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Publication Metadata only A leucine aminopeptidase activatable photosensitizer for cancer cell selective photodynamic therapy action(Elsevier Sci Ltd, 2021) N/A; N/A; N/A; Department of Chemistry; N/A; Department of Physics; Department of Chemistry; Department of Chemistry; Arslan, Büşra; Bilici, Kübra; Demirci, Gözde; Almammadov, Toghrul; Khan, Minahil; Sennaroğlu, Alphan; Acar, Havva Funda Yağcı; Kölemen, Safacan; Master Student; PhD Student; Master Student; Researcher; PhD Student; Faculty Member; Faculty Member; Faculty Member; Department of Physics; Department of Chemistry; Koç University Surface Science and Technology Center (KUYTAM) / Koç Üniversitesi Yüzey Teknolojileri Araştırmaları Merkezi (KUYTAM); Koç University Boron and Advanced Materials Application and Research Center (KUBAM) / Koç Üniversitesi Bor ve İleri Malzemeler Uygulama ve Araştırma Merkezi (KUBAM); Koç University Tüpraş Energy Center (KUTEM) / Koç Üniversitesi Tüpraş Enerji Merkezi (KÜTEM); Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; College of Sciences; Graduate School of Sciences and Engineering; College of Sciences; College of Sciences; College of Sciences; N/A; N/A; N/A; N/A; N/A; 23851; 178902; 272051Activity based photosensitizers (PS) continue to attract great attention as they enable selective photodynamic therapy action on cancer cells while sparing normal cells even under light irradiation. Sensitivity to specific enzymes that are differentially overexpressed in cancer cells is crucial in the design of activatable PSs. In this direction, we report here, for the first time, a leucine aminopeptidase (LAP) activatable PDT agent (HCL), which is a red-shifted, water soluble and photostable brominated hemicyanine derivative. HCL was activated by endogenous LAP enzyme selectively in A549 (lung) and HCT116 (colon) cancer cells containing high LAP levels and induced effective photocytotoxicity with negligible dark toxicity. Furthermore, the fluorescence of the parent bromo-hemicyanine core was restored upon LAP-based activation in cancer cells. On the other side, no remarkable phototoxicity or fluorescence turn-on was detected in healthy L929 cells. Thus, HCL serves as an effective and tumour associated LAP-sensitive phototheranostic agent. We believe different cancer-associated analytes can be utilized in combination with near-IR absorbing scaffolds in the scope of activatable PDT designs to enrich the tumour-selective PS arsenal.Publication Open Access A narrow-band multi-resonant metamaterial in near-ir(Multidisciplinary Digital Publishing Institute (MDPI), 2020) Ali, Farhan; Department of Physics; Ramazanoğlu, Serap Aksu; Faculty Member; Department of Physics; College of Sciences; 243745We theoretically investigate a multi-resonant plasmonic metamaterial perfect absorber operating between 600 and 950 nm wavelengths. The presented device generates 100% absorption at two resonance wavelengths and delivers an ultra-narrow band (sub-20 nm) and high quality factor (Q = 44) resonance. The studied perfect absorber is a metal–insulator–metal configuration where a thin MgF2 spacer is sandwiched between an optically thick gold layer and uniformly patterned gold circular nanodisc antennas. The localized and propagating nature of the plasmonic resonances are characterized and confirmed theoretically. The origin of the perfect absorption is investigated using the impedance matching and critical coupling phenomenon. We calculate the effective impedance of the perfect absorber and confirm the matching with the free space impedance. We also investigate the scattering properties of the top antenna layer and confirm the minimized reflection at resonance wavelengths by calculating the absorption and scattering cross sections. The excitation of plasmonic resonances boost the near-field intensity by three orders of magnitude which enhances the interaction between the metamaterial surface and the incident energy. The refractive index sensitivity of the perfect absorber could go as high as S = 500 