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Publication Metadata only 3D printed kombucha biomaterial as a tissue scaffold and L929 cell cytotoxicity assay(Wiley, 2024) Yanbakan, Edaguel; Tuncel, Tugba; Kocak Sezgin, Ayse; Bozoglan, Emirhan; Berikten, Derya; Kar, Fatih; Department of Molecular Biology and Genetics; Bağlan, İlkyaz; Department of Molecular Biology and Genetics; College of SciencesTissue engineering includes the construction of tissue-organ scaffold. The advantage of three-dimensional scaffolds over two-dimensional scaffolds is that they provide homeostasis for a longer time. The microbial community in Symbiotic culture of bacteria and yeast (SCOBY) can be a source for kombucha (kombu tea) production. In this study, it was aimed to investigate the usage of SCOBY, which produces bacterial cellulose, as a biomaterial and 3D scaffold material. 3D printable biomaterial was obtained by partial hydrolysis of oolong tea and black tea kombucha biofilms. In order to investigate the usage of 3D kombucha biomaterial as a tissue scaffold, "L929 cell line 3D cell culture" was created and cell viability was tested in the biomaterial. At the end of the 21st day, black tea showed 51% and oolong tea 73% viability. The cytotoxicity of the materials prepared by lyophilizing oolong and black tea kombucha beverages in fibroblast cell culture was determined. Black tea IC50 value: 7.53 mg, oolong tea IC50 value is found as 6.05 mg. Fibroblast viability in 3D biomaterial + lyophilized oolong and black tea kombucha beverages, which were created using the amounts determined to these values, were investigated by cell culture Fibroblasts in lyophilized and 3D biomaterial showed viability of 58% in black tea and 78% in oolong tea at the end of the 7th day. In SEM analysis, it was concluded that fibroblast cells created adhesion to the biomaterial. 3D biomaterial from kombucha mushroom culture can be used as tissue scaffold and biomaterial.Publication Metadata only A fatal progeroid syndrome caused by a recessive RAF1 loss-of-function mutation(Springernature, 2023) Wong, Samantha; Tan, Yu Xuan; Tan, Kiat Yi; Loh, Abigail; Ozkan, Engin; Kayserili, Hülya; Reversade, Bruno; Beillard, Nathalie Sonia Escande; School of Medicine; Koç University HospitalPublication Metadata only A low cost training phantom model for radio-guided localization techniques in occult breast lesions(Wiley, 2015) Aydoğan, Fatih; Mallory, Melissa Anne; Tükenmez, Mustafa; Sagara, Yasuaki; Özturk, Erkan; Çelik, Varol; Akça, Tamer; Golshan, Mehra; N/A; İnce, Hüseyin Yavuz; Faculty Member; School of Medicine; N/ARadio-guided localization (RGL) for identifying occult breast lesions has been widely accepted as an alternative technique to other localization methods, including those using wire guidance. An appropriate phantom model would be an invaluable tool for practitioners interested in learning the technique of RGL prior to clinical application. The aim of this study was to devise an inexpensive and reproducible training phantom model for RGL. We developed a simple RGL phantom model imitating an occult breast lesion from inexpensive supplies including a pimento olive, a green pea and a Turkey breast. The phantom was constructed for a total cost of less than $20 and prepared in approximately 10 min. After the first model's construction, we constructed approximately 25 additional models and demonstrated that the model design was easily reproducible. The RGL phantom is a time- and cost-effective model that accurately simulates the RGL technique for non-palpable breast lesions. Future studies are warranted to further validate this model as an effective teaching tool. J. Surg. Oncol. 2015; 112:449-451. © 2015 Wiley Periodicals, Inc.Publication Metadata only A multicenter, retrospective archive study of radiological and clinical features of ALK-positive non-small cell lung cancer patients and crizotinib efficacy(Lippincott Williams and Wilkins, 2024) Kilickap, Saadettin; Ozturk, Akin; Karadurmus, Nuri; Korkmaz, Taner; Cicin, Irfan; Paydas, Semra; Cilbir, Ebru; Sakalar, Teoman; Uysal, Mukremin; Yesil Cinkir, Havva; Uskent, Necdet; Demir, Necla; Sakin, Abdullah; Dursun, Oldac Uras; Aver, Birkan; Turhal, Nazim Serdar; Keskin, Serkan; Tural, Deniz; Eralp, Yesim; Bugdayci Basal, Fatma; Yasar, Hatime Arzu; Sendur, Mehmet Ali Nahit; Demirci, Umut; Cubukcu, Erdem; Karaagac, Mustafa; Cakar, Burcu; Tatli, Ali Murat; Yetisyigit, Tarkan; Urvay, Semiha; Gursoy, Pinar; Oyan, Basak; Turna, Zeynep