Researcher:
Birinci, Yelda

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Researcher

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Yelda

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Birinci

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Birinci, Yelda
Birinci Kudu, Yelda

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2020 - 20223

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Researcher Of Publications: search.filters.isAuthorOfPublication.73eaef25-de81-4eb7-86ef-19a2ea0df7dd

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    Role of exosomal MicroRNAs in cell-to-cell communication
    (Humana Press Inc., 2022) Tastan, Bora; Tarakcioglu, Emre; Genc, Sermin; Department of Molecular Biology and Genetics; Department of Molecular Biology and Genetics; Park, Yongsoo; Birinci, Yelda; Faculty Member; Researcher; Department of Molecular Biology and Genetics; College of Sciences; College of Sciences; 240759; 183492
    Exosomes, a type of extracellular vesicle, are small vesicles (30–100 nm) secreted into extracellular space from almost all types of cells. Exosomes mediate cell-to-cell communication carrying various biologically active molecules including microRNAs. Studies have shown that exosomal microRNAs play fundamental roles in healthy and pathological conditions such as immunity, cancer, and inflammation. In this chapter, we introduce the current knowledge on exosome biogenesis, techniques used in exosome research, and exosomal miRNA and their functions in biological and pathological processes.
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    Author correction: isolation of large dense-core vesicles from bovine adrenal medulla for functional studies (Scientific Reports, (2020), 10, 1, (7540), 10.1038/s41598-020-64486-3)
    (Springer Nature, 2022) Preobraschenski, Julia; Ganzella, Marcelo; Jahn, Reinhard; Park, Yongsoo; Department of Molecular Biology and Genetics; Birinci, Yelda; Researcher; Department of Molecular Biology and Genetics; College of Sciences; 183492
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    PublicationOpen Access
    Isolation of large dense-core vesicles from bovine adrenal medulla for functional studies
    (Nature Publishing Group (NPG), 2020) Preobraschenski, Julia; Ganzella, Marcelo; Jahn, Reinhard; Department of Molecular Biology and Genetics; Birinci, Yelda; Park, Yongsoo; Faculty Member; Department of Molecular Biology and Genetics; College of Sciences; 183492; 240759
    Large dense-core vesicles (LDCVs) contain a variety of neurotransmitters, proteins, and hormones such as biogenic amines and peptides, together with microRNAs (miRNAs). Isolation of LDCVs is essential for functional studies including vesicle fusion, vesicle acidification, monoamine transport, and the miRNAs stored in LDCVs. Although several methods were reported for purifying LDCVs, the final fractions are significantly contaminated by other organelles, compromising biochemical characterization. Here we isolated LDCVs (chromaffin granules) with high yield and purity from bovine adrenal medulla. The fractionation protocol combines differential and continuous sucrose gradient centrifugation, allowing for reducing major contaminants such as mitochondria. Purified LDCVs show robust acidification by the endogenous V-ATPase and undergo SNARE-mediated fusion with artificial membranes. Interestingly, LDCVs contain specific miRNAs such as miR-375 and miR-375 is stabilized by protein complex against RNase A. This protocol can be useful in research on the biological functions of LDCVs.