Publication:
Ocular surface changes in mice with streptozotocin-induced diabetes and diabetic polyneuropathy

dc.contributor.coauthorSchicht M, Farger J, Wedel S, Sisignano M, Scholich K, Geisslinger G, Perumal N, Grus FH, Singh S, Paulsen F, Lütjen-Drecoll E.
dc.contributor.departmentSchool of Medicine
dc.contributor.kuauthorŞahin, Afsun
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2025-01-19T10:34:03Z
dc.date.issued2023
dc.description.abstractPurpose: Diabetes mellitus (DM) is a leading risk factor for corneal neuropathy and dry eye disease (DED). Another common consequence of DM is diabetic peripheral polyneuropathy (DPN). Both complications affect around 50 % of the DM patients but the relationship between DM, DED and DPN remains unclear. Methods: In this study, we examined mice with early onset of DM and PN after streptozotocin (STZ)-induced diabetes (DPN). We compared the early morphological changes of the sciatic nerve, dorsal root and trigeminal ganglia with the changes in the ocular surface, including tear proteomic and we also investigated respective changes in the gene expressions and morphological alterations in the eye tissues involved in tear production. Results: The lacrimal gland, conjunctival goblet cells and cornea showed morphological changes along with alterations in tear proteins without any obvious signs of ocular surface inflammation. The gene expression for respectively altered tear proteins i.e., of Clusterin in cornea, Car6, Adh3a1, and Eef1a1 in eyelids, and Pigr in the lacrimal gland also showed significant changes compared to control mice. In the trigeminal ganglia like in the dorsal root ganglia neuronal cells showed swollen mitochondria and, in the latter, there was a significant increase of NADPH oxidases and MMP9 suggestive of oxidative and neuronal stress. In the dorsal root ganglia and the sciatic nerve, there was an upregulation of a number of pro-inflammatory cytokines and pain-mediating chemokines. Conclusion: The early ocular changes in DM Mice only affect the lacrimal gland. Which, is reflected in the tear film composition of DPN mice. Due to the high protein concentration in tear fluid in humans, proteomic analysis in addition to noninvasive investigation of goblet cells and cornea can serve as a tools for the early diagnosis of DPN, DED in clinical practice. Early treatment could delay or even prevent the ocular complications of DM such as DED and PN. © 2023
dc.description.indexedbyWOS
dc.description.indexedbyScopus
dc.description.indexedbyPubMed
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.volume31
dc.identifier.doi10.1016/j.jtos.2023.12.006
dc.identifier.eissn1937-5913
dc.identifier.quartileQ1
dc.identifier.scopus2-s2.0-85182222472
dc.identifier.urihttps://doi.org/10.1016/j.jtos.2023.12.006
dc.identifier.urihttps://hdl.handle.net/20.500.14288/26716
dc.identifier.wos1153885800001
dc.keywordsDiabetes mellitus (DM)
dc.keywordsDry eye disease (DED)
dc.keywordsOcular surface
dc.keywordsPolyneuropathies (PN)
dc.keywordsTear film
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofOcul Surf
dc.subjectMedicine
dc.titleOcular surface changes in mice with streptozotocin-induced diabetes and diabetic polyneuropathy
dc.typeJournal Article
dspace.entity.typePublication
local.contributor.kuauthorŞahin, Afsun
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit2School of Medicine
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