nm/RIU. The presented optical characteristics make the proposed narrow-band multi-resonant perfect absorber a favorable platform for biosensing and contrast agent based bioimaging.Publication Metadata only Analysis of thermal fields in orthogonal machining with infrared imaging(Elsevier Science Sa, 2008) Department of Mechanical Engineering; Department of Mechanical Engineering; Department of Physics; Lazoğlu, İsmail; Serpengüzel, Ali; N/A; Faculty Member; Faculty Member; Department of Mechanical Engineering; Department of Physics; Manufacturing and Automation Research Center (MARC); Manufacturing and Automation Research Center (MARC); N/A; College of Engineering; College of Engineering; College of Sciences; N/A; 179391; 27855The validation of a previously developed finite difference temperature prediction model is carried out for orthogonal machining process with a high precision infrared camera set-up, considering the temperature distribution in the tool. the thermal experiments are conducted with two different materials; al 7075, AISI 1050, with two different tool geometries; inserts having a rake angle of 6 degrees and 18 degrees, for different cutting velocities and feedrates. an infrared camera set-up is utilized for the thermal experiments. the results of the high precision infrared thermal measurements are compared with the outputs of the finite difference temperature model, considering the maximum and the mean temperatures in the tool-chip interface zone and the temperature distributions on the tool take face. the maximum tool-chip interface temperature increases with increasing cutting velocity and feedrate. the relationship between the maximum tool-chip interface temperature and the rake angle of the tool is not distinctive. the experimental results show good agreement with the simulations. (c) 2007 Elsevier B.V. all rights reserved.Publication Metadata only Bypassing pro-survival and resistance mechanisms of autophagy in EGFR-positive lung cancer cells by targeted delivery of 5FU using theranostic Ag2S quantum dots(Royal Soc Chemistry, 2019) Akkoç, Yunus; Gözüaçık, Devrim; N/A; N/A; N/A; N/A; Department of Physics; Department of Chemistry; Demirci, Gözde; Duman, Fatma Demir; Bavili, Nima; Kiraz, Alper; Acar, Havva Funda Yağcı; PhD Student; Master Student; PhD Student; Faculty Member; Faculty Member; Department of Physics; Department of Chemistry; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; College of Sciences; School of Medicine; College of Sciences; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); N/A; N/A; N/A; 22542; 178902Targeted drug delivery systems that combine imaging and therapeutic functions in a single structure have become very popular in nanomedicine. Near-infrared (NIR) emitting Ag2S quantum dots (QDs) are excellent candidates for this task. Here, we have developed PEGylated Ag2S QDs functionalized with Cetuximab (Cet) antibody and loaded with an anticancer drug, 5-fluorouracil (5FU). These theranostic QDs were used for targeted NIR imaging and treatment of lung cancer using low (H1299) and high (A549) Epidermal Growth Factor Receptor (EGFR) overexpressing cell lines. The Cet conjugated QDs effectively and selectively delivered 5FU to A549 cells and provided significantly enhanced cell death associated with apoptosis. Interestingly, while treatment of cells with free 5FU activated autophagy, a cellular mechanism conferring resistance to cell death, these EGFR targeting multimodal QDs significantly overcame drug resistance compared to 5FU treatment alone. The improved therapeutic outcome of 5FU delivered to A549 cells by Cet conjugated Ag2S QDs is suggested as the synergistic outcome of enhanced receptor mediated uptake of nanoparticles, and hence the drug, coupled with suppressed autophagy even in the absence of addition of an autophagy suppressor.Publication Open Access Cadmium-free and efficient Type-II InP/ZnO/ZnS quantum dots and their application for LEDs(American Chemical Society (ACS), 2021) Ritter, Maximilian; Şahin, Mehmet; Ow-Yang, Cleva W.; Lechner, Rainer