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Publication Metadata only A comparison of the survival and implantation rates of blastocysts that were vitrified on postfertilization day five, six and seven(Taylor & Francis Ltd, 2019) Berrin, Avci; Isil, Kasapoglu; Goktan, Kuspinar; Seda, Saribal; Gurkan, Uncu; N/A; Ata, Mustafa Barış; Faculty Member; School of Medicine; 182910The goal of this retrospective cohort study was to compare survival, implantation, clinical and ongoing pregnancy rates between blastocysts that were vitrified on post-fertilization days 5, 6 and 7. Before vitrification, blastocysts were evaluated in terms of morphology and blastocyst expansion, inner cell mass and trophectoderm quality. They were thawed and transfered in a subsequent artificial cycle. Embryo implantation rates were 39%, 25% and 25% for blastocysts that were vitrified on days 5, 6, and 7, respectively (p = 0.006). Clinical and ongoing pregnancy rates were 19%, 12%, 13% (p = 0.100) and 9%, 7%, 12% (p = 0.99) for days 5, 6 and 7 blastocysts, respectively. Day 5 blastocysts had significantly higher full-collapsing score after assisted-hatching compared to days 6 and 7 blastocysts (p = 0.014). As blastocyst quality increased, implantation and clinical pregnancy rates increased in all groups and both parameters were statistically significantly higher on day 5 blastocysts than on days 6 or 7 (p = 0.001). It was clearly found that good quality blastocysts obtained on day 5 have higher implantation and clinical pregnancy rates than 6th and 7th day cryopreserved embryos. There were no statistically significant differences between the cryopreserved embryos on days 6 and 7 regarding the implantation, clinic and ongoing pregnancy rates.Publication Open Access A new definition of recurrent implantation failure on the basis of anticipated blastocyst aneuploidy rates across female age(Elsevier, 2021) Somigliana, Edgardo; Ata, Mustafa Barış; Kalafat, Erkan; Faculty Member; Faculty Member; School of Medicine; Koç University Hospital; 182910; 197389Objective: to present a definition of recurrent implantation failure that accounts for the effects of female age and anticipated blastocyst euploidy rates on cumulative implantation rates. Design: mathematical modeling. Setting: not applicable. Patient(s): not applicable. Intervention(s): mathematical modeling of cumulative implantation probability on the basis of published blastocyst euploidy rates across categories of female age. Main Outcome Measure(s): the number of blastocysts required to achieve 95% cumulative implantation probability under the assumption of the absence of any other factor affecting implantation. Result(s): when the euploidy status of the transferred embryo is unknown (i.e., not subjected to preimplantation genetic testing for aneuploidies), our simulation shows that no age category reaches 95% cumulative probability of implantation of at least one embryo until after transfer of seven blastocysts. The number of blastocysts required to reach the same threshold is higher for older patients. For example, women older than 38 years require transfer of more than 10 untested blastocysts for the upper range of predictive probability to meet the threshold of 95%. On the other hand, if the implantation rate for a euploid blastocyst is assumed to be 55%, then 4 blastocysts are enough to reach a cumulative probability rate greater than 95%, regardless of age. Conclusion(s): the term ""recurrent implantation failure""should be a functional term guiding further management. We suggest that recurrent implantation failure should not be called until implantation failure becomes reasonably likely to be caused by factors other than embryo aneuploidy, the leading cause of implantation failure. We propose a new definition that factors in anticipated blastocyst euploidy rates across categories of female age, euploid blastocyst implantation rate, and a specified threshold of cumulative probability of implantation. / Objetivo: Presentar una definicion de fallo recurrente de implantacion que tenga en cuenta los efectos de la edad de la mujer y las tasasanticipadas de euploidia de blastocisto sobre las tasas acumulativas de implantacion. Dise no: modelo matematico. Lugar: no aplicable. Pacientes(s): no