Publication: Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2
Files
Program
KU-Authors
KU Authors
Co-Authors
Zeng, Ruizhu
Sidik, Harwin
Robinson, Kim S.
Zhong, Franklin L.
Pouladiahi, Mahmoud A.
Publication Date
Language
Type
Embargo Status
NO
Journal Title
Journal ISSN
Volume Title
Alternative Title
Abstract
Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.
Source
Publisher
Elsevier
Subject
Medicine
Citation
Has Part
Source
Stem Cell Research
Book Series Title
Edition
DOI
10.1016/j.scr.2019.101533