Hande; Isikdogan, Abdurrahman; Olmez, Omer Fatih; Yazici, Ozan; Cabuk, Devrim; Seker, Mehmet Metin; Unal, Olcun Umit; Meydan, Nezih; Okutur, Sadi Kerem; Erman, Mustafa; Yumuk, Perran Fulden; Tunalı, Didem; School of MedicineTo evaluate radiological and clinical features in metastatic anaplastic lymphoma kinase+ non-small cell lung cancer patients and crizotinib efficacy in different lines. This national, non-interventional, multicenter, retrospective archive screening study evaluated demographic, clinical, and radiological imaging features, and treatment approaches in patients treated between 2013-2017. Totally 367 patients (54.8% males, median age at diagnosis 54 years) were included. Of them, 45.4% were smokers, and 8.7% had a family history of lung cancer. On radiological findings, 55.9% of the tumors were located peripherally, 7.7% of the patients had cavitary lesions, and 42.9% presented with pleural effusion. Pleural effusion was higher in nonsmokers than in smokers (37.3% vs. 25.3%, P = .018). About 47.4% of cases developed distant metastases during treatment, most frequently to the brain (26.2%). Chemotherapy was the first line treatment in 55.0%. Objective response rate was 61.9% (complete response: 7.6%; partial response: 54.2%). The highest complete and partial response rates were observed in patients who received crizotinib as the 2nd line treatment. The median progression-free survival was 14 months (standard error: 1.4, 95% confidence interval: 11.2-16.8 months). Crizotinib treatment lines yielded similar progression-free survival (P = .078). The most frequent treatment-related adverse event was fatigue (14.7%). Adrenal gland metastasis was significantly higher in males and smokers, and pleural involvement and effusion were significantly higher in nonsmokers-a novel finding that has not been reported previously. The radiological and histological characteristics were consistent with the literature data, but several differences in clinical characteristics might be related to population characteristics.Publication Metadata only A progeroid syndrome with severe osteogenesis imperfecta segregates with an intronic TAPT1 homozygous variant that creates a knockout allele(Springernature, 2023) Bressin, Annkatrin; Chia, PohHui; Traspas, Ricardo Moreno; Bonnard, Carine; Hojat, Zohreh; Drutman, Scott; Casanova, Jean-Laurent; Shboul, Mohammad; Mayor, Andreas; Nabavizadeh, Nasrinsadat; Beillard, Nathalie Sonia Escande; Reversade, Bruno; School of MedicinePublication Metadata only A retrospective cross-sectional analysis of familial adenomatous polyposis coli cases(Springernature, 2023) Yılmaz, Elanur; Börklü Yücel, Esra; Saraçoğlu, Hilal Pırıl; Balık, Emre; Avcı, Şahin; Eraslan, Serpil; Buğra, Dursun; Kayserili, Hülya; Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM); Graduate School of Health Sciences; School of Medicine; Koç University HospitalPublication Open Access A telerehabilitation approach to chronic facial paralysis in the COVID-19 pandemic scenario: what role for electromyography assessment?(Multidisciplinary Digital Publishing Institute (MDPI), 2022) de Sire A., Marotta N., Agostini F., Drago Ferrante V., Demeco A., Ferrillo M., Inzitari M.T., Pellegrino R., Russo I., Taskiran O.O., Bernetti A., Ammendolia A.; Taşkıran, Özden Özyemişçi; Faculty Member; School of Medicine; 133091There is a lack of data on patient and diagnostic factors for prognostication of complete recovery in patients with peripheral facial palsy. Thus, the aim of this study was to evaluate the role of a telerehabilitave enhancement through the description of a case report with the use of short-wave diathermy and neuromuscular electrical stimulation combined to facial proprioceptive neuromuscular facilitation (PNF) rehabilitation in unrecovered facial palsy, in a COVID-19 pandemic scenario describing a paradigmatic telerehabilitation report. A 43-year-old woman underwent a facial rehabilitation plan consisting of a synergistic treatment with facial PNF rehabilitation, short-wave diathermy, and neuromuscular electrical stimulation (12 sessions lasting 45 min, three sessions/week for 4 weeks). Concerning the surface electromyography evaluation of frontal and orbicularis oris muscles, the calculated ratio between amplitude of the palsy side and normal side showed an improvement in terms of movement symmetry. At the end of the outpatient treatment, a daily telere-habilitation protocol with video and teleconsultation was