aplicable. Intervencion(es): modelo matematico de probabilidad acumulativa de implantacion basado en las tasas de euploidia de blastocistopublicadas segun categorias de edad de la mujer. Principal(es) medida(s) de resultado(s): el numero de blastocistos requerido para obtener una probabilidad acumulativa de im-plantacion del 95% asumiendo la ausencia de cualquier otro factor que afecte la implantacion. Resultado(s): cuando es estado de euploidia del embrion transferido es desconocido (i.e., no sujeto a diagnostico genetico preimplan-tacional para aneuploidias), nuestra simulacion demuestra que ninguna categoria de edad llega al 95% de probabilidad acumulativa deimplantacion de al menos un embrion hasta despues de transferir siete blastocistos. El numero de blastocistos requeridos para alcanzarel mismo nivel es mayor en pacientes mayores. Por ejemplo, las mujeres mayores de 38 anos requieren transferencia de mas de 10 blas-tocistos sin evaluar para que el rango superior de probabilidad predictiva alcance el nivel de 95%. Por otra parte, si la tasa de im-plantacion de un blastocisto euploide se estima en 55%, entonces 4 blastocistos son suficientes para alcanzar una tasa deprobabilidad acumulativa mayor del 95%, sin importar la edad. Conclusion(es): el termino ‘‘fallo recurrente de implantacion’’debería ser un termino funcional para guiar el manejo posterior. Suger-imos que no deberia llamarse fallo recurrente de implantacion hasta que sea razonablemente probable que el fallo de implantacion seacausado por otros factores ademas de la aneuploidía embrionaria, la principal causa de fallo de implantacion. Proponemos una nuevadefinicion que tenga en cuenta las tasas anticipadas de euploidia de blastocistos segun categorias de edad de la mujer, tasa de im-plantacion de blastocisto euploide y un nivel especifico de probabilidad acumulativa de implantacion.Publication Metadata only A novel flexible progestin primed ovarian stimulation protocol: comparison of pregnancy outcomes with the flexible GnRH antagonist protocol in an oocyte donation program(Elsevier, 2019) Eraslan, Alper; Angun, Berk; N/A; Yıldız, Şule; Türkgeldi, Engin; Ata, Mustafa Barış; Faculty Member; Faculty Member; Faculty Member; School of Medicine; School of Medicine; School of Medicine; Koç University Hospital; Koç University Hospital; N/A; 134205; 329649; 232576Publication Metadata only Activin-a promotes luteal regression by down-regulating the expression of steroidogenic enzymes and up-regulating BMP-6 and activin-A subunit in human luteal granulosa cells(Elsevier, 2015) Güzel, Yılmaz; Seyhan Ata, Ayşe; Balaban, Başak; N/A; Akın, Nazlı; Bildik, Gamze; Urman, Cumhur Bülent; Öktem, Özgür; Master Student; Master Student Faculty Member; Faculty Member; Graduate School of Health Sciences; Graduate School of Health Sciences; School of Medicine; School of Medicine; N/A; N/A; 12147; 102627Publication Open Access An example from the rural areas of Turkey: women breast cancer risk levels and application and knowledge regarding early diagnosis-scan of breast cancer(Aves, 2017) Türk, Rukiye; Terzioglu, Füsun; Taşkın, Lale; N/A; Eroğlu, Kafiye; Faculty Member; School of Nursing; 6061Objective: This research has been conducted for the purpose of determining the cancer risk levels of women living in a small village of in Saraycık village of Ankara and their knowledge and application of breast cancer early diagnose-scan methods. Materials and Methods: 317 women were taken as examples for the study. Data were collected by giving survey forms to women and conducting face-to-face interviews. In determining breast cancer risk, ''the form to determine the breast cancer risk'' has been used. For breast cancer informational questions, one point has been given for each correct answer. In evaluating the data, number, percentage calculations, average and standard deviation, Mann-Whitney U (MU), Kruskal-Wallis (KW), One-way analysis of variance (F) independent sample T (t) tests have been used. Results: It has been found that breast cancer risk is low, the knowledge level about cancer early recognition methods are medium among the women. It has been determined that 74.4% women didn't perform breast self-examination. 