T.; Department of Electrical and Electronics Engineering; Department of Physics; N/A; N/A; Nizamoğlu, Sedat; Sennaroğlu, Alphan; Eren, Güncem Özgün; Sadeghi, Sadra; Jalali, Houman Bahmani; Han, Mertcan; Toker, Işınsu Baylam; Melikov, Rustamzhon; Önal, Asım; Öz, Fatma; Faculty Member; Faculty Member; PhD Student; PhD Student; Master Student; PhD Student; Department of Electrical and Electronics Engineering; Department of Physics; Koç University Surface Science and Technology Center (KUYTAM) / Koç Üniversitesi Yüzey Teknolojileri Araştırmaları Merkezi (KUYTAM); Koç University Boron and Advanced Materials Application and Research Center (KUBAM) / Koç Üniversitesi Bor ve İleri Malzemeler Uygulama ve Araştırma Merkezi (KUBAM); College of Engineering; College of Sciences; Graduate School of Sciences and Engineering; 130295; 23851; N/A; N/A; N/A; N/A; N/A; N/A; N/A; N/AIt is a generally accepted perspective that type-II nanocrystal quantum dots (QDs) have low quantum yield due to the separation of the electron and hole wavefunctions. Recently, high quantum yield levels were reported for cadmium-based typeII QDs. Hence, the quest for finding non-toxic and efficient type-II QDs is continuing. Herein, we demonstrate environmentally benign type-II InP/ZnO/ZnS core/shell/shell QDs that reach a high quantum yield of similar to 91%. For this, ZnO layer was grown on core InP QDs by thermal decomposition, which was followed by a ZnS layer via successive ionic layer adsorption. The small-angle Xray scattering shows that spherical InP core and InP/ZnO core/ shell QDs turn into elliptical particles with the growth of the ZnS shell. To conserve the quantum efficiency of QDs in device architectures, InP/ZnO/ZnS QDs were integrated in the liquid state on blue light-emitting diodes (LEDs) as down-converters that led to an external quantum efficiency of 9.4% and a power conversion efficiency of 6.8%, respectively, which is the most efficient QD-LED using type-II QDs. This study pointed out that cadmium-free type-II QDs can reach high efficiency levels, which can stimulate novel forms of devices and nanomaterials for bioimaging, display, and lighting.Publication Open Access Cetuximab-Ag2S quantum dots for fluorescence imaging and highly effective combination of ALA-based photodynamic/chemo-therapy of colorectal cancer cells(Royal Society of Chemistry (RSC), 2021) Mohammad Hadi, Layla; Yaghini, Elnaz; Loizidou, Marilena; MacRobert, Alexander J.; Department of Chemistry; N/A; Department of Physics; Acar, Havva Funda Yağcı; Bayır, Ali; Hashemkhani, Mahshid; Demirci, Gözde; Muti, Abdullah; Sennaroğlu, Alphan; Researcher; PhD Student; Master Student; PhD Student; Faculty Member; Department of Chemistry; Department of Physics; Koç University Surface Science and Technology Center (KUYTAM) / Koç Üniversitesi Yüzey Teknolojileri Araştırmaları Merkezi (KUYTAM); College of Sciences; Graduate School of Sciences and Engineering; 178902; N/A; N/A; N/A; N/A; 23851Colorectal cancer (CRC) has a poor prognosis and urgently needs better therapeutic approaches. 5-Aminolevulinic acid (ALA) induced protoporphyrin IX (PpIX) based photodynamic therapy (PDT) is already used in the clinic for several cancers but not yet well investigated for CRC. Currently, systemic administration of ALA offers a limited degree of tumour selectivity, except for intracranial tumours, limiting its wider use in the clinic. The combination of effective ALA-PDT and chemotherapy may provide a promising alternative approach for CRC treatment. Herein, theranostic Ag2S quantum dots (AS-2MPA) optically trackable in near-infrared (NIR), conjugated with endothelial growth factor receptor (EGFR) targeting Cetuximab (Cet) and loaded with ALA for PDT monotherapy or ALA/5-fluorouracil (5FU) for the combination therapy are proposed for enhanced treatment of EGFR(+) CRC. AS-2MPA-Cet exhibited excellent targeting of the high EGFR expressing cells and showed a strong intracellular signal for NIR optical detection in a comparative study performed on SW480, HCT116, and HT29 cells, which exhibit high, medium