provided, showing a further improvement in the functioning of a woman suffering from unresolved facial paralysis. Therefore, an adequate telerehabilitation follow-up seems to play a fundamental role in the management of patients with facial palsy.Publication Metadata only A Turkish 3-center study evaluation of serum folic acid and vitamin B12 levels in Alzheimer disease(Scientific and Technical Research Council Turkey (TUBITAK), 2015) Yılmaz, Gökhan; Erbayraktar, Zübeyde; Evlice, Ahmet Turan; Aras, Sevgi; Yener, Görsev; Avcı, Aslıhan; Ulusu, Nuriye Nuray; Faculty Member; School of Medicine; 6807Background/Aim: Alzheimer disease, a common proteopathy of advanced age, is characterized by cortical atrophy, neuron degeneration, neuronal loss, and accumulation of extracellular amyloid beta plaques. We aimed to investigate serum vitamin B12 and folic acid levels in Alzheimer disease and other dementia patients, as a potential screening test to detect presymptomatic Alzheimer disease in Turkish patients. Materials and Methods: We evaluated folic acid and vitamin B12 levels in Alzheimer disease patients as well as in other dementia and geriatric patients from Ankara, Dokuz Eylul, and Cukurova university hospitals; 290 female and male geriatric subjects were enrolled. Vitamin B12 and folic acid levels were measured using Roche E170 and Beckman Coulter DXI 800 immunoassays (chemiluminescence) according to the manufacturers' guideline in all centers. Results: We evaluated the results of folic acid and vitamin B12 in Alzheimer disease, other dementias and geriatric patients. No significant difference between the groups regarding the routine control of biochemical parameters was observed. Conclusion: Currently, serum folic and vitamin B12 levels are not diagnostically reliable tests for screening presymptomatic Alzheimer disease. However, the results may statistically be significant if we increase the sample size.Publication Metadata only Additive manufacturing and three-dimensional printing in obstetrics and gynecology: a comprehensive review(Springer Heidelberg, 2023) Dabbagh, Sajjad Rahmani; Aydın, Serdar; KU Arçelik Research Center for Creative Industries (KUAR) / KU Arçelik Yaratıcı Endüstriler Uygulama ve Araştırma Merkezi (KUAR); Koç Üniversitesi İş Bankası Yapay Zeka Uygulama ve Araştırma Merkezi (KUIS AI)/ Koç University İş Bank Artificial Intelligence Center (KUIS AI); School of MedicinePublication Metadata only Advancing language concordant care: a multimodal medical interpretation intervention(BMJ Publishing Group, 2024) Sharfuddin, Nazia; Mathura, Pamela; Mac, Amanda; Ling, Emily; Khatib, Emad; Suranyi, Yvonne; Kassam, Narmin; Tan, Merve; School of MedicineBackground Ensuring language concordant care through medical interpretation services (MIS) allows for accurate information sharing and positive healthcare experiences. The COVID-19 pandemic led to a regional halt of in-person interpreters, leaving only digital MIS options, such as phone and video. Due to longstanding institutional practices, and lack of accessibility and awareness of these options, digital MIS remained underused. A Multimodal Medical Interpretation Intervention (MMII) was developed and piloted to increase digital MIS usage by 25% over an 18-month intervention period for patients with limited English proficiency.Methods Applying quality improvement methodology, an intervention comprised digital MIS technology and education was trialled for 18 months. To assess intervention impact, the number of digital MIS minutes was measured monthly and compared before and after implementation. A questionnaire was developed and administered to determine healthcare providers' awareness, technology accessibility and perception of MIS integration in the clinical workflow.Results Digital MIS was used consistently from the beginning of the COVID-19 pandemic (March 2020) and over the subsequent 18 months. The total number of minutes of MIS use per month increased by 44% following implementation of our intervention. Healthcare providers indicated that digital MIS was vital in facilitating transparent communication with patients, and the MMII ensured awareness of and accessibility to the various MIS modalities.Conclusion Implementation of the MMII allowed for an increase in digital MIS use in a hospital setting. Providing digital MIS access, education and training is a means to advance patient-centred and equitable care by improving accuracy of clinical assessments and communication.