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Transgene-free disease-specific iPSC generation from fibroblasts and peripheral blood mononuclear cells

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dc.contributor.coauthorN/A
dc.contributor.departmentSchool of Medicine
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.facultymemberYes
dc.contributor.kuauthorFidan, Kerem
dc.contributor.kuauthorEbrahimi, Ayyub A.
dc.contributor.kuauthorBozlak, Özlem Hilal Çağlayan
dc.contributor.kuauthorÖzçimen, Burcu
dc.contributor.kuauthorÖnder, Tamer Tevfik
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T22:55:59Z
dc.date.issued2016
dc.description.abstractInduced pluripotent stem cells (iPSCs) offer great promise as tools for basic biomedical research, disease modeling, and drug screening. In this chapter, we describe the generation of patient-specific, transgene-free iPSCs from skin biopsies and peripheral blood mononuclear cells through electroporation of episomal vectors and growth under two different culture conditions. The resulting iPSC lines are characterized with respect to pluripotency marker expression through immunostaining, tested for transgene integration by PCR, and assayed for differentiation capacity via teratoma formation.
dc.description.fulltextNo
dc.description.harvestedfromManual
dc.description.indexedbyWOS
dc.description.indexedbyPubMed
dc.description.indexedbyScopus
dc.description.openaccessNO
dc.description.peerreviewstatusN/A
dc.description.publisherscopeInternational
dc.description.readpublishN/A
dc.description.sponsoredbyTubitakEuEU - TÜBİTAK
dc.description.sponsorshipWork in our laboratory is supported by a TUBITAK 3501 grant (212T095), EMBO installation grant 2543, and FP7 Marie Curie CIG 333918 CMR.
dc.description.studentonlypublicationNo
dc.description.studentpublicationYes
dc.description.versionN/A
dc.identifier.WoSQuartileN/A
dc.identifier.doi10.1007/7651_2015_278
dc.identifier.eissn1940-6029
dc.identifier.embargoN/A
dc.identifier.endpage231
dc.identifier.grantno212T095
dc.identifier.grantno333918
dc.identifier.grantno2543
dc.identifier.isbn9781493930340
dc.identifier.isbn9781493930333
dc.identifier.isbn9781493949731
dc.identifier.issn1064-3745
dc.identifier.pubmed26126451
dc.identifier.scopus2-s2.0-84946811351
dc.identifier.startpage215
dc.identifier.urihttps://doi.org/10.1007/7651_2015_278
dc.identifier.urihttps://hdl.handle.net/20.500.14288/7287
dc.identifier.volume1353
dc.identifier.wos000696318000016
dc.keywordsInduced pluripotent stem cells
dc.keywordsiPSC
dc.keywordsDisease modeling
dc.keywordsTransgene-free
dc.keywordsEpisomal vector
dc.keywordsCharacterization of pluripotency
dc.language.isoeng
dc.publisherHumana Press
dc.relation.affiliationKoç University
dc.relation.collectionKoç University Institutional Repository
dc.relation.ispartofPatient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization
dc.relation.openaccessN/A
dc.rightsN/A
dc.subjectInduced pluripotent stem cells
dc.subjectCellular reprogramming
dc.subjectStem cell biology
dc.subjectDisease modeling
dc.titleTransgene-free disease-specific iPSC generation from fibroblasts and peripheral blood mononuclear cells
dc.typeBook Chapter
dspace.entity.typePublication
local.contributor.kuauthorFidan, Kerem
local.contributor.kuauthorBozlak, Özlem Hilal Çağlayan
local.contributor.kuauthorÖzçimen, Burcu
local.contributor.kuauthorÖnder, Tamer Tevfik
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