Publication:
Transgene-free disease-specific iPSC generation from fibroblasts and peripheral blood mononuclear cells

Placeholder

School / College / Institute

Organizational Unit
Organizational Unit
SCHOOL OF MEDICINE
Upper Org Unit

Program

KU Authors

Co-Authors

Ebrahimi, Ayyub

Publication Date

Language

Embargo Status

Journal Title

Journal ISSN

Volume Title

Alternative Title

Abstract

Induced pluripotent stem cells (iPSCs) offer great promise as tools for basic biomedical research, disease modeling, and drug screening. In this chapter, we describe the generation of patient-specific, transgene-free iPSCs from skin biopsies and peripheral blood mononuclear cells through electroporation of episomal vectors and growth under two different culture conditions. The resulting iPSC lines are characterized with respect to pluripotency marker expression through immunostaining, tested for transgene integration by PCR, and assayed for differentiation capacity via teratoma formation.

Source

Publisher

Humana Press Inc

Subject

Biochemical research methods, Cell biology, Medicine, Research and experimental

Citation

Has Part

Source

Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization

Book Series Title

Edition

DOI

10.1007/7651_2015_278

item.page.datauri

Link

Rights

Copyrights Note

Endorsement

Review

Supplemented By

Referenced By

0

Views

0

Downloads

View PlumX Details