and low EGFR expression, respectively. Targeting provided enhanced uptake of the ALA loaded nanoparticles by strong EGFR expressing cells and formation of higher levels of PpIX. Cells also differ in their efficiency to convert ALA to PpIX, and SW480 was the best, followed by HT29, while HCT116 was determined as unsuitable for ALA-PDT. The therapeutic efficacy was evaluated in 2D cell cultures and 3D spheroids of SW480 and HT29 cells using AS-2MPA with either electrostatically loaded, hydrazone or amide linked ALA to achieve different levels of pH or enzyme sensitive release. Most effective phototoxicity was observed in SW480 cells using AS-2MPA-ALA-electrostatic-Cet due to enhanced uptake of the particles, fast ALA release and effective ALA-to-PpIX conversion. Targeted delivery reduced the effective ALA concentration significantly which was further reduced with codelivery of 5FU. Delivery of ALA via covalent linkages was also effective for PDT, but required a longer incubation time for the release of ALA in therapeutic doses. Phototoxicity was correlated with high levels of reactive oxygen species (ROS) and apoptotic/necrotic cell death. Hence, both AS-2MPA-ALA-Cet based PDT and AS-2MPA-ALA-Cet-5FU based chemo/PDT combination therapy coupled with strong NIR tracking of the nanoparticles demonstrate an exceptional therapeutic effect on CRC cells and excellent potential for synergistic multistage tumour targeting therapy.Publication Metadata only Cooper pairing, flat-band superconductivity, and quantum geometry in the pyrochlore-Hubbard model(American Physical Society, 2024) ; Department of Physics; Işkın, Menderes; Department of Physics; ; College of Sciences;We investigate the impacts of the quantum geometry of Bloch states, specifically through the band -resolved quantum -metric tensor, on Cooper pairing and flat -band superconductivity in a three-dimensional pyrochloreHubbard model. First we analyze the low-lying two -body spectrum exactly, and show that the pairing order parameter is uniform in this four -band lattice. This allows us to establish direct relations between the superfluid weight of a multiband superconductor and (i) the effective mass of the lowest -lying two -body branch at zero temperature, (ii) the kinetic coefficient of the Ginzburg-Landau theory in proximity to the critical temperature, and (iii) the velocity of the low -energy Goldstone modes at zero temperature. Furthermore, we perform a comprehensive numerical analysis of the superfluid weight and Goldstone modes, exploring both their conventional and geometric components at zero temperature.Publication Metadata only Cyto/hemocompatible magnetic hybrid nanoparticles (ag2s-fe3o4) with luminescence in the near-infrared region as promising theranostic materials(Elsevier, 2015) Grandfils, Christian; Ojea-Jimenez, Isaac; Rossi, Francois; Dogan, Nurcan; Department of Physics; N/A; Department of Chemistry; Department of Chemistry; N/A; Kiraz, Alper; Hocaoğlu, İbrahim; Aşık, Didar; Acar, Havva Funda Yağcı; Ulusoy, Gülen; Faculty Member; PhD Student; Master Student; Faculty Member; N/A; Department of Physics; Department of Chemistry; College of Sciences; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; College of Sciences; N/A; 22542; N/A; N/A; 178902; N/ASmall hybrid nanoparticles composed of highly biocompatible Ag2S quantum dots (QD) emitting in the near-infrared region and superparamagnetic iron oxide (SPION) are produced in a simple extraction method utilizing ligand exchange mechanism. Hybrid nanoparticles luminesce at the same wavelength as the parent QD, therefore an array of hybrid nanoparticles with emission between 840 and 912 nm were easily produced. Such hybrid structures have (1) strong luminescence in the medical imaging window eliminating the autofluoresence of cells as effective optical probes, (2) strong magnetic response for magnetic targeting and (3) good cyto/hemocompatibility. An interesting size dependent cytotoxicity behavior was observed in HeLa and NIH/3T3 cell lines: smallest particles are internalized significantly