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Transgene-free disease-specific iPSC generation from fibroblasts and peripheral blood mononuclear cells

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dc.contributor.coauthorEbrahimi, Ayyub
dc.contributor.departmentSchool of Medicine
dc.contributor.departmentGraduate School of Sciences and Engineering
dc.contributor.kuauthorBozlak, Özlem Hilal Çağlayan
dc.contributor.kuauthorFidan, Kerem
dc.contributor.kuauthorÖnder, Tamer Tevfik
dc.contributor.kuauthorÖzçimen, Burcu
dc.contributor.schoolcollegeinstituteGRADUATE SCHOOL OF SCIENCES AND ENGINEERING
dc.contributor.schoolcollegeinstituteSCHOOL OF MEDICINE
dc.date.accessioned2024-11-09T22:55:59Z
dc.date.issued2016
dc.description.abstractInduced pluripotent stem cells (iPSCs) offer great promise as tools for basic biomedical research, disease modeling, and drug screening. In this chapter, we describe the generation of patient-specific, transgene-free iPSCs from skin biopsies and peripheral blood mononuclear cells through electroporation of episomal vectors and growth under two different culture conditions. The resulting iPSC lines are characterized with respect to pluripotency marker expression through immunostaining, tested for transgene integration by PCR, and assayed for differentiation capacity via teratoma formation.
dc.description.indexedbyWOS
dc.description.indexedbyPubMed
dc.description.openaccessNO
dc.description.publisherscopeInternational
dc.description.sponsoredbyTubitakEuN/A
dc.description.sponsorshipTUBITAK3501 grant [212T095]
dc.description.sponsorshipEMBO installation grant [2543]
dc.description.sponsorshipFP7 Marie Curie [CIG 333918 CMR] Work in our laboratory is supported by a TUBITAK3501 grant (212T095), EMBO installation grant 2543, and FP7 Marie Curie CIG 333918 CMR.
dc.description.volume1353
dc.identifier.doi10.1007/7651_2015_278
dc.identifier.eissn1940-6029
dc.identifier.isbn978-1-4939-3034-0
dc.identifier.isbn978-1-4939-3033-3
dc.identifier.issn1064-3745
dc.identifier.quartileN/A
dc.identifier.urihttps://doi.org/10.1007/7651_2015_278
dc.identifier.urihttps://hdl.handle.net/20.500.14288/7287
dc.identifier.wos696318000016
dc.keywordsInduced pluripotent stem cells
dc.keywordsiPSC
dc.keywordsDisease modeling
dc.keywordsTransgene-free
dc.keywordsEpisomal vector
dc.keywordsCharacterization of pluripotency
dc.language.isoeng
dc.publisherHumana Press Inc
dc.relation.ispartofPatient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization
dc.subjectBiochemical research methods
dc.subjectCell biology
dc.subjectMedicine
dc.subjectResearch and experimental
dc.titleTransgene-free disease-specific iPSC generation from fibroblasts and peripheral blood mononuclear cells
dc.typeBook Chapter
dspace.entity.typePublication
local.contributor.kuauthorFidan, Kerem
local.contributor.kuauthorBozlak, Özlem Hilal Çağlayan
local.contributor.kuauthorÖzçimen, Burcu
local.contributor.kuauthorÖnder, Tamer Tevfik
local.publication.orgunit1GRADUATE SCHOOL OF SCIENCES AND ENGINEERING
local.publication.orgunit1SCHOOL OF MEDICINE
local.publication.orgunit2School of Medicine
local.publication.orgunit2Graduate School of Sciences and Engineering
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