89.6% of women don't have mammography taken and 88.6% don't have their breast examined by health personnel. Conclusion: In our study, it has been found that the risk levels of women are low, their knowledge about early diagnosis and cure are at a medium level and their use of these methods are inadequate. For this reason, we suggest that responsibility of healthcare professionals have to be increased in determining breast cancer risk among women and education and advisory services for this subject to be offered.Publication Metadata only Application of seminal plasma to female genital tract prior to embryo transfer in assisted reproductive technology cycles (IVF, ICSI and frozen embryo transfer)(Oxford Univ Press, 2018) Abou Setta, A.; Seyhan, A.; Buckett, W.; N/A; Ata, Mustafa Barış; Faculty Member; School of Medicine; 232576N/APublication Metadata only c-Abl is not activated in genomic damage induced and TAp63 mediated oocyte apoptosis in human(Elsevier, 2018) N/A; N/A; Öktem, Özgür; Bildik, Gamze; Yakın, Kayhan; Urman, Cumhur Bülent; Faculty Member; Teaching Faculty; Faculty Member; Faculty Member; School of Medicine; Graduate School of Health Sciences; School of Medicine; School of Medicine; 102627; N/A; 106822; 12147There is a controversy in literature as to whether c-Abl is crucial for the induction of TAp63-mediated apoptosis and whether that inhibition of c-Abl with imatinib, which was designed to inhibit the oncogenic kinase BCR-ABL and c-kit, protects oocytes from chemotherapy-induced apoptosis in mice. No human data are available on this issue. We therefore aimed to explore whether genomic damage induced by chemotherapy drug cisplatin activates c-Abl along with TAp63 and the inhibition of c-Abl with imatinib prevents cisplatin-induced oocyte death and follicle loss in human ovary. Exposure to cisplatin induced DNA damage, activated TAp63 and SAPK/JNK pathway, and triggered apoptosis in the oocytes and granulosa cells. However, TAp63 activation after cisplatin was not associated with any increase in the expression of c-Abl. Imatinib did not prevent cisplatin-induced apoptosis of the granulosa cells or oocytes. Moreover, treatment with this drug resulted in the formation of bizarre shaped follicles lacking oocytes and increased follicular atresia by inducing apoptosis of granulosa cells and oocytes. Similar toxic effects were observed when ovarian tissue samples were incubated with a c-kit antagonist drug anti-CD117, but not with another c-Abl tyrosine kinase inhibitor GNF-2, which lacks an inhibitory action on c-kit. Intraperitoneal administration of imatinib to the xenografted animals produced similar histomorphological abnormalities in the follicles in human ovarian grafts and did not prevent cisplatin-induced follicle loss when co-administered with cisplatin. Our findings provide, for the first time, a molecular evidence for ovarian toxicity of this drug in human. Furthermore, this study together with two previous case reports of a severely compromised ovarian response to gonadotropin stimulation and premature ovarian failure in patients, while receiving imatinib, further heighten the concerns about its potential gonadotoxicity on human ovary and urge caution in its use in young female patients.Publication Metadata only Comparison of different progestin regimens for pituitary suppression during ovarian stimulation for assisted reproductive technology, a systematic review(Oxford Univ Press, 2020) N/A; N/A; N/A; N/A; N/A; Ata, Mustafa Barış; Türkgeldi, Engin; Alexandru, Polexa; Çekiç, Sebile Güler; Yıldız, Şule; Faculty Member; Faculty Member; Other; Doctor; Faculty Member; School of Medicine; School of Medicine; N/A; N/A; School of Medicine; N/A; Koç University Hospital; Koç University Hospital; Koç University Hospital; N/A; 182910; 329649; N/A; N/A; 134205N/APublication Metadata only Comparison of survival and implantation rates of blastocysts that were vitrified on post-fertilization day 5, 6 or 7(Elsevier Science Inc, 2015) Saribal, S.; Uncu, G.; Avcı, B.; N/A; Ata, Mustafa Barış; Faculty Member; School of Medicine; 182910N/APublication Metadata only Correct assessment and interpretation of results determines the accuracy of any diagnostic test, and PGT-A is no exception(Oxford Univ Press, 2022) Popovich, Mina; Fatemi, Human; N/A; Ata, Mustafa Barış; Faculty Member; School of Medicine; 182910N/A