Publication: Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2
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Zeng, Ruizhu
Sidik, Harwin
Robinson, Kim S.
Zhong, Franklin L.
Pouladiahi, Mahmoud A.
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Abstract
Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.
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Elsevier
Subject
Medicine
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Source
Stem Cell Research
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DOI
10.1016/j.scr.2019.101533