more by both of the cell lines, yet showed almost no significant cytotoxicity in HeLa between 10 and 25 mu g/mL Ag concentration but were most toxic in NIH/3T3 cells. Cell internalization and hence the cytotoxicity enhanced when cells were incubated with the hybrid nanoparticles under magnetic field, especially with the hybrid nanoparticles containing larger amounts of SPION in the hybrid composition. These results prove them as effective optical imaging agents and magnetic delivery vehicles. Combined with the known advantages of SPIONs as a contrast agent in MRI, these particles are a step forward for new theranostics for multimode imaging and magnetic targeting.Publication Metadata only Elastic constants of a Laves phase compound: C15 NbCr2(Materials Research Society, 1997) Chu, Fredy; Wills, Josephine M.; Chen, S. P.; Albers, Robert C; Thoma, Dan J.; Mitchell, Thomas Elliot; Department of Physics; Örmeci, Alim; Researcher; Department of Physics; College of Sciences; N/AThe single-crystal elastic constants of C15 NbCr2 have been computed by using a first-principles, self-consistent, full-potential total energy method. From these single-crystal elastic constants the isotropic elastic moduli are calculated using the Voigt and Reuss averages. The calculated values are in fair agreement with the experimental values. The implications of the results are discussed with regards to Poisson's ratio and the direction dependence of Young's modulus.Publication Metadata only Enhanced dissolution of liquid microdroplets in the extensional creeping flow of a hydrodynamic trap(Amer Chemical Soc, 2016) Tanyeri, Melikhan; N/A; Department of Physics; N/A; N/A; N/A; N/A; N/A; Department of Mechanical Engineering; Department of Physics; Mustafa, Adil; Erten, Ahmet Can; Ayaz, Rana Muhammed Armaghan; Kayıllıoğlu, Oğuz; Eser, Ayşenur; Eryürek, Mustafa; Irfan, Muhammad; Muradoğlu, Metin; Kiraz, Alper; PhD Student; Teaching Faculty; PhD Student; PhD Student; Master Student; PhD Student; PhD Student; Faculty Member; Faculty Member; Department of Mechanical Engineering; Department of Physics; Graduate School of Sciences and Engineering; College of Sciences; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; Graduate School of Sciences and Engineering; College of Engineering; College of Sciences; N/A; 233923; N/A; N/A; N/A; N/A; N/A; 46561; 22542A novel noncontact technique based on hydrodynamic trapping is presented to study the dissolution of freely suspended liquid microdroplets into a second immiscible phase in a simple extensional creeping flow. Benzyl benzoate (BB) and n-decanol microdroplets are individually trapped at the stagnation point of a planar extensional flow, and dissolution of single microdroplets into an aqueous solution containing surfactant is characterized at different flow rates. The experimental dissolution curves are compared to two models: (i) the Epstein-Plesset (EP) model which considers only diffusive mass transfer, and (ii) the Zhang-Yang-Mao (ZYM) model which considers both diffusive and convective mass transfer in the presence of extensional creeping flow. The EP model significantly underpredicts the experimentally determined dissolution rates for all experiments. In contrast, very good agreement is observed between the experimental dissolution curves and the ZYM model when the saturation concentration of the microdroplet liquid (c(s)) is used as the only fitting parameter. Experiments with BB microdroplets at low surfactant concentration (10 mu M) reveal c(s) values very similar to that reported in the literature. In contrast, experiments with BB and n-decanol microdroplets at 10 mM surfactant concentration, higher than the critical micelle concentration (CMC) of 5 mM, show further enhancements in microdroplet dissolution rates due to micellar solubilization. The presented method accurately tests the dissolution of single microdroplets into a second immiscible phase in extensional creeping flow and has potential for applications such as separation processes, food dispersion, and drug development/design.