributor.departmentGraduate School of Health Sciences
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorSokullu, Emel
dc.contributor.kuauthorŞahin, İrem Durmaz
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF HEALTH SCIENCES
dc.contributor.schoolcollegeinstituteResearch Center
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2025-01-19T10:30:13Z
dc.date.issued2023
dc.description.abstractCancer is one of the leading causes of death worldwide, accounting for nearly 10 million deaths in 2020. Current treatment methods include hormone therapy, & gamma;-radiation, immunotherapy, and chemotherapy. Although chemotherapy is the most effective treatment, there are major obstacles posed by resistance mechanisms of cancer cells and side-effects of the drugs, thus the search for novel anti-cancer compounds, especially from natural sources, is crucial for cancer pharmaceutics research. One natural source worthy of investigation is fungal species. In this study, the cytotoxicity of 5 metabolic compounds isolated from filamentous fungus Aspergillus Carneus. Arugosin C, Averufin, Averufanin, Nidurifin and Versicolorin C were analyzed using NCI-SRB assay on 10 different cell lines of breast cancer, ovarian cancer, glioblastoma and non-tumorigenic cell lines. Averufanin showed highest cytotoxicity with lowest IC50 concentrations especially on breast cancer cells. Therefore, Averufanin was further investigated to enlighten cell death and molecular mechanisms of action involved. Cell cycle analysis showed increase in SubG1 phase suggesting apoptosis induction which was further confirmed by Annexin V and Caspase 3/7 Assays. H2A.X staining revealed accumulation of DNA damage in cells treated with Averufanin and finally western blot analysis validated DNA damage response and downstream effects of Averufanin treatment in various signaling pathways. Consequently, this study shows that Averufanin compound induces cell cycle arrest and cell death via apoptosis through causing DNA damage and can be contemplated and further explored as a new therapeutic strategy in breast cancer.
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.issue1
dc.description.openaccessGreen Published, gold
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipThis work was supported by Koc University School of Medicine. The authors gratefully acknowledge the use of the services and facilities of the Koc University Research Center for Translational & nbsp;Medicine (KUTTAM), funded by the Presidency of Turkey, Presidency of Strategy and Budget. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Ministry of Development.
dc.description.volume13
dc.identifier.doi10.1038/s41598-023-30775-w
dc.identifier.issn2045-2322
dc.identifier.quartileQ1
dc.identifier.scopus2-s2.0-85153446312
dc.identifier.urihttps://doi.org/10.1038/s41598-023-30775-w
dc.identifier.urihttps://hdl.handle.net/20.500.14288/26011
dc.identifier.wos1025233300001
dc.keywordsApoptosis
dc.keywordsAspergillus carneus
dc.keywordsCell cycle
dc.keywordsCell cycle checkpoints
dc.language.isoeng
dc.publisherNature Portfolio
dc.relation.grantnoKoc University School of Medicine; Presidency of Turkey, Presidency of Strategy and Budget
dc.relation.ispartofScientific Reports
dc.subjectMultidisciplinary sciences
dc.titleAspergillus Carneus metabolite Averufanin induced cell cycle arrest and apoptotic cell death on cancer cell lines via inducing DNA damage
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorSokullu, Emel
local.contributor.kuauthorDemirel, Deren
local.contributor.kuauthorŞahin, İrem Durmaz
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit1GRADUATE SCHOOL OF HEALTH SCIENCES
local.publication.orgunit1Research Center
local.publication.orgunit2KUTTAM (Koç University Research Center for Translational Medicine)
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Health Sciences
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