Publication: Transgene-free disease-specific iPSC generation from fibroblasts and peripheral blood mononuclear cells
Program
KU Authors
Co-Authors
Ebrahimi, Ayyub
Publication Date
Language
Type
Embargo Status
Journal Title
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Volume Title
Alternative Title
Abstract
Induced pluripotent stem cells (iPSCs) offer great promise as tools for basic biomedical research, disease modeling, and drug screening. In this chapter, we describe the generation of patient-specific, transgene-free iPSCs from skin biopsies and peripheral blood mononuclear cells through electroporation of episomal vectors and growth under two different culture conditions. The resulting iPSC lines are characterized with respect to pluripotency marker expression through immunostaining, tested for transgene integration by PCR, and assayed for differentiation capacity via teratoma formation.
Source
Publisher
Humana Press Inc
Subject
Biochemical research methods, Cell biology, Medicine, Research and experimental
Citation
Has Part
Source
Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization
Book Series Title
Edition
DOI
10.1007